Identification of larval Contracaecum osculatum s.l. and Phocascaris sp. (Nematoda: Ascaridoidea) from marine fishes by allozyme electrophoresis and discriminant function analysis of morphometric data
Biochemical markers (allozymes) were used to identify third-stage larvae of nematodes from the Contracaecum osculatum s.l. – Phocascaris sp. complex found in five marine fish species (Hippoglossoides platessoides, Gadus morhua, Mallotus villosus, Myoxocephalus scorpius, Pleuronectes americanus) coll...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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Canadian Science Publishing
1995
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Online Access: | http://dx.doi.org/10.1139/f95-517 http://www.nrcresearchpress.com/doi/pdf/10.1139/f95-517 |
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crcansciencepubl:10.1139/f95-517 2023-12-17T10:30:24+01:00 Identification of larval Contracaecum osculatum s.l. and Phocascaris sp. (Nematoda: Ascaridoidea) from marine fishes by allozyme electrophoresis and discriminant function analysis of morphometric data Brattey, John 1995 http://dx.doi.org/10.1139/f95-517 http://www.nrcresearchpress.com/doi/pdf/10.1139/f95-517 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 52, issue S1, page 116-128 ISSN 0706-652X 1205-7533 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 1995 crcansciencepubl https://doi.org/10.1139/f95-517 2023-11-19T13:38:26Z Biochemical markers (allozymes) were used to identify third-stage larvae of nematodes from the Contracaecum osculatum s.l. – Phocascaris sp. complex found in five marine fish species (Hippoglossoides platessoides, Gadus morhua, Mallotus villosus, Myoxocephalus scorpius, Pleuronectes americanus) collected off Newfoundland and Labrador. Three taxonomic groups, genetically identical to adult C. osculatum A, C. osculatum B, and Phocascaris sp. previously reported from North Atlantic seals, were found. Among the 859 larvae examined, C. osculatum B was most common (74.2%), particularly in H. platessoides (79.4% of 501 larvae), G. morhua (88.8% of 160), M. scorpius (87.5% of 32), and P. americanus (100.0% of 20), but Phocascaris sp. was more numerous in M. villosus (66.4% of 146). Contracaecum osculatum A was found only in H. platessoides (15% of 501). Discriminant function analysis revealed differences in shape among the nematode species. Larvae of Phocascaris sp. and C. osculatum B can generally be distinguished using three measurements and classified according to coefficients for quadratic discriminant functions, but larvae of C. osculatum A could not be reliably distinguished from the other two species. A simpler, more rapid method of screening biochemical markers, employing a cellulose acetate support medium, is also described. Article in Journal/Newspaper Gadus morhua Newfoundland North Atlantic Canadian Science Publishing (via Crossref) Newfoundland Canadian Journal of Fisheries and Aquatic Sciences 52 S1 116 128 |
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Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics Brattey, John Identification of larval Contracaecum osculatum s.l. and Phocascaris sp. (Nematoda: Ascaridoidea) from marine fishes by allozyme electrophoresis and discriminant function analysis of morphometric data |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
Biochemical markers (allozymes) were used to identify third-stage larvae of nematodes from the Contracaecum osculatum s.l. – Phocascaris sp. complex found in five marine fish species (Hippoglossoides platessoides, Gadus morhua, Mallotus villosus, Myoxocephalus scorpius, Pleuronectes americanus) collected off Newfoundland and Labrador. Three taxonomic groups, genetically identical to adult C. osculatum A, C. osculatum B, and Phocascaris sp. previously reported from North Atlantic seals, were found. Among the 859 larvae examined, C. osculatum B was most common (74.2%), particularly in H. platessoides (79.4% of 501 larvae), G. morhua (88.8% of 160), M. scorpius (87.5% of 32), and P. americanus (100.0% of 20), but Phocascaris sp. was more numerous in M. villosus (66.4% of 146). Contracaecum osculatum A was found only in H. platessoides (15% of 501). Discriminant function analysis revealed differences in shape among the nematode species. Larvae of Phocascaris sp. and C. osculatum B can generally be distinguished using three measurements and classified according to coefficients for quadratic discriminant functions, but larvae of C. osculatum A could not be reliably distinguished from the other two species. A simpler, more rapid method of screening biochemical markers, employing a cellulose acetate support medium, is also described. |
format |
Article in Journal/Newspaper |
author |
Brattey, John |
author_facet |
Brattey, John |
author_sort |
Brattey, John |
title |
Identification of larval Contracaecum osculatum s.l. and Phocascaris sp. (Nematoda: Ascaridoidea) from marine fishes by allozyme electrophoresis and discriminant function analysis of morphometric data |
title_short |
Identification of larval Contracaecum osculatum s.l. and Phocascaris sp. (Nematoda: Ascaridoidea) from marine fishes by allozyme electrophoresis and discriminant function analysis of morphometric data |
title_full |
Identification of larval Contracaecum osculatum s.l. and Phocascaris sp. (Nematoda: Ascaridoidea) from marine fishes by allozyme electrophoresis and discriminant function analysis of morphometric data |
title_fullStr |
Identification of larval Contracaecum osculatum s.l. and Phocascaris sp. (Nematoda: Ascaridoidea) from marine fishes by allozyme electrophoresis and discriminant function analysis of morphometric data |
title_full_unstemmed |
Identification of larval Contracaecum osculatum s.l. and Phocascaris sp. (Nematoda: Ascaridoidea) from marine fishes by allozyme electrophoresis and discriminant function analysis of morphometric data |
title_sort |
identification of larval contracaecum osculatum s.l. and phocascaris sp. (nematoda: ascaridoidea) from marine fishes by allozyme electrophoresis and discriminant function analysis of morphometric data |
publisher |
Canadian Science Publishing |
publishDate |
1995 |
url |
http://dx.doi.org/10.1139/f95-517 http://www.nrcresearchpress.com/doi/pdf/10.1139/f95-517 |
geographic |
Newfoundland |
geographic_facet |
Newfoundland |
genre |
Gadus morhua Newfoundland North Atlantic |
genre_facet |
Gadus morhua Newfoundland North Atlantic |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 52, issue S1, page 116-128 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f95-517 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
container_volume |
52 |
container_issue |
S1 |
container_start_page |
116 |
op_container_end_page |
128 |
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1785583361922695168 |