Phylogenetic Relationships among Members of the Coregoninae Inferred from Direct Sequencing of PCR-Amplified Mitochondrial DNA
We determined the DNA sequence of a portion of the mitochondrial cytochrome b gene for eight members of the Coregoninae (Salmonidae) from North America in an effort to elucidate phylogenetic relationships in the subfamily. DNA was prepared for sequencing by the polymerase chain reaction (PCR). Direc...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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Online Access: | http://dx.doi.org/10.1139/f93-236 http://www.nrcresearchpress.com/doi/pdf/10.1139/f93-236 |
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crcansciencepubl:10.1139/f93-236 2024-05-19T07:33:47+00:00 Phylogenetic Relationships among Members of the Coregoninae Inferred from Direct Sequencing of PCR-Amplified Mitochondrial DNA Lockwood, S. Fournier Dillinger Jr., Robert E. Birt, Tim P. Green, John M. Snyder, Thomas P. 1993 http://dx.doi.org/10.1139/f93-236 http://www.nrcresearchpress.com/doi/pdf/10.1139/f93-236 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 50, issue 10, page 2112-2118 ISSN 0706-652X 1205-7533 journal-article 1993 crcansciencepubl https://doi.org/10.1139/f93-236 2024-05-02T06:51:25Z We determined the DNA sequence of a portion of the mitochondrial cytochrome b gene for eight members of the Coregoninae (Salmonidae) from North America in an effort to elucidate phylogenetic relationships in the subfamily. DNA was prepared for sequencing by the polymerase chain reaction (PCR). Direct estimates of mitochondrial DNA (mtDNA) sequence divergence among taxa ranged from 0.0% between Arctic cisco (Coregonus autumnalis) from the Mackenzie River, Canada, and cisco (C. artedi) from the Laurentian Great Lakes to 5.8% between the inconnu (Stenodus leucichthys) and the round whitefish (Prosopium cylindraceum). As has been noted in other fish species and vertebrates in general, third position silent substitutions predominated over any other type of nucleotide change. No amino acid replacement substitutions were found among any of the eight taxa examined. Comparison of mtDNA sequence divergence estimates from this and other studies suggests that the radiation of the genera within the Coregoninae occurred relatively recently (2–6 million yr B.P.). Cladistic analysis of the mtDNA sequence data yields a hypothesis of relationships that supports previous genetic and morphological classifications of coregonines. Article in Journal/Newspaper Arctic cisco Arctic Coregonus autumnalis Mackenzie river Canadian Science Publishing Canadian Journal of Fisheries and Aquatic Sciences 50 10 2112 2118 |
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Open Polar |
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Canadian Science Publishing |
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crcansciencepubl |
language |
English |
description |
We determined the DNA sequence of a portion of the mitochondrial cytochrome b gene for eight members of the Coregoninae (Salmonidae) from North America in an effort to elucidate phylogenetic relationships in the subfamily. DNA was prepared for sequencing by the polymerase chain reaction (PCR). Direct estimates of mitochondrial DNA (mtDNA) sequence divergence among taxa ranged from 0.0% between Arctic cisco (Coregonus autumnalis) from the Mackenzie River, Canada, and cisco (C. artedi) from the Laurentian Great Lakes to 5.8% between the inconnu (Stenodus leucichthys) and the round whitefish (Prosopium cylindraceum). As has been noted in other fish species and vertebrates in general, third position silent substitutions predominated over any other type of nucleotide change. No amino acid replacement substitutions were found among any of the eight taxa examined. Comparison of mtDNA sequence divergence estimates from this and other studies suggests that the radiation of the genera within the Coregoninae occurred relatively recently (2–6 million yr B.P.). Cladistic analysis of the mtDNA sequence data yields a hypothesis of relationships that supports previous genetic and morphological classifications of coregonines. |
format |
Article in Journal/Newspaper |
author |
Lockwood, S. Fournier Dillinger Jr., Robert E. Birt, Tim P. Green, John M. Snyder, Thomas P. |
spellingShingle |
Lockwood, S. Fournier Dillinger Jr., Robert E. Birt, Tim P. Green, John M. Snyder, Thomas P. Phylogenetic Relationships among Members of the Coregoninae Inferred from Direct Sequencing of PCR-Amplified Mitochondrial DNA |
author_facet |
Lockwood, S. Fournier Dillinger Jr., Robert E. Birt, Tim P. Green, John M. Snyder, Thomas P. |
author_sort |
Lockwood, S. Fournier |
title |
Phylogenetic Relationships among Members of the Coregoninae Inferred from Direct Sequencing of PCR-Amplified Mitochondrial DNA |
title_short |
Phylogenetic Relationships among Members of the Coregoninae Inferred from Direct Sequencing of PCR-Amplified Mitochondrial DNA |
title_full |
Phylogenetic Relationships among Members of the Coregoninae Inferred from Direct Sequencing of PCR-Amplified Mitochondrial DNA |
title_fullStr |
Phylogenetic Relationships among Members of the Coregoninae Inferred from Direct Sequencing of PCR-Amplified Mitochondrial DNA |
title_full_unstemmed |
Phylogenetic Relationships among Members of the Coregoninae Inferred from Direct Sequencing of PCR-Amplified Mitochondrial DNA |
title_sort |
phylogenetic relationships among members of the coregoninae inferred from direct sequencing of pcr-amplified mitochondrial dna |
publisher |
Canadian Science Publishing |
publishDate |
1993 |
url |
http://dx.doi.org/10.1139/f93-236 http://www.nrcresearchpress.com/doi/pdf/10.1139/f93-236 |
genre |
Arctic cisco Arctic Coregonus autumnalis Mackenzie river |
genre_facet |
Arctic cisco Arctic Coregonus autumnalis Mackenzie river |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 50, issue 10, page 2112-2118 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f93-236 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
container_volume |
50 |
container_issue |
10 |
container_start_page |
2112 |
op_container_end_page |
2118 |
_version_ |
1799471858161025024 |