Use of Biochemical Genetic Markers to Discriminate between Adductor Muscles of the Sea Scallop ( Placopecten magellanicus) and the Iceland Scallop ( Chlamys islandica)
Three biochemical techniques were applied to adductor muscles of Placopecten magellanicus and Chlamys islandica, two commercially important scallops, to search for species-specific genetic markers. Allozyme electrophoresis identified two enzyme systems, mannose phosphate isomerase and glutamic oxalo...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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Language: | English |
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Canadian Science Publishing
1993
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Online Access: | http://dx.doi.org/10.1139/f93-138 http://www.nrcresearchpress.com/doi/pdf/10.1139/f93-138 |
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crcansciencepubl:10.1139/f93-138 2024-09-09T19:46:49+00:00 Use of Biochemical Genetic Markers to Discriminate between Adductor Muscles of the Sea Scallop ( Placopecten magellanicus) and the Iceland Scallop ( Chlamys islandica) Kenchington, E. Naidu, K. S. Roddick, D. L. Cook, D. I. Zouros, E. 1993 http://dx.doi.org/10.1139/f93-138 http://www.nrcresearchpress.com/doi/pdf/10.1139/f93-138 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 50, issue 6, page 1222-1228 ISSN 0706-652X 1205-7533 journal-article 1993 crcansciencepubl https://doi.org/10.1139/f93-138 2024-07-25T04:10:05Z Three biochemical techniques were applied to adductor muscles of Placopecten magellanicus and Chlamys islandica, two commercially important scallops, to search for species-specific genetic markers. Allozyme electrophoresis identified two enzyme systems, mannose phosphate isomerase and glutamic oxaloacetate transaminase, which appear to be diagnostic. Sequencing of the 18S rRNA gene in each species showed 15 nucleotide differences over 1815 base pairs. Digestion of the gene with the restriction enzyme XHO I released two fragments in Placopecten and three in Chlamys. All three techniques could be developed for management purposes as part of an enforcement programme to identify illegally caught scallops. Article in Journal/Newspaper Iceland Canadian Science Publishing Canadian Journal of Fisheries and Aquatic Sciences 50 6 1222 1228 |
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Open Polar |
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Canadian Science Publishing |
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crcansciencepubl |
language |
English |
description |
Three biochemical techniques were applied to adductor muscles of Placopecten magellanicus and Chlamys islandica, two commercially important scallops, to search for species-specific genetic markers. Allozyme electrophoresis identified two enzyme systems, mannose phosphate isomerase and glutamic oxaloacetate transaminase, which appear to be diagnostic. Sequencing of the 18S rRNA gene in each species showed 15 nucleotide differences over 1815 base pairs. Digestion of the gene with the restriction enzyme XHO I released two fragments in Placopecten and three in Chlamys. All three techniques could be developed for management purposes as part of an enforcement programme to identify illegally caught scallops. |
format |
Article in Journal/Newspaper |
author |
Kenchington, E. Naidu, K. S. Roddick, D. L. Cook, D. I. Zouros, E. |
spellingShingle |
Kenchington, E. Naidu, K. S. Roddick, D. L. Cook, D. I. Zouros, E. Use of Biochemical Genetic Markers to Discriminate between Adductor Muscles of the Sea Scallop ( Placopecten magellanicus) and the Iceland Scallop ( Chlamys islandica) |
author_facet |
Kenchington, E. Naidu, K. S. Roddick, D. L. Cook, D. I. Zouros, E. |
author_sort |
Kenchington, E. |
title |
Use of Biochemical Genetic Markers to Discriminate between Adductor Muscles of the Sea Scallop ( Placopecten magellanicus) and the Iceland Scallop ( Chlamys islandica) |
title_short |
Use of Biochemical Genetic Markers to Discriminate between Adductor Muscles of the Sea Scallop ( Placopecten magellanicus) and the Iceland Scallop ( Chlamys islandica) |
title_full |
Use of Biochemical Genetic Markers to Discriminate between Adductor Muscles of the Sea Scallop ( Placopecten magellanicus) and the Iceland Scallop ( Chlamys islandica) |
title_fullStr |
Use of Biochemical Genetic Markers to Discriminate between Adductor Muscles of the Sea Scallop ( Placopecten magellanicus) and the Iceland Scallop ( Chlamys islandica) |
title_full_unstemmed |
Use of Biochemical Genetic Markers to Discriminate between Adductor Muscles of the Sea Scallop ( Placopecten magellanicus) and the Iceland Scallop ( Chlamys islandica) |
title_sort |
use of biochemical genetic markers to discriminate between adductor muscles of the sea scallop ( placopecten magellanicus) and the iceland scallop ( chlamys islandica) |
publisher |
Canadian Science Publishing |
publishDate |
1993 |
url |
http://dx.doi.org/10.1139/f93-138 http://www.nrcresearchpress.com/doi/pdf/10.1139/f93-138 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 50, issue 6, page 1222-1228 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f93-138 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
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50 |
container_issue |
6 |
container_start_page |
1222 |
op_container_end_page |
1228 |
_version_ |
1809916318097342464 |