Use of Biochemical Genetic Markers to Discriminate between Adductor Muscles of the Sea Scallop ( Placopecten magellanicus ) and the Iceland Scallop ( Chlamys islandica )
Three biochemical techniques were applied to adductor muscles of Placopecten magellanicus and Chlamys islandica, two commercially important scallops, to search for species-specific genetic markers. Allozyme electrophoresis identified two enzyme systems, mannose phosphate isomerase and glutamic oxalo...
| Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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| Main Authors: | , , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Canadian Science Publishing
1993
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1139/f93-138 http://www.nrcresearchpress.com/doi/pdf/10.1139/f93-138 |
| Summary: | Three biochemical techniques were applied to adductor muscles of Placopecten magellanicus and Chlamys islandica, two commercially important scallops, to search for species-specific genetic markers. Allozyme electrophoresis identified two enzyme systems, mannose phosphate isomerase and glutamic oxaloacetate transaminase, which appear to be diagnostic. Sequencing of the 18S rRNA gene in each species showed 15 nucleotide differences over 1815 base pairs. Digestion of the gene with the restriction enzyme XHO I released two fragments in Placopecten and three in Chlamys. All three techniques could be developed for management purposes as part of an enforcement programme to identify illegally caught scallops. |
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