Mitochondrial DNA Variation in Chinook ( Oncorhynchus tshawytscha ) and Chum Salmon ( O . keta ) Detected by Restriction Enzyme Analysis of Polymerase Chain Reaction (PCR) Products
We analyzed intraspecific mitochondrial DNA variation in chinook salmon (Oncorhynchus tshawytscha) from drainages in the Yukon River (Alaska and Yukon Territory), the Kenai River (Alaska), and Oregon and California rivers; and chum salmon (O. keta) from the Yukon River and Vancouver Island, and Wash...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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1993
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Online Access: | http://dx.doi.org/10.1139/f93-081 http://www.nrcresearchpress.com/doi/pdf/10.1139/f93-081 |
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crcansciencepubl:10.1139/f93-081 2023-12-17T10:51:32+01:00 Mitochondrial DNA Variation in Chinook ( Oncorhynchus tshawytscha ) and Chum Salmon ( O . keta ) Detected by Restriction Enzyme Analysis of Polymerase Chain Reaction (PCR) Products Cronin, Matthew A. Spearman, William J. Wilmot, Richard L. Patton, John C. Bickham, John W. 1993 http://dx.doi.org/10.1139/f93-081 http://www.nrcresearchpress.com/doi/pdf/10.1139/f93-081 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 50, issue 4, page 708-715 ISSN 0706-652X 1205-7533 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 1993 crcansciencepubl https://doi.org/10.1139/f93-081 2023-11-19T13:38:24Z We analyzed intraspecific mitochondrial DNA variation in chinook salmon (Oncorhynchus tshawytscha) from drainages in the Yukon River (Alaska and Yukon Territory), the Kenai River (Alaska), and Oregon and California rivers; and chum salmon (O. keta) from the Yukon River and Vancouver Island, and Washington rivers. For each species, three different portions of the mtDNA molecule were amplified separately using the polymerase chain reaction and then digested with at least 19 restriction enzymes. Intraspecific sequence divergences between haplotypes were less than 0.01 base substitution per nucleotide. Nine chum salmon haplotypes were identified. Yukon River chum salmon stocks displayed more haplotypes (eight) than the stocks of Vancouver Island and Washington (two). The most common chum salmon haplotype occurred in all areas. Seven chinook salmon haplotypes were identified. Four haplotypes occurred in the Yukon and Kenai rivers and four occurred in Oregon/California, with only one haplotype shared between the regions. Sample sizes were too small to quantify the degree of stock separation among drainages, but the patterns of variation that we observed suggest utility of the technique in genetic stock identification. Article in Journal/Newspaper Yukon river Alaska Yukon Canadian Science Publishing (via Crossref) Yukon Keta ENVELOPE(-19.455,-19.455,65.656,65.656) Canadian Journal of Fisheries and Aquatic Sciences 50 4 708 715 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics Cronin, Matthew A. Spearman, William J. Wilmot, Richard L. Patton, John C. Bickham, John W. Mitochondrial DNA Variation in Chinook ( Oncorhynchus tshawytscha ) and Chum Salmon ( O . keta ) Detected by Restriction Enzyme Analysis of Polymerase Chain Reaction (PCR) Products |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
We analyzed intraspecific mitochondrial DNA variation in chinook salmon (Oncorhynchus tshawytscha) from drainages in the Yukon River (Alaska and Yukon Territory), the Kenai River (Alaska), and Oregon and California rivers; and chum salmon (O. keta) from the Yukon River and Vancouver Island, and Washington rivers. For each species, three different portions of the mtDNA molecule were amplified separately using the polymerase chain reaction and then digested with at least 19 restriction enzymes. Intraspecific sequence divergences between haplotypes were less than 0.01 base substitution per nucleotide. Nine chum salmon haplotypes were identified. Yukon River chum salmon stocks displayed more haplotypes (eight) than the stocks of Vancouver Island and Washington (two). The most common chum salmon haplotype occurred in all areas. Seven chinook salmon haplotypes were identified. Four haplotypes occurred in the Yukon and Kenai rivers and four occurred in Oregon/California, with only one haplotype shared between the regions. Sample sizes were too small to quantify the degree of stock separation among drainages, but the patterns of variation that we observed suggest utility of the technique in genetic stock identification. |
format |
Article in Journal/Newspaper |
author |
Cronin, Matthew A. Spearman, William J. Wilmot, Richard L. Patton, John C. Bickham, John W. |
author_facet |
Cronin, Matthew A. Spearman, William J. Wilmot, Richard L. Patton, John C. Bickham, John W. |
author_sort |
Cronin, Matthew A. |
title |
Mitochondrial DNA Variation in Chinook ( Oncorhynchus tshawytscha ) and Chum Salmon ( O . keta ) Detected by Restriction Enzyme Analysis of Polymerase Chain Reaction (PCR) Products |
title_short |
Mitochondrial DNA Variation in Chinook ( Oncorhynchus tshawytscha ) and Chum Salmon ( O . keta ) Detected by Restriction Enzyme Analysis of Polymerase Chain Reaction (PCR) Products |
title_full |
Mitochondrial DNA Variation in Chinook ( Oncorhynchus tshawytscha ) and Chum Salmon ( O . keta ) Detected by Restriction Enzyme Analysis of Polymerase Chain Reaction (PCR) Products |
title_fullStr |
Mitochondrial DNA Variation in Chinook ( Oncorhynchus tshawytscha ) and Chum Salmon ( O . keta ) Detected by Restriction Enzyme Analysis of Polymerase Chain Reaction (PCR) Products |
title_full_unstemmed |
Mitochondrial DNA Variation in Chinook ( Oncorhynchus tshawytscha ) and Chum Salmon ( O . keta ) Detected by Restriction Enzyme Analysis of Polymerase Chain Reaction (PCR) Products |
title_sort |
mitochondrial dna variation in chinook ( oncorhynchus tshawytscha ) and chum salmon ( o . keta ) detected by restriction enzyme analysis of polymerase chain reaction (pcr) products |
publisher |
Canadian Science Publishing |
publishDate |
1993 |
url |
http://dx.doi.org/10.1139/f93-081 http://www.nrcresearchpress.com/doi/pdf/10.1139/f93-081 |
long_lat |
ENVELOPE(-19.455,-19.455,65.656,65.656) |
geographic |
Yukon Keta |
geographic_facet |
Yukon Keta |
genre |
Yukon river Alaska Yukon |
genre_facet |
Yukon river Alaska Yukon |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 50, issue 4, page 708-715 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f93-081 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
container_volume |
50 |
container_issue |
4 |
container_start_page |
708 |
op_container_end_page |
715 |
_version_ |
1785576818301992960 |