A Polymorphism in the Ribosomal RNA Genes Distinguishes Atlantic Salmon ( Salmo salar ) from North America and Europe
There is good evidence to suggest that Atlantic salmon (Salmo salar) from North America and Europe form distinct breeding populations. However, it has been very difficult to discover a genetic marker that allows the continent of origin of an individual salmon to be determined unambiguously. Repetiti...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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1991
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Online Access: | http://dx.doi.org/10.1139/f91-196 http://www.nrcresearchpress.com/doi/pdf/10.1139/f91-196 |
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crcansciencepubl:10.1139/f91-196 2023-12-17T10:27:08+01:00 A Polymorphism in the Ribosomal RNA Genes Distinguishes Atlantic Salmon ( Salmo salar ) from North America and Europe Cutler, Michael G. Bartlett, Sylvia E. Hartley, Sheila E. Davidson, William S. 1991 http://dx.doi.org/10.1139/f91-196 http://www.nrcresearchpress.com/doi/pdf/10.1139/f91-196 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 48, issue 9, page 1655-1661 ISSN 0706-652X 1205-7533 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 1991 crcansciencepubl https://doi.org/10.1139/f91-196 2023-11-19T13:38:29Z There is good evidence to suggest that Atlantic salmon (Salmo salar) from North America and Europe form distinct breeding populations. However, it has been very difficult to discover a genetic marker that allows the continent of origin of an individual salmon to be determined unambiguously. Repetitive elements in the nuclear genome have provided population-specific markers in several species. Therefore, we examined the ribosomal RNA gene (rDNA) complex in Atlantic salmon by Southern blot analysis using a cloned repeating unit of the Drosophila melanogaster rDNA as a probe. A restriction fragment length polymorphism that could distinguish between salmon from Newfoundland and Scotland was detected using a combination of the restriction enzymes SacI and XbaI. The Newfoundland salmon had additional fragments of 3.6 kilobase pairs (kbp) and 2.6 kbp. Of 27 salmon from 10 populations in Europe, none possessed the 3.6- or the 2.6-kbp fragment whereas all 33 North American Atlantic salmon (from six populations) had the 2.6-kbp band and the majority showed the 3.6-kbp fragment. This preliminary survey strongly suggests that this genetic marker can be used to identify the continent of origin of individual Atlantic salmon. Article in Journal/Newspaper Atlantic salmon Newfoundland Salmo salar Canadian Science Publishing (via Crossref) Canadian Journal of Fisheries and Aquatic Sciences 48 9 1655 1661 |
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Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics Cutler, Michael G. Bartlett, Sylvia E. Hartley, Sheila E. Davidson, William S. A Polymorphism in the Ribosomal RNA Genes Distinguishes Atlantic Salmon ( Salmo salar ) from North America and Europe |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
There is good evidence to suggest that Atlantic salmon (Salmo salar) from North America and Europe form distinct breeding populations. However, it has been very difficult to discover a genetic marker that allows the continent of origin of an individual salmon to be determined unambiguously. Repetitive elements in the nuclear genome have provided population-specific markers in several species. Therefore, we examined the ribosomal RNA gene (rDNA) complex in Atlantic salmon by Southern blot analysis using a cloned repeating unit of the Drosophila melanogaster rDNA as a probe. A restriction fragment length polymorphism that could distinguish between salmon from Newfoundland and Scotland was detected using a combination of the restriction enzymes SacI and XbaI. The Newfoundland salmon had additional fragments of 3.6 kilobase pairs (kbp) and 2.6 kbp. Of 27 salmon from 10 populations in Europe, none possessed the 3.6- or the 2.6-kbp fragment whereas all 33 North American Atlantic salmon (from six populations) had the 2.6-kbp band and the majority showed the 3.6-kbp fragment. This preliminary survey strongly suggests that this genetic marker can be used to identify the continent of origin of individual Atlantic salmon. |
format |
Article in Journal/Newspaper |
author |
Cutler, Michael G. Bartlett, Sylvia E. Hartley, Sheila E. Davidson, William S. |
author_facet |
Cutler, Michael G. Bartlett, Sylvia E. Hartley, Sheila E. Davidson, William S. |
author_sort |
Cutler, Michael G. |
title |
A Polymorphism in the Ribosomal RNA Genes Distinguishes Atlantic Salmon ( Salmo salar ) from North America and Europe |
title_short |
A Polymorphism in the Ribosomal RNA Genes Distinguishes Atlantic Salmon ( Salmo salar ) from North America and Europe |
title_full |
A Polymorphism in the Ribosomal RNA Genes Distinguishes Atlantic Salmon ( Salmo salar ) from North America and Europe |
title_fullStr |
A Polymorphism in the Ribosomal RNA Genes Distinguishes Atlantic Salmon ( Salmo salar ) from North America and Europe |
title_full_unstemmed |
A Polymorphism in the Ribosomal RNA Genes Distinguishes Atlantic Salmon ( Salmo salar ) from North America and Europe |
title_sort |
polymorphism in the ribosomal rna genes distinguishes atlantic salmon ( salmo salar ) from north america and europe |
publisher |
Canadian Science Publishing |
publishDate |
1991 |
url |
http://dx.doi.org/10.1139/f91-196 http://www.nrcresearchpress.com/doi/pdf/10.1139/f91-196 |
genre |
Atlantic salmon Newfoundland Salmo salar |
genre_facet |
Atlantic salmon Newfoundland Salmo salar |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 48, issue 9, page 1655-1661 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f91-196 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
container_volume |
48 |
container_issue |
9 |
container_start_page |
1655 |
op_container_end_page |
1661 |
_version_ |
1785578916852793344 |