Evidence for Antifreeze Protein Gene Transfer in Atlantic Salmon ( Salmo salar)
Atlantic salmon (Salmo salar) freeze to death if they come into contact with ice at water temperatures below −0.7 °C. Consequently, sea-pen culture of this species in cold water is severely limited. Winter flounder (Pseudopleuronectes americanus) survive in ice-laden seawater by producing a set of a...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Canadian Science Publishing
1988
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1139/f88-042 http://www.nrcresearchpress.com/doi/pdf/10.1139/f88-042 |
id |
crcansciencepubl:10.1139/f88-042 |
---|---|
record_format |
openpolar |
spelling |
crcansciencepubl:10.1139/f88-042 2024-09-15T17:55:56+00:00 Evidence for Antifreeze Protein Gene Transfer in Atlantic Salmon ( Salmo salar) Fletcher, Garth L. Shears, Margaret A. King, Madonna J. Davies, Peter L. Hew, Choy L. 1988 http://dx.doi.org/10.1139/f88-042 http://www.nrcresearchpress.com/doi/pdf/10.1139/f88-042 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 45, issue 2, page 352-357 ISSN 0706-652X 1205-7533 journal-article 1988 crcansciencepubl https://doi.org/10.1139/f88-042 2024-07-25T04:10:03Z Atlantic salmon (Salmo salar) freeze to death if they come into contact with ice at water temperatures below −0.7 °C. Consequently, sea-pen culture of this species in cold water is severely limited. Winter flounder (Pseudopleuronectes americanus) survive in ice-laden seawater by producing a set of antifreeze polypeptides (AFP). We are attempting to make the Atlantic salmon more freeze resistant by transferring antifreeze protein genes from the winter flounder to the genome of the salmon. Salmon eggs were microinjected with linearized DNA after fertilization. Individual fingerlings (1–2 g) were analyzed for flounder AFP genes by genomic Southern blotting. DNA from 2 out of 30 fingerlings showed hybridization to the flounder DNA probe. Hybridization bands following cleavage by restriction enzymes Sst l and Bam HI were identical to those of the injected DNA. Hybridization following Hind III digestion indicated that the flounder AFP gene was linked to the salmon genome. These hybridization signals were absent in the DNA from control fish. The intensity of the hybridization signals indicated that there was on average at least one copy of the AFP gene present per cell. Article in Journal/Newspaper Atlantic salmon Salmo salar Canadian Science Publishing Canadian Journal of Fisheries and Aquatic Sciences 45 2 352 357 |
institution |
Open Polar |
collection |
Canadian Science Publishing |
op_collection_id |
crcansciencepubl |
language |
English |
description |
Atlantic salmon (Salmo salar) freeze to death if they come into contact with ice at water temperatures below −0.7 °C. Consequently, sea-pen culture of this species in cold water is severely limited. Winter flounder (Pseudopleuronectes americanus) survive in ice-laden seawater by producing a set of antifreeze polypeptides (AFP). We are attempting to make the Atlantic salmon more freeze resistant by transferring antifreeze protein genes from the winter flounder to the genome of the salmon. Salmon eggs were microinjected with linearized DNA after fertilization. Individual fingerlings (1–2 g) were analyzed for flounder AFP genes by genomic Southern blotting. DNA from 2 out of 30 fingerlings showed hybridization to the flounder DNA probe. Hybridization bands following cleavage by restriction enzymes Sst l and Bam HI were identical to those of the injected DNA. Hybridization following Hind III digestion indicated that the flounder AFP gene was linked to the salmon genome. These hybridization signals were absent in the DNA from control fish. The intensity of the hybridization signals indicated that there was on average at least one copy of the AFP gene present per cell. |
format |
Article in Journal/Newspaper |
author |
Fletcher, Garth L. Shears, Margaret A. King, Madonna J. Davies, Peter L. Hew, Choy L. |
spellingShingle |
Fletcher, Garth L. Shears, Margaret A. King, Madonna J. Davies, Peter L. Hew, Choy L. Evidence for Antifreeze Protein Gene Transfer in Atlantic Salmon ( Salmo salar) |
author_facet |
Fletcher, Garth L. Shears, Margaret A. King, Madonna J. Davies, Peter L. Hew, Choy L. |
author_sort |
Fletcher, Garth L. |
title |
Evidence for Antifreeze Protein Gene Transfer in Atlantic Salmon ( Salmo salar) |
title_short |
Evidence for Antifreeze Protein Gene Transfer in Atlantic Salmon ( Salmo salar) |
title_full |
Evidence for Antifreeze Protein Gene Transfer in Atlantic Salmon ( Salmo salar) |
title_fullStr |
Evidence for Antifreeze Protein Gene Transfer in Atlantic Salmon ( Salmo salar) |
title_full_unstemmed |
Evidence for Antifreeze Protein Gene Transfer in Atlantic Salmon ( Salmo salar) |
title_sort |
evidence for antifreeze protein gene transfer in atlantic salmon ( salmo salar) |
publisher |
Canadian Science Publishing |
publishDate |
1988 |
url |
http://dx.doi.org/10.1139/f88-042 http://www.nrcresearchpress.com/doi/pdf/10.1139/f88-042 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 45, issue 2, page 352-357 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f88-042 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
container_volume |
45 |
container_issue |
2 |
container_start_page |
352 |
op_container_end_page |
357 |
_version_ |
1810432147936247808 |