Identification of Marine Fish Eggs: A Biochemical Genetics Approach
We studied inter- and intra-specific variation at LDH (lactate dehydrogenase; EC 1.1.1.27) loci in 11 gadoid and 6 flatfish species regularly occurring in Norwegian coastal waters by means of isoelectric focusing and histochemical staining techniques. No two species showed identical locus A (white s...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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Canadian Science Publishing
1983
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crcansciencepubl:10.1139/f83-052 2023-12-17T10:47:29+01:00 Identification of Marine Fish Eggs: A Biochemical Genetics Approach Mork, Jarle Solemdal, Per Sundnes, Gunnar 1983 http://dx.doi.org/10.1139/f83-052 http://www.nrcresearchpress.com/doi/pdf/10.1139/f83-052 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 40, issue 3, page 361-369 ISSN 0706-652X 1205-7533 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 1983 crcansciencepubl https://doi.org/10.1139/f83-052 2023-11-19T13:39:25Z We studied inter- and intra-specific variation at LDH (lactate dehydrogenase; EC 1.1.1.27) loci in 11 gadoid and 6 flatfish species regularly occurring in Norwegian coastal waters by means of isoelectric focusing and histochemical staining techniques. No two species showed identical locus A (white skeletal muscle predominating) zymograms. Polymorphism at LDH A was observed in haddock (Gadus aeglefinus), coalfish (G. virens), Norway pout (G. esmarkii), four-bearded rockling (Onos cimbrius), and dab (Limanda limanda). The allelic nature of observed LDH variants was confirmed by controlled crossings in cod (G. morhua, locus B), Norway pout (locus A), and haddock (locus A). Analyses of artificially fertilized eggs (in four species) and prenatal eggs from ripening ovaries (in 10 species) revealed a general predominance of locus A products. The maternal LDH A activity present in newly released eggs was overtaken by embryo-synthesized enzyme 1–2 d after fertilization. No unique embryonic LDH loci were observed. LDH A zymograms thus appear to be useful as a practically diagnostic tool for the identification of the pelagic fish eggs usually found in these waters. The identification method, applied to samples of pelagic eggs collected at the coast of northern Norway during March–May 1981 and 1982, showed that in a total of ~ 1500 eggs, the following species were represented: cod, Norway pout, haddock, coalfish, dab, plaice (Pleuronectes platessa), and long rough dab (Hippo-glossoides plates-soides). The species composition in these egg samples varied considerably with sampling location and sampling depth.Key words: fish egg identification, biochemical genetics, tissue enzymes, gadoids, flatfishes Article in Journal/Newspaper Northern Norway Canadian Science Publishing (via Crossref) Norway Canadian Journal of Fisheries and Aquatic Sciences 40 3 361 369 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics Mork, Jarle Solemdal, Per Sundnes, Gunnar Identification of Marine Fish Eggs: A Biochemical Genetics Approach |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
We studied inter- and intra-specific variation at LDH (lactate dehydrogenase; EC 1.1.1.27) loci in 11 gadoid and 6 flatfish species regularly occurring in Norwegian coastal waters by means of isoelectric focusing and histochemical staining techniques. No two species showed identical locus A (white skeletal muscle predominating) zymograms. Polymorphism at LDH A was observed in haddock (Gadus aeglefinus), coalfish (G. virens), Norway pout (G. esmarkii), four-bearded rockling (Onos cimbrius), and dab (Limanda limanda). The allelic nature of observed LDH variants was confirmed by controlled crossings in cod (G. morhua, locus B), Norway pout (locus A), and haddock (locus A). Analyses of artificially fertilized eggs (in four species) and prenatal eggs from ripening ovaries (in 10 species) revealed a general predominance of locus A products. The maternal LDH A activity present in newly released eggs was overtaken by embryo-synthesized enzyme 1–2 d after fertilization. No unique embryonic LDH loci were observed. LDH A zymograms thus appear to be useful as a practically diagnostic tool for the identification of the pelagic fish eggs usually found in these waters. The identification method, applied to samples of pelagic eggs collected at the coast of northern Norway during March–May 1981 and 1982, showed that in a total of ~ 1500 eggs, the following species were represented: cod, Norway pout, haddock, coalfish, dab, plaice (Pleuronectes platessa), and long rough dab (Hippo-glossoides plates-soides). The species composition in these egg samples varied considerably with sampling location and sampling depth.Key words: fish egg identification, biochemical genetics, tissue enzymes, gadoids, flatfishes |
format |
Article in Journal/Newspaper |
author |
Mork, Jarle Solemdal, Per Sundnes, Gunnar |
author_facet |
Mork, Jarle Solemdal, Per Sundnes, Gunnar |
author_sort |
Mork, Jarle |
title |
Identification of Marine Fish Eggs: A Biochemical Genetics Approach |
title_short |
Identification of Marine Fish Eggs: A Biochemical Genetics Approach |
title_full |
Identification of Marine Fish Eggs: A Biochemical Genetics Approach |
title_fullStr |
Identification of Marine Fish Eggs: A Biochemical Genetics Approach |
title_full_unstemmed |
Identification of Marine Fish Eggs: A Biochemical Genetics Approach |
title_sort |
identification of marine fish eggs: a biochemical genetics approach |
publisher |
Canadian Science Publishing |
publishDate |
1983 |
url |
http://dx.doi.org/10.1139/f83-052 http://www.nrcresearchpress.com/doi/pdf/10.1139/f83-052 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 40, issue 3, page 361-369 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f83-052 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
container_volume |
40 |
container_issue |
3 |
container_start_page |
361 |
op_container_end_page |
369 |
_version_ |
1785571364036411392 |