Viral Erythrocytic Necrosis (VEN) in Atlantic Cod ( Gadus morhua ): In Vitro Studies
The effect of natural VEN infection on cod erythrocytes in vitro was studied using several methods. It was found that VEN-infected cells were more fragile in isotonic media than uninfected cells, but there was no difference in their susceptibility to hypotonic lysis. The proportion of VEN-infected c...
| Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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| Main Authors: | , |
| Format: | Article in Journal/Newspaper |
| Language: | French |
| Published: |
Canadian Science Publishing
1980
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1139/f80-274 http://www.nrcresearchpress.com/doi/pdf/10.1139/f80-274 |
| Summary: | The effect of natural VEN infection on cod erythrocytes in vitro was studied using several methods. It was found that VEN-infected cells were more fragile in isotonic media than uninfected cells, but there was no difference in their susceptibility to hypotonic lysis. The proportion of VEN-infected cells was reduced in an inverse relationship to the time in culture. Incorporation of 3 H-labeled precursors into protein, RNA, and DNA occurred in both infected and uninfected cells during the first 6 d of incubation and declined thereafter. There was a greater incorporation of 3 H-amino acids and a tenfold greater incorporation of 3 H-thymidine into VEN-infected cells than in normal cells, while there was no difference in the incorporation of 3 H uridine by either type of cell. These results indicated that the virus of VEN was replicating in the erythrocytes and that the infection was detrimental to the cells.Key words: viral erythrocytic necrosis, diseases, Gadus morhua |
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