Quantification of Vitellogenin in Atlantic Salmon ( Salmo salar ) Plasma by Radioimmunoassay
An antibody prepared against salmon egg yolk proteins has been used to quantify Atlantic salmon (Salmo salar) plasma vitellogenin using radioimmunoassay. A low molecular weight fraction isolated from salmon egg yolk was used for radioiodination and as standard solution because plasma vitellogenin co...
Published in: | Journal of the Fisheries Research Board of Canada |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Canadian Science Publishing
1979
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Online Access: | http://dx.doi.org/10.1139/f79-082 http://www.nrcresearchpress.com/doi/pdf/10.1139/f79-082 |
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crcansciencepubl:10.1139/f79-082 2024-04-07T07:51:02+00:00 Quantification of Vitellogenin in Atlantic Salmon ( Salmo salar ) Plasma by Radioimmunoassay Idler, D. R. Hwang, S. J. Crim, L. W. 1979 http://dx.doi.org/10.1139/f79-082 http://www.nrcresearchpress.com/doi/pdf/10.1139/f79-082 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Journal of the Fisheries Research Board of Canada volume 36, issue 5, page 574-578 ISSN 0015-296X journal-article 1979 crcansciencepubl https://doi.org/10.1139/f79-082 2024-03-08T00:37:51Z An antibody prepared against salmon egg yolk proteins has been used to quantify Atlantic salmon (Salmo salar) plasma vitellogenin using radioimmunoassay. A low molecular weight fraction isolated from salmon egg yolk was used for radioiodination and as standard solution because plasma vitellogenin could not be iodinated successfully. Parallelism of the egg yolk standard to displacement given by a fraction isolated from vitellogenic salmon plasma and dilutions of plasma samples allowed the assay to be used to evaluate the state of gonadal development of migrating females several months in advance of spawning and for sexing relatively immature salmon. Key words: salmon, plasma vitellogenin; vitellogenin, quantification in salmon plasma; radioimmunoassay, salmon vitellogenin Article in Journal/Newspaper Atlantic salmon Salmo salar Canadian Science Publishing Journal of the Fisheries Research Board of Canada 36 5 574 578 |
institution |
Open Polar |
collection |
Canadian Science Publishing |
op_collection_id |
crcansciencepubl |
language |
English |
description |
An antibody prepared against salmon egg yolk proteins has been used to quantify Atlantic salmon (Salmo salar) plasma vitellogenin using radioimmunoassay. A low molecular weight fraction isolated from salmon egg yolk was used for radioiodination and as standard solution because plasma vitellogenin could not be iodinated successfully. Parallelism of the egg yolk standard to displacement given by a fraction isolated from vitellogenic salmon plasma and dilutions of plasma samples allowed the assay to be used to evaluate the state of gonadal development of migrating females several months in advance of spawning and for sexing relatively immature salmon. Key words: salmon, plasma vitellogenin; vitellogenin, quantification in salmon plasma; radioimmunoassay, salmon vitellogenin |
format |
Article in Journal/Newspaper |
author |
Idler, D. R. Hwang, S. J. Crim, L. W. |
spellingShingle |
Idler, D. R. Hwang, S. J. Crim, L. W. Quantification of Vitellogenin in Atlantic Salmon ( Salmo salar ) Plasma by Radioimmunoassay |
author_facet |
Idler, D. R. Hwang, S. J. Crim, L. W. |
author_sort |
Idler, D. R. |
title |
Quantification of Vitellogenin in Atlantic Salmon ( Salmo salar ) Plasma by Radioimmunoassay |
title_short |
Quantification of Vitellogenin in Atlantic Salmon ( Salmo salar ) Plasma by Radioimmunoassay |
title_full |
Quantification of Vitellogenin in Atlantic Salmon ( Salmo salar ) Plasma by Radioimmunoassay |
title_fullStr |
Quantification of Vitellogenin in Atlantic Salmon ( Salmo salar ) Plasma by Radioimmunoassay |
title_full_unstemmed |
Quantification of Vitellogenin in Atlantic Salmon ( Salmo salar ) Plasma by Radioimmunoassay |
title_sort |
quantification of vitellogenin in atlantic salmon ( salmo salar ) plasma by radioimmunoassay |
publisher |
Canadian Science Publishing |
publishDate |
1979 |
url |
http://dx.doi.org/10.1139/f79-082 http://www.nrcresearchpress.com/doi/pdf/10.1139/f79-082 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of the Fisheries Research Board of Canada volume 36, issue 5, page 574-578 ISSN 0015-296X |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f79-082 |
container_title |
Journal of the Fisheries Research Board of Canada |
container_volume |
36 |
container_issue |
5 |
container_start_page |
574 |
op_container_end_page |
578 |
_version_ |
1795665860158816256 |