Activation Energy and Allosteric Inhibition of Reaction Catalyzed by Aspartate Transcarbamylase from Atlantic Cod ( Gadus morhua ) Ovary
Aspartate transcarbamylase was precipitated from a 0.85% KCl extract of Atlantic cod (Gadus morhua) ovary by exhaustive dialysis. The extract contained a dialyzable, heat stable inhibitor, which when added to the reaction mixture at a concentration [Formula: see text] of that in the ovary, reduced t...
Published in: | Journal of the Fisheries Research Board of Canada |
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Canadian Science Publishing
1975
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Online Access: | http://dx.doi.org/10.1139/f75-243 http://www.nrcresearchpress.com/doi/pdf/10.1139/f75-243 |
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crcansciencepubl:10.1139/f75-243 2023-12-17T10:26:58+01:00 Activation Energy and Allosteric Inhibition of Reaction Catalyzed by Aspartate Transcarbamylase from Atlantic Cod ( Gadus morhua ) Ovary King, Deborah Baker, Dwight L. 1975 http://dx.doi.org/10.1139/f75-243 http://www.nrcresearchpress.com/doi/pdf/10.1139/f75-243 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Journal of the Fisheries Research Board of Canada volume 32, issue 11, page 2061-2064 ISSN 0015-296X General Medicine journal-article 1975 crcansciencepubl https://doi.org/10.1139/f75-243 2023-11-19T13:38:39Z Aspartate transcarbamylase was precipitated from a 0.85% KCl extract of Atlantic cod (Gadus morhua) ovary by exhaustive dialysis. The extract contained a dialyzable, heat stable inhibitor, which when added to the reaction mixture at a concentration [Formula: see text] of that in the ovary, reduced the activity to 13% and changed the aspartate concentration vs. activity curve from hyperbolic to sigmoidal. The enzyme was also inhibited by some pyrimidine derivatives but less strongly. The activation energy of the reaction catalyzed was 13,700 cal/mol and was constant between 5 and 45 C. Article in Journal/Newspaper atlantic cod Gadus morhua Canadian Science Publishing (via Crossref) Journal of the Fisheries Research Board of Canada 32 11 2061 2064 |
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Open Polar |
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Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
General Medicine |
spellingShingle |
General Medicine King, Deborah Baker, Dwight L. Activation Energy and Allosteric Inhibition of Reaction Catalyzed by Aspartate Transcarbamylase from Atlantic Cod ( Gadus morhua ) Ovary |
topic_facet |
General Medicine |
description |
Aspartate transcarbamylase was precipitated from a 0.85% KCl extract of Atlantic cod (Gadus morhua) ovary by exhaustive dialysis. The extract contained a dialyzable, heat stable inhibitor, which when added to the reaction mixture at a concentration [Formula: see text] of that in the ovary, reduced the activity to 13% and changed the aspartate concentration vs. activity curve from hyperbolic to sigmoidal. The enzyme was also inhibited by some pyrimidine derivatives but less strongly. The activation energy of the reaction catalyzed was 13,700 cal/mol and was constant between 5 and 45 C. |
format |
Article in Journal/Newspaper |
author |
King, Deborah Baker, Dwight L. |
author_facet |
King, Deborah Baker, Dwight L. |
author_sort |
King, Deborah |
title |
Activation Energy and Allosteric Inhibition of Reaction Catalyzed by Aspartate Transcarbamylase from Atlantic Cod ( Gadus morhua ) Ovary |
title_short |
Activation Energy and Allosteric Inhibition of Reaction Catalyzed by Aspartate Transcarbamylase from Atlantic Cod ( Gadus morhua ) Ovary |
title_full |
Activation Energy and Allosteric Inhibition of Reaction Catalyzed by Aspartate Transcarbamylase from Atlantic Cod ( Gadus morhua ) Ovary |
title_fullStr |
Activation Energy and Allosteric Inhibition of Reaction Catalyzed by Aspartate Transcarbamylase from Atlantic Cod ( Gadus morhua ) Ovary |
title_full_unstemmed |
Activation Energy and Allosteric Inhibition of Reaction Catalyzed by Aspartate Transcarbamylase from Atlantic Cod ( Gadus morhua ) Ovary |
title_sort |
activation energy and allosteric inhibition of reaction catalyzed by aspartate transcarbamylase from atlantic cod ( gadus morhua ) ovary |
publisher |
Canadian Science Publishing |
publishDate |
1975 |
url |
http://dx.doi.org/10.1139/f75-243 http://www.nrcresearchpress.com/doi/pdf/10.1139/f75-243 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Journal of the Fisheries Research Board of Canada volume 32, issue 11, page 2061-2064 ISSN 0015-296X |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f75-243 |
container_title |
Journal of the Fisheries Research Board of Canada |
container_volume |
32 |
container_issue |
11 |
container_start_page |
2061 |
op_container_end_page |
2064 |
_version_ |
1785578726993428480 |