Effects of Formaldehyde on Salt Extractable Proteins of Gadoid Muscle

• Published in: Journal of the Fisheries Research Board of Canada
• Main Authors: Castell, C. H., Smith, B., Dyer, W. J.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Canadian Science Publishing 1973
• Subjects: General Medicine; Hake; atlantic cod; Gadus morhua
• Online Access: http://dx.doi.org/10.1139/f73-191; http://www.nrcresearchpress.com/doi/pdf/10.1139/f73-191

Addition of formaldehyde to fresh cod muscle, to give concentrations of 10 to 200 ppm, brought about marked decreases in the extractable protein content during holding periods of 24 hr or less at 0 C. Similar levels of formaldehyde, produced during frozen storage of gadoid (Atlantic cod, Gadus morhua, pollock, Pollachius virens, cusk, Brosme brosme, and silver hake, Merluccius bilinearis), fillets at −5 C, brought about similar reductions in the extractable proteins. Comparative tests showed that, in the concentrations normally encountered in deteriorating frozen gadoid fillets, formaldehyde was a much more active protein-insolubilizing agent than free fatty acid. It is evident that in these protein changes more than one mechanism is involved. Observed species-differences in the extent to which fish proteins became insolubilized during storage appeared to be related to presence or absence of these different mechanisms. The more rapid and more extensive denaturation of most gadoid fillets in frozen storage than of fillets of nongadoid species appears to be directly related to the presence of muscle enzyme of the former group that is capable of producing formaldehyde from trimethylamine oxide, which is absent in the muscle of the nongadoid species so far tested.