Cryogenic Preservation of Atlantic Cod ( Gadus Morhua ) Sperm
Cryogenic preservation of cod sperm, with retention of fertilizing capacity, was achieved. Sperm were deep frozen to −79 C or −196 C in the presence of protecting agents. Of the conditions tested, most satisfactory freezing was accomplished in a mixture containing 1 part semen and 3 parts of a mediu...
| Published in: | Journal of the Fisheries Research Board of Canada |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Canadian Science Publishing
1968
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1139/f68-232 http://www.nrcresearchpress.com/doi/pdf/10.1139/f68-232 |
| Summary: | Cryogenic preservation of cod sperm, with retention of fertilizing capacity, was achieved. Sperm were deep frozen to −79 C or −196 C in the presence of protecting agents. Of the conditions tested, most satisfactory freezing was accomplished in a mixture containing 1 part semen and 3 parts of a medium that consisted of 40 parts 0.4 M NaCl − 0.1 M glycine: 8 parts of 1.3% sodium bicarbonate: 10 parts of glycerol. A freezing rate of 5 degrees C/min was more successful than one of 1 degree C/min.When frozen sperm were stored at −196 C for periods up to 60 days the average fertility was 36 ± 12% (seven samples) compared with 79 ± 6% (seven samples) for fresh sperm on the same batch of eggs. Successful storage of cod sperm at −79 C for a year was achieved. |
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