A method to bin alleles of genetic loci that maintains population heterogeneity
Fishery resources are often studied when individuals from multiple stocks are aggregated. For that reason, mixed-stock analysis (MSA), i.e., estimation of the stock composition of a mixture of individuals, is an important component of many research programs. Although many characteristics can be used...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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Online Access: | http://dx.doi.org/10.1139/f05-093 http://www.nrcresearchpress.com/doi/pdf/10.1139/f05-093 |
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crcansciencepubl:10.1139/f05-093 2023-12-17T10:51:32+01:00 A method to bin alleles of genetic loci that maintains population heterogeneity Bromaghin, Jeffrey F Crane, Penelope A 2005 http://dx.doi.org/10.1139/f05-093 http://www.nrcresearchpress.com/doi/pdf/10.1139/f05-093 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 62, issue 7, page 1570-1579 ISSN 0706-652X 1205-7533 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 2005 crcansciencepubl https://doi.org/10.1139/f05-093 2023-11-19T13:38:17Z Fishery resources are often studied when individuals from multiple stocks are aggregated. For that reason, mixed-stock analysis (MSA), i.e., estimation of the stock composition of a mixture of individuals, is an important component of many research programs. Although many characteristics can be used in MSA, DNA loci, particularly microsatellites, have become extremely common. Microsatellite loci usually have a greater number of potential expressions, or alleles, than other marker types. A high degree of polymorphism can enhance the power of MSA, but allele proportions are estimated less precisely and rare alleles are absent or observed in very small numbers in typically sized samples. The reduced precision and presence of rare alleles can degrade the performance of some analytic methods. Although the effect can be reduced by binning alleles, which is common, an objective method of doing so has not been available previously. We present a method for binning alleles that reduces the number of rare alleles, largely preserves the genetic relationships observed among stocks, and modestly improves the performance of mixed-stock and individual-assignment analyses. The method is illustrated with data from Yukon River chum salmon (Oncorhynchus keta) and western Alaska Dolly Varden (Salvelinus malma). Article in Journal/Newspaper Yukon river Alaska Yukon Canadian Science Publishing (via Crossref) Yukon Keta ENVELOPE(-19.455,-19.455,65.656,65.656) Varden ENVELOPE(7.656,7.656,62.534,62.534) Canadian Journal of Fisheries and Aquatic Sciences 62 7 1570 1579 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics Bromaghin, Jeffrey F Crane, Penelope A A method to bin alleles of genetic loci that maintains population heterogeneity |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
Fishery resources are often studied when individuals from multiple stocks are aggregated. For that reason, mixed-stock analysis (MSA), i.e., estimation of the stock composition of a mixture of individuals, is an important component of many research programs. Although many characteristics can be used in MSA, DNA loci, particularly microsatellites, have become extremely common. Microsatellite loci usually have a greater number of potential expressions, or alleles, than other marker types. A high degree of polymorphism can enhance the power of MSA, but allele proportions are estimated less precisely and rare alleles are absent or observed in very small numbers in typically sized samples. The reduced precision and presence of rare alleles can degrade the performance of some analytic methods. Although the effect can be reduced by binning alleles, which is common, an objective method of doing so has not been available previously. We present a method for binning alleles that reduces the number of rare alleles, largely preserves the genetic relationships observed among stocks, and modestly improves the performance of mixed-stock and individual-assignment analyses. The method is illustrated with data from Yukon River chum salmon (Oncorhynchus keta) and western Alaska Dolly Varden (Salvelinus malma). |
format |
Article in Journal/Newspaper |
author |
Bromaghin, Jeffrey F Crane, Penelope A |
author_facet |
Bromaghin, Jeffrey F Crane, Penelope A |
author_sort |
Bromaghin, Jeffrey F |
title |
A method to bin alleles of genetic loci that maintains population heterogeneity |
title_short |
A method to bin alleles of genetic loci that maintains population heterogeneity |
title_full |
A method to bin alleles of genetic loci that maintains population heterogeneity |
title_fullStr |
A method to bin alleles of genetic loci that maintains population heterogeneity |
title_full_unstemmed |
A method to bin alleles of genetic loci that maintains population heterogeneity |
title_sort |
method to bin alleles of genetic loci that maintains population heterogeneity |
publisher |
Canadian Science Publishing |
publishDate |
2005 |
url |
http://dx.doi.org/10.1139/f05-093 http://www.nrcresearchpress.com/doi/pdf/10.1139/f05-093 |
long_lat |
ENVELOPE(-19.455,-19.455,65.656,65.656) ENVELOPE(7.656,7.656,62.534,62.534) |
geographic |
Yukon Keta Varden |
geographic_facet |
Yukon Keta Varden |
genre |
Yukon river Alaska Yukon |
genre_facet |
Yukon river Alaska Yukon |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 62, issue 7, page 1570-1579 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f05-093 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
container_volume |
62 |
container_issue |
7 |
container_start_page |
1570 |
op_container_end_page |
1579 |
_version_ |
1785576822159704064 |