Definitive identification of fatty acid constituents in marine mammal tissues
Analyses of fatty acid constituents in a sample of bowhead whale (Balaena mysticetus) blubber were conducted using a method that has not been applied before to studies of lipids in marine mammals. It involves creation and analysis of nitrogen (picolinyl) ester derivatives of the fatty acids followed...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Canadian Science Publishing
2004
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1139/f04-021 http://www.nrcresearchpress.com/doi/pdf/10.1139/f04-021 |
id |
crcansciencepubl:10.1139/f04-021 |
---|---|
record_format |
openpolar |
spelling |
crcansciencepubl:10.1139/f04-021 2023-12-17T10:27:39+01:00 Definitive identification of fatty acid constituents in marine mammal tissues Wetzel, D L Reynolds III, J E 2004 http://dx.doi.org/10.1139/f04-021 http://www.nrcresearchpress.com/doi/pdf/10.1139/f04-021 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 61, issue 4, page 554-560 ISSN 0706-652X 1205-7533 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 2004 crcansciencepubl https://doi.org/10.1139/f04-021 2023-11-19T13:39:13Z Analyses of fatty acid constituents in a sample of bowhead whale (Balaena mysticetus) blubber were conducted using a method that has not been applied before to studies of lipids in marine mammals. It involves creation and analysis of nitrogen (picolinyl) ester derivatives of the fatty acids followed by combined gas chromatography mass spectrometry for verification of structures. Use of this approach allowed the structural confirmation of 45 different fatty acids in the blubber sample. The traditional method of methyl ester derivatization of fatty acids followed by gas chromatography mass spectrometry analyses provides more component characterization than analyses by gas chromatography flame ionization detection, but not enough to effectively differentiate double bond isomers or branched compounds. Although the novel approach is time intensive, we recommend that it be employed for studies where the precise identification and confirmation of fatty acids is important. Article in Journal/Newspaper Balaena mysticetus bowhead whale Canadian Science Publishing (via Crossref) Canadian Journal of Fisheries and Aquatic Sciences 61 4 554 560 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics Wetzel, D L Reynolds III, J E Definitive identification of fatty acid constituents in marine mammal tissues |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
Analyses of fatty acid constituents in a sample of bowhead whale (Balaena mysticetus) blubber were conducted using a method that has not been applied before to studies of lipids in marine mammals. It involves creation and analysis of nitrogen (picolinyl) ester derivatives of the fatty acids followed by combined gas chromatography mass spectrometry for verification of structures. Use of this approach allowed the structural confirmation of 45 different fatty acids in the blubber sample. The traditional method of methyl ester derivatization of fatty acids followed by gas chromatography mass spectrometry analyses provides more component characterization than analyses by gas chromatography flame ionization detection, but not enough to effectively differentiate double bond isomers or branched compounds. Although the novel approach is time intensive, we recommend that it be employed for studies where the precise identification and confirmation of fatty acids is important. |
format |
Article in Journal/Newspaper |
author |
Wetzel, D L Reynolds III, J E |
author_facet |
Wetzel, D L Reynolds III, J E |
author_sort |
Wetzel, D L |
title |
Definitive identification of fatty acid constituents in marine mammal tissues |
title_short |
Definitive identification of fatty acid constituents in marine mammal tissues |
title_full |
Definitive identification of fatty acid constituents in marine mammal tissues |
title_fullStr |
Definitive identification of fatty acid constituents in marine mammal tissues |
title_full_unstemmed |
Definitive identification of fatty acid constituents in marine mammal tissues |
title_sort |
definitive identification of fatty acid constituents in marine mammal tissues |
publisher |
Canadian Science Publishing |
publishDate |
2004 |
url |
http://dx.doi.org/10.1139/f04-021 http://www.nrcresearchpress.com/doi/pdf/10.1139/f04-021 |
genre |
Balaena mysticetus bowhead whale |
genre_facet |
Balaena mysticetus bowhead whale |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 61, issue 4, page 554-560 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f04-021 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
container_volume |
61 |
container_issue |
4 |
container_start_page |
554 |
op_container_end_page |
560 |
_version_ |
1785579567825551360 |