Osmoregulatory plasticity of the glass eel of Anguilla anguilla : freshwater entry and changes in branchial ion-transport protein expression
The glass-eel stage of the life history of Anguillid eels (Anguilla spp.) makes the important physiological transition from seawater (SW) to freshwater (FW) osmoregulation. Central to successful adaptation to fresh water is the switch from active hypoosmoregulation (ion excretion) to active hyperosm...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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Online Access: | http://dx.doi.org/10.1139/f04-010 http://www.nrcresearchpress.com/doi/pdf/10.1139/f04-010 |
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crcansciencepubl:10.1139/f04-010 2023-12-17T10:18:30+01:00 Osmoregulatory plasticity of the glass eel of Anguilla anguilla : freshwater entry and changes in branchial ion-transport protein expression Wilson, Jonathan Mark Antunes, José Carlos Bouça, Patricío Duro Coimbra, João 2004 http://dx.doi.org/10.1139/f04-010 http://www.nrcresearchpress.com/doi/pdf/10.1139/f04-010 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 61, issue 3, page 432-442 ISSN 0706-652X 1205-7533 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 2004 crcansciencepubl https://doi.org/10.1139/f04-010 2023-11-19T13:39:32Z The glass-eel stage of the life history of Anguillid eels (Anguilla spp.) makes the important physiological transition from seawater (SW) to freshwater (FW) osmoregulation. Central to successful adaptation to fresh water is the switch from active hypoosmoregulation (ion excretion) to active hyperosmoregulation (ion uptake) to counter passive fluxes imposed by concentration gradients. Anguilla anguilla (L.) glass eels, captured from the lower Minho River estuary and maintained in brackish water (BW; 24), were fully capable of adapting to FW following acute transfer. In a comparison between SW- (34) and FW-adapted glass eels, gill Na + /K + -ATPase and Na + :K + :2Cl cotransporter (NKCC) expression were both markedly reduced in the FW group. Branchial mitochondria-rich chloride cells (MRCs) were also significantly larger in SW-adapted glass eels. Apical CFTR Cl channel immunoreactivity associated with branchial MRCs was also only present in SW-adapted glass eels. The expression of these three ion-transport proteins is strongly associated with active ion excretion in SW teleost fishes. In FW-adapted glass eels, cortisol treatment increased branchial Na + /K + -ATPase expression but was without effect on NKCC expression. Glucocorticoid receptor blockade by RU-486 had no effect on Na + /K + -ATPase or NKCC expression. Article in Journal/Newspaper Anguilla anguilla Canadian Science Publishing (via Crossref) Canadian Journal of Fisheries and Aquatic Sciences 61 3 432 442 |
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Open Polar |
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Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
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Aquatic Science Ecology, Evolution, Behavior and Systematics Wilson, Jonathan Mark Antunes, José Carlos Bouça, Patricío Duro Coimbra, João Osmoregulatory plasticity of the glass eel of Anguilla anguilla : freshwater entry and changes in branchial ion-transport protein expression |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
The glass-eel stage of the life history of Anguillid eels (Anguilla spp.) makes the important physiological transition from seawater (SW) to freshwater (FW) osmoregulation. Central to successful adaptation to fresh water is the switch from active hypoosmoregulation (ion excretion) to active hyperosmoregulation (ion uptake) to counter passive fluxes imposed by concentration gradients. Anguilla anguilla (L.) glass eels, captured from the lower Minho River estuary and maintained in brackish water (BW; 24), were fully capable of adapting to FW following acute transfer. In a comparison between SW- (34) and FW-adapted glass eels, gill Na + /K + -ATPase and Na + :K + :2Cl cotransporter (NKCC) expression were both markedly reduced in the FW group. Branchial mitochondria-rich chloride cells (MRCs) were also significantly larger in SW-adapted glass eels. Apical CFTR Cl channel immunoreactivity associated with branchial MRCs was also only present in SW-adapted glass eels. The expression of these three ion-transport proteins is strongly associated with active ion excretion in SW teleost fishes. In FW-adapted glass eels, cortisol treatment increased branchial Na + /K + -ATPase expression but was without effect on NKCC expression. Glucocorticoid receptor blockade by RU-486 had no effect on Na + /K + -ATPase or NKCC expression. |
format |
Article in Journal/Newspaper |
author |
Wilson, Jonathan Mark Antunes, José Carlos Bouça, Patricío Duro Coimbra, João |
author_facet |
Wilson, Jonathan Mark Antunes, José Carlos Bouça, Patricío Duro Coimbra, João |
author_sort |
Wilson, Jonathan Mark |
title |
Osmoregulatory plasticity of the glass eel of Anguilla anguilla : freshwater entry and changes in branchial ion-transport protein expression |
title_short |
Osmoregulatory plasticity of the glass eel of Anguilla anguilla : freshwater entry and changes in branchial ion-transport protein expression |
title_full |
Osmoregulatory plasticity of the glass eel of Anguilla anguilla : freshwater entry and changes in branchial ion-transport protein expression |
title_fullStr |
Osmoregulatory plasticity of the glass eel of Anguilla anguilla : freshwater entry and changes in branchial ion-transport protein expression |
title_full_unstemmed |
Osmoregulatory plasticity of the glass eel of Anguilla anguilla : freshwater entry and changes in branchial ion-transport protein expression |
title_sort |
osmoregulatory plasticity of the glass eel of anguilla anguilla : freshwater entry and changes in branchial ion-transport protein expression |
publisher |
Canadian Science Publishing |
publishDate |
2004 |
url |
http://dx.doi.org/10.1139/f04-010 http://www.nrcresearchpress.com/doi/pdf/10.1139/f04-010 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 61, issue 3, page 432-442 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f04-010 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
container_volume |
61 |
container_issue |
3 |
container_start_page |
432 |
op_container_end_page |
442 |
_version_ |
1785572805520130048 |