The effects of environmental heat stress on heat-shock mRNA and protein expression in Miramichi Atlantic salmon ( Salmo salar) parr
This study combines laboratory experiments with temperature monitoring and fish sampling in the wild to determine if Atlantic salmon (Salmo salar) parr from the Miramichi River in New Brunswick are currently experiencing significant sublethal heat stress during the warm summer months. Laboratory exp...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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Online Access: | http://dx.doi.org/10.1139/f02-117 http://www.nrcresearchpress.com/doi/pdf/10.1139/f02-117 |
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crcansciencepubl:10.1139/f02-117 2024-06-23T07:51:11+00:00 The effects of environmental heat stress on heat-shock mRNA and protein expression in Miramichi Atlantic salmon ( Salmo salar) parr Lund, Susan G Caissie, Daniel Cunjak, Richard A Vijayan, Mathilakath M Tufts, Bruce L 2002 http://dx.doi.org/10.1139/f02-117 http://www.nrcresearchpress.com/doi/pdf/10.1139/f02-117 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 59, issue 9, page 1553-1562 ISSN 0706-652X 1205-7533 journal-article 2002 crcansciencepubl https://doi.org/10.1139/f02-117 2024-05-30T08:13:50Z This study combines laboratory experiments with temperature monitoring and fish sampling in the wild to determine if Atlantic salmon (Salmo salar) parr from the Miramichi River in New Brunswick are currently experiencing significant sublethal heat stress during the warm summer months. Laboratory experiments indicated that Hsp 70 mRNA and protein and Hsp 30 mRNA were all significantly induced in Atlantic salmon parr between 22°C and 25°C. Field sampling during moderate spring temperatures and a high-temperature event in summer further indicated that the threshold for mRNA induction of both Hsp 70 and Hsp 30 is around 23°C, but Hsp 70 protein levels were only significantly elevated in the field at 27°C. Hsc 70 mRNA and protein levels were not significantly increased during heat stress under laboratory conditions. In the field, however, Hsc 70 mRNA was significantly increased at 23°C and both Hsc 70 mRNA and protein levels were elevated at 27°C. Taken together, the results of this investigation suggest that Atlantic salmon parr from the Miramichi River are currently experiencing temperatures that will cause significant protein damage and induce a heat-shock response for about 30 days each summer. Article in Journal/Newspaper Atlantic salmon Salmo salar Canadian Science Publishing Canadian Journal of Fisheries and Aquatic Sciences 59 9 1553 1562 |
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Open Polar |
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Canadian Science Publishing |
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crcansciencepubl |
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English |
description |
This study combines laboratory experiments with temperature monitoring and fish sampling in the wild to determine if Atlantic salmon (Salmo salar) parr from the Miramichi River in New Brunswick are currently experiencing significant sublethal heat stress during the warm summer months. Laboratory experiments indicated that Hsp 70 mRNA and protein and Hsp 30 mRNA were all significantly induced in Atlantic salmon parr between 22°C and 25°C. Field sampling during moderate spring temperatures and a high-temperature event in summer further indicated that the threshold for mRNA induction of both Hsp 70 and Hsp 30 is around 23°C, but Hsp 70 protein levels were only significantly elevated in the field at 27°C. Hsc 70 mRNA and protein levels were not significantly increased during heat stress under laboratory conditions. In the field, however, Hsc 70 mRNA was significantly increased at 23°C and both Hsc 70 mRNA and protein levels were elevated at 27°C. Taken together, the results of this investigation suggest that Atlantic salmon parr from the Miramichi River are currently experiencing temperatures that will cause significant protein damage and induce a heat-shock response for about 30 days each summer. |
format |
Article in Journal/Newspaper |
author |
Lund, Susan G Caissie, Daniel Cunjak, Richard A Vijayan, Mathilakath M Tufts, Bruce L |
spellingShingle |
Lund, Susan G Caissie, Daniel Cunjak, Richard A Vijayan, Mathilakath M Tufts, Bruce L The effects of environmental heat stress on heat-shock mRNA and protein expression in Miramichi Atlantic salmon ( Salmo salar) parr |
author_facet |
Lund, Susan G Caissie, Daniel Cunjak, Richard A Vijayan, Mathilakath M Tufts, Bruce L |
author_sort |
Lund, Susan G |
title |
The effects of environmental heat stress on heat-shock mRNA and protein expression in Miramichi Atlantic salmon ( Salmo salar) parr |
title_short |
The effects of environmental heat stress on heat-shock mRNA and protein expression in Miramichi Atlantic salmon ( Salmo salar) parr |
title_full |
The effects of environmental heat stress on heat-shock mRNA and protein expression in Miramichi Atlantic salmon ( Salmo salar) parr |
title_fullStr |
The effects of environmental heat stress on heat-shock mRNA and protein expression in Miramichi Atlantic salmon ( Salmo salar) parr |
title_full_unstemmed |
The effects of environmental heat stress on heat-shock mRNA and protein expression in Miramichi Atlantic salmon ( Salmo salar) parr |
title_sort |
effects of environmental heat stress on heat-shock mrna and protein expression in miramichi atlantic salmon ( salmo salar) parr |
publisher |
Canadian Science Publishing |
publishDate |
2002 |
url |
http://dx.doi.org/10.1139/f02-117 http://www.nrcresearchpress.com/doi/pdf/10.1139/f02-117 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 59, issue 9, page 1553-1562 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/f02-117 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
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59 |
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9 |
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1553 |
op_container_end_page |
1562 |
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1802642208359186432 |