Enterococcus species diversity in fecal samples of wild marine species as determined by real-time PCR
Analyses using culture-independent molecular techniques have improved our understanding of microbial composition. The aim of this work was to identify and quantify enterococci in fecal samples of wild marine species using real-time quantitative PCR. Seven Enterococcus species were examined in fecal...
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crcansciencepubl:10.1139/cjm-2016-0427 2023-12-17T10:28:29+01:00 Enterococcus species diversity in fecal samples of wild marine species as determined by real-time PCR Medeiros, Aline Weber Blaese Amorim, Derek Tavares, Maurício de Moura, Tiane Martin Franco, Ana Claudia d’Azevedo, Pedro Alves Frazzon, Jeverson Frazzon, Ana Paula Guedes 2017 http://dx.doi.org/10.1139/cjm-2016-0427 http://www.nrcresearchpress.com/doi/full-xml/10.1139/cjm-2016-0427 http://www.nrcresearchpress.com/doi/pdf/10.1139/cjm-2016-0427 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 63, issue 2, page 129-136 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 2017 crcansciencepubl https://doi.org/10.1139/cjm-2016-0427 2023-11-19T13:39:26Z Analyses using culture-independent molecular techniques have improved our understanding of microbial composition. The aim of this work was to identify and quantify enterococci in fecal samples of wild marine species using real-time quantitative PCR. Seven Enterococcus species were examined in fecal DNA of South American fur seals (Arctocephalus australis), Subantarctic fur seals (Arctocephalus tropicalis), green turtles (Chelonia mydas), Magellanic penguins (Spheniscus magellanicus), snowy-crowned tern (Sterna trudeaui), white-backed stilt (Himantopus melanurus), white-chinned petrels (Procellaria aequinoctialis), red knot (Calidris canutus), and black-browed albatross (Thalassarche melanophris). All Enterococcus species evaluated were detected in all fecal samples of wild marine species, with a concentration ranging between 10 6 and 10 12 copies/ng of total DNA. Differences in the enterococci distribution were observed. Enterococcus faecalis and Enterococcus mundtii were most abundant in marine mammals. Enterococcus faecalis was frequent in green turtle, Magellanic penguin, snowy-crowned tern, red knot, and black-browed albatross. Enterococcus hirae and Enterococcus gallinarum showed elevated occurrence in white-backed stilt, and Enterococcus faecium in white-chinned petrel. This study showed highest diversity of enterococci in feces of wild marine species than currently available data, and reinforced the use of culture-independent analysis to help us to enhance our understanding of enterococci in gastrointestinal tracts of wild marine species. Article in Journal/Newspaper Calidris canutus Red Knot Canadian Science Publishing (via Crossref) Canadian Journal of Microbiology 63 2 129 136 |
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Open Polar |
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Canadian Science Publishing (via Crossref) |
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crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
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Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Medeiros, Aline Weber Blaese Amorim, Derek Tavares, Maurício de Moura, Tiane Martin Franco, Ana Claudia d’Azevedo, Pedro Alves Frazzon, Jeverson Frazzon, Ana Paula Guedes Enterococcus species diversity in fecal samples of wild marine species as determined by real-time PCR |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
Analyses using culture-independent molecular techniques have improved our understanding of microbial composition. The aim of this work was to identify and quantify enterococci in fecal samples of wild marine species using real-time quantitative PCR. Seven Enterococcus species were examined in fecal DNA of South American fur seals (Arctocephalus australis), Subantarctic fur seals (Arctocephalus tropicalis), green turtles (Chelonia mydas), Magellanic penguins (Spheniscus magellanicus), snowy-crowned tern (Sterna trudeaui), white-backed stilt (Himantopus melanurus), white-chinned petrels (Procellaria aequinoctialis), red knot (Calidris canutus), and black-browed albatross (Thalassarche melanophris). All Enterococcus species evaluated were detected in all fecal samples of wild marine species, with a concentration ranging between 10 6 and 10 12 copies/ng of total DNA. Differences in the enterococci distribution were observed. Enterococcus faecalis and Enterococcus mundtii were most abundant in marine mammals. Enterococcus faecalis was frequent in green turtle, Magellanic penguin, snowy-crowned tern, red knot, and black-browed albatross. Enterococcus hirae and Enterococcus gallinarum showed elevated occurrence in white-backed stilt, and Enterococcus faecium in white-chinned petrel. This study showed highest diversity of enterococci in feces of wild marine species than currently available data, and reinforced the use of culture-independent analysis to help us to enhance our understanding of enterococci in gastrointestinal tracts of wild marine species. |
format |
Article in Journal/Newspaper |
author |
Medeiros, Aline Weber Blaese Amorim, Derek Tavares, Maurício de Moura, Tiane Martin Franco, Ana Claudia d’Azevedo, Pedro Alves Frazzon, Jeverson Frazzon, Ana Paula Guedes |
author_facet |
Medeiros, Aline Weber Blaese Amorim, Derek Tavares, Maurício de Moura, Tiane Martin Franco, Ana Claudia d’Azevedo, Pedro Alves Frazzon, Jeverson Frazzon, Ana Paula Guedes |
author_sort |
Medeiros, Aline Weber |
title |
Enterococcus species diversity in fecal samples of wild marine species as determined by real-time PCR |
title_short |
Enterococcus species diversity in fecal samples of wild marine species as determined by real-time PCR |
title_full |
Enterococcus species diversity in fecal samples of wild marine species as determined by real-time PCR |
title_fullStr |
Enterococcus species diversity in fecal samples of wild marine species as determined by real-time PCR |
title_full_unstemmed |
Enterococcus species diversity in fecal samples of wild marine species as determined by real-time PCR |
title_sort |
enterococcus species diversity in fecal samples of wild marine species as determined by real-time pcr |
publisher |
Canadian Science Publishing |
publishDate |
2017 |
url |
http://dx.doi.org/10.1139/cjm-2016-0427 http://www.nrcresearchpress.com/doi/full-xml/10.1139/cjm-2016-0427 http://www.nrcresearchpress.com/doi/pdf/10.1139/cjm-2016-0427 |
genre |
Calidris canutus Red Knot |
genre_facet |
Calidris canutus Red Knot |
op_source |
Canadian Journal of Microbiology volume 63, issue 2, page 129-136 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/cjm-2016-0427 |
container_title |
Canadian Journal of Microbiology |
container_volume |
63 |
container_issue |
2 |
container_start_page |
129 |
op_container_end_page |
136 |
_version_ |
1785580611500507136 |