Improving diversity in cultures of bacteria from an extreme environment
The ikaite columns in the Ikka Fjord in Greenland represent one of the few permanently cold and alkaline environments on Earth, and the interior of the columns is home to a bacterial community adapted to these extreme conditions. The community is characterized by low cell numbers imbedded in a calci...
Published in: | Canadian Journal of Microbiology |
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crcansciencepubl:10.1139/cjm-2013-0087 2023-12-17T10:31:01+01:00 Improving diversity in cultures of bacteria from an extreme environment Vester, Jan Kjølhede Glaring, Mikkel Andreas Stougaard, Peter 2013 http://dx.doi.org/10.1139/cjm-2013-0087 http://www.nrcresearchpress.com/doi/full-xml/10.1139/cjm-2013-0087 http://www.nrcresearchpress.com/doi/pdf/10.1139/cjm-2013-0087 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Microbiology volume 59, issue 8, page 581-586 ISSN 0008-4166 1480-3275 Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology journal-article 2013 crcansciencepubl https://doi.org/10.1139/cjm-2013-0087 2023-11-19T13:39:06Z The ikaite columns in the Ikka Fjord in Greenland represent one of the few permanently cold and alkaline environments on Earth, and the interior of the columns is home to a bacterial community adapted to these extreme conditions. The community is characterized by low cell numbers imbedded in a calcium carbonate matrix, making extraction of bacterial cells and DNA a challenge and limiting molecular and genomic studies of this environment. To utilize this genetic resource, cultivation at high pH and low temperature was studied as a method for obtaining biomass and DNA from the fraction of this community that would not otherwise be amenable to genetic analyses. The diversity and community dynamics in mixed cultures of bacteria from ikaite columns was investigated using denaturing gradient gel electrophoresis and pyrosequencing of 16S rDNA. Both medium composition and incubation time influenced the diversity of the culture and many hitherto uncharacterized genera could be brought into culture by extended incubation time. Extended incubation time also gave rise to a more diverse community with a significant number of rare species not detected in the initial community. Article in Journal/Newspaper Greenland Canadian Science Publishing (via Crossref) Greenland Ikka ENVELOPE(-48.100,-48.100,61.150,61.150) Canadian Journal of Microbiology 59 8 581 586 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
spellingShingle |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology Vester, Jan Kjølhede Glaring, Mikkel Andreas Stougaard, Peter Improving diversity in cultures of bacteria from an extreme environment |
topic_facet |
Genetics Molecular Biology Applied Microbiology and Biotechnology General Medicine Immunology Microbiology |
description |
The ikaite columns in the Ikka Fjord in Greenland represent one of the few permanently cold and alkaline environments on Earth, and the interior of the columns is home to a bacterial community adapted to these extreme conditions. The community is characterized by low cell numbers imbedded in a calcium carbonate matrix, making extraction of bacterial cells and DNA a challenge and limiting molecular and genomic studies of this environment. To utilize this genetic resource, cultivation at high pH and low temperature was studied as a method for obtaining biomass and DNA from the fraction of this community that would not otherwise be amenable to genetic analyses. The diversity and community dynamics in mixed cultures of bacteria from ikaite columns was investigated using denaturing gradient gel electrophoresis and pyrosequencing of 16S rDNA. Both medium composition and incubation time influenced the diversity of the culture and many hitherto uncharacterized genera could be brought into culture by extended incubation time. Extended incubation time also gave rise to a more diverse community with a significant number of rare species not detected in the initial community. |
format |
Article in Journal/Newspaper |
author |
Vester, Jan Kjølhede Glaring, Mikkel Andreas Stougaard, Peter |
author_facet |
Vester, Jan Kjølhede Glaring, Mikkel Andreas Stougaard, Peter |
author_sort |
Vester, Jan Kjølhede |
title |
Improving diversity in cultures of bacteria from an extreme environment |
title_short |
Improving diversity in cultures of bacteria from an extreme environment |
title_full |
Improving diversity in cultures of bacteria from an extreme environment |
title_fullStr |
Improving diversity in cultures of bacteria from an extreme environment |
title_full_unstemmed |
Improving diversity in cultures of bacteria from an extreme environment |
title_sort |
improving diversity in cultures of bacteria from an extreme environment |
publisher |
Canadian Science Publishing |
publishDate |
2013 |
url |
http://dx.doi.org/10.1139/cjm-2013-0087 http://www.nrcresearchpress.com/doi/full-xml/10.1139/cjm-2013-0087 http://www.nrcresearchpress.com/doi/pdf/10.1139/cjm-2013-0087 |
long_lat |
ENVELOPE(-48.100,-48.100,61.150,61.150) |
geographic |
Greenland Ikka |
geographic_facet |
Greenland Ikka |
genre |
Greenland |
genre_facet |
Greenland |
op_source |
Canadian Journal of Microbiology volume 59, issue 8, page 581-586 ISSN 0008-4166 1480-3275 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/cjm-2013-0087 |
container_title |
Canadian Journal of Microbiology |
container_volume |
59 |
container_issue |
8 |
container_start_page |
581 |
op_container_end_page |
586 |
_version_ |
1785584153393102848 |