Subalpine fir microsatellite variation reveals the complex relationship between var. lasiocarpa and var. bifolia
Subalpine fir, Abies lasiocarpa, occurs throughout western North America, often in forest–tundra parkland. To resolve the presence of varieties in this species, we surveyed microsatellite genetic markers in 11 populations containing three putative varieties of Abies lasiocarpa: (1) var. lasiocarpa,...
Published in: | Canadian Journal of Forest Research |
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2022
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Online Access: | http://dx.doi.org/10.1139/cjfr-2021-0303 https://cdnsciencepub.com/doi/full-xml/10.1139/cjfr-2021-0303 https://cdnsciencepub.com/doi/pdf/10.1139/cjfr-2021-0303 |
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crcansciencepubl:10.1139/cjfr-2021-0303 2023-12-17T10:51:13+01:00 Subalpine fir microsatellite variation reveals the complex relationship between var. lasiocarpa and var. bifolia Ritland, Kermit Miscampbell, Allyson Cartwright, Charlie Bohlmann, Nikhil Ritland, Carol Natural Resources and Engineering Research Council of Canada Ministry of Forests, Lands, Natural Resource Operations and Rural Development 2022 http://dx.doi.org/10.1139/cjfr-2021-0303 https://cdnsciencepub.com/doi/full-xml/10.1139/cjfr-2021-0303 https://cdnsciencepub.com/doi/pdf/10.1139/cjfr-2021-0303 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Forest Research volume 52, issue 6, page 901-909 ISSN 0045-5067 1208-6037 Ecology Forestry Global and Planetary Change journal-article 2022 crcansciencepubl https://doi.org/10.1139/cjfr-2021-0303 2023-11-19T13:38:42Z Subalpine fir, Abies lasiocarpa, occurs throughout western North America, often in forest–tundra parkland. To resolve the presence of varieties in this species, we surveyed microsatellite genetic markers in 11 populations containing three putative varieties of Abies lasiocarpa: (1) var. lasiocarpa, (2) var. bifolia, and (3) var. arizonica. We tested primers from related taxa, and 13 of the best primer pairs were used for assays. Within populations, both heterozygosity and allelic richness were approximately 10% lower in var. lasiocarpa. The STRUCTURE procedure struggled to assign populations to groups correctly; at K = 3, individuals were assigned to their putative varieties with approximately 70% accuracy. Regardless, both lasiocarpa and bifolia were correctly assigned more than expected by chance, indicating that these taxa are distinct. A dendrogram of genetic distances showed var. arizonica to exhibit higher evolutionary distance from the other two varieties and serves as an outgroup. The dendrogram also showed a nesting of var. bifolia clades within var. lasiocarpa, indicating a complex relationship between var. lasiocarpa and var. bifolia. Comparisons among the STRUCTURE population assignments for K = 2, K = 3, and K = 4 identified populations with cryptic admixture and indicate a “ lasiocarpa–bifolia” subspecies complex that warrants further study. Article in Journal/Newspaper Tundra Canadian Science Publishing (via Crossref) Parkland ENVELOPE(-120.570,-120.570,55.917,55.917) Canadian Journal of Forest Research |
institution |
Open Polar |
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Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Ecology Forestry Global and Planetary Change |
spellingShingle |
Ecology Forestry Global and Planetary Change Ritland, Kermit Miscampbell, Allyson Cartwright, Charlie Bohlmann, Nikhil Ritland, Carol Subalpine fir microsatellite variation reveals the complex relationship between var. lasiocarpa and var. bifolia |
topic_facet |
Ecology Forestry Global and Planetary Change |
description |
Subalpine fir, Abies lasiocarpa, occurs throughout western North America, often in forest–tundra parkland. To resolve the presence of varieties in this species, we surveyed microsatellite genetic markers in 11 populations containing three putative varieties of Abies lasiocarpa: (1) var. lasiocarpa, (2) var. bifolia, and (3) var. arizonica. We tested primers from related taxa, and 13 of the best primer pairs were used for assays. Within populations, both heterozygosity and allelic richness were approximately 10% lower in var. lasiocarpa. The STRUCTURE procedure struggled to assign populations to groups correctly; at K = 3, individuals were assigned to their putative varieties with approximately 70% accuracy. Regardless, both lasiocarpa and bifolia were correctly assigned more than expected by chance, indicating that these taxa are distinct. A dendrogram of genetic distances showed var. arizonica to exhibit higher evolutionary distance from the other two varieties and serves as an outgroup. The dendrogram also showed a nesting of var. bifolia clades within var. lasiocarpa, indicating a complex relationship between var. lasiocarpa and var. bifolia. Comparisons among the STRUCTURE population assignments for K = 2, K = 3, and K = 4 identified populations with cryptic admixture and indicate a “ lasiocarpa–bifolia” subspecies complex that warrants further study. |
author2 |
Natural Resources and Engineering Research Council of Canada Ministry of Forests, Lands, Natural Resource Operations and Rural Development |
format |
Article in Journal/Newspaper |
author |
Ritland, Kermit Miscampbell, Allyson Cartwright, Charlie Bohlmann, Nikhil Ritland, Carol |
author_facet |
Ritland, Kermit Miscampbell, Allyson Cartwright, Charlie Bohlmann, Nikhil Ritland, Carol |
author_sort |
Ritland, Kermit |
title |
Subalpine fir microsatellite variation reveals the complex relationship between var. lasiocarpa and var. bifolia |
title_short |
Subalpine fir microsatellite variation reveals the complex relationship between var. lasiocarpa and var. bifolia |
title_full |
Subalpine fir microsatellite variation reveals the complex relationship between var. lasiocarpa and var. bifolia |
title_fullStr |
Subalpine fir microsatellite variation reveals the complex relationship between var. lasiocarpa and var. bifolia |
title_full_unstemmed |
Subalpine fir microsatellite variation reveals the complex relationship between var. lasiocarpa and var. bifolia |
title_sort |
subalpine fir microsatellite variation reveals the complex relationship between var. lasiocarpa and var. bifolia |
publisher |
Canadian Science Publishing |
publishDate |
2022 |
url |
http://dx.doi.org/10.1139/cjfr-2021-0303 https://cdnsciencepub.com/doi/full-xml/10.1139/cjfr-2021-0303 https://cdnsciencepub.com/doi/pdf/10.1139/cjfr-2021-0303 |
long_lat |
ENVELOPE(-120.570,-120.570,55.917,55.917) |
geographic |
Parkland |
geographic_facet |
Parkland |
genre |
Tundra |
genre_facet |
Tundra |
op_source |
Canadian Journal of Forest Research volume 52, issue 6, page 901-909 ISSN 0045-5067 1208-6037 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/cjfr-2021-0303 |
container_title |
Canadian Journal of Forest Research |
_version_ |
1785576404203601920 |