At the forefront: evidence of the applicability of using environmental DNA to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations
Environmental DNA (eDNA) sampling has proven to be a valuable tool for detecting species in aquatic ecosystems. Within this rapidly evolving field, a promising application is the ability to obtain quantitative estimates of relative species abundance based on eDNA concentration rather than traditiona...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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Language: | English |
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Canadian Science Publishing
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Online Access: | http://dx.doi.org/10.1139/cjfas-2017-0114 http://www.nrcresearchpress.com/doi/full-xml/10.1139/cjfas-2017-0114 http://www.nrcresearchpress.com/doi/pdf/10.1139/cjfas-2017-0114 |
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crcansciencepubl:10.1139/cjfas-2017-0114 2024-04-07T07:50:25+00:00 At the forefront: evidence of the applicability of using environmental DNA to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations Klobucar, Stephen L. Rodgers, Torrey W. Budy, Phaedra 2017 http://dx.doi.org/10.1139/cjfas-2017-0114 http://www.nrcresearchpress.com/doi/full-xml/10.1139/cjfas-2017-0114 http://www.nrcresearchpress.com/doi/pdf/10.1139/cjfas-2017-0114 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 74, issue 12, page 2030-2034 ISSN 0706-652X 1205-7533 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 2017 crcansciencepubl https://doi.org/10.1139/cjfas-2017-0114 2024-03-08T00:37:47Z Environmental DNA (eDNA) sampling has proven to be a valuable tool for detecting species in aquatic ecosystems. Within this rapidly evolving field, a promising application is the ability to obtain quantitative estimates of relative species abundance based on eDNA concentration rather than traditionally labor-intensive methods. We investigated the relationship between eDNA concentration and Arctic char (Salvelinus alpinus) abundance in five well-studied natural lakes; additionally, we examined the effects of different temporal (e.g., season) and spatial (e.g., depth) scales on eDNA concentration. Concentrations of eDNA were linearly correlated with char population estimates ([Formula: see text] = 0.78) and exponentially correlated with char densities ([Formula: see text] = 0.96 by area; 0.82 by volume). Across lakes, eDNA concentrations were greater and more homogeneous in the water column during mixis; however, when stratified, eDNA concentrations were greater in the hypolimnion. Overall, our findings demonstrate that eDNA techniques can produce effective estimates of relative fish abundance in natural lakes. These findings can guide future studies to improve and expand eDNA methods while informing research and management using rapid and minimally invasive sampling. Article in Journal/Newspaper Arctic Salvelinus alpinus Canadian Science Publishing Arctic Canadian Journal of Fisheries and Aquatic Sciences 74 12 2030 2034 |
institution |
Open Polar |
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Canadian Science Publishing |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics Klobucar, Stephen L. Rodgers, Torrey W. Budy, Phaedra At the forefront: evidence of the applicability of using environmental DNA to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
Environmental DNA (eDNA) sampling has proven to be a valuable tool for detecting species in aquatic ecosystems. Within this rapidly evolving field, a promising application is the ability to obtain quantitative estimates of relative species abundance based on eDNA concentration rather than traditionally labor-intensive methods. We investigated the relationship between eDNA concentration and Arctic char (Salvelinus alpinus) abundance in five well-studied natural lakes; additionally, we examined the effects of different temporal (e.g., season) and spatial (e.g., depth) scales on eDNA concentration. Concentrations of eDNA were linearly correlated with char population estimates ([Formula: see text] = 0.78) and exponentially correlated with char densities ([Formula: see text] = 0.96 by area; 0.82 by volume). Across lakes, eDNA concentrations were greater and more homogeneous in the water column during mixis; however, when stratified, eDNA concentrations were greater in the hypolimnion. Overall, our findings demonstrate that eDNA techniques can produce effective estimates of relative fish abundance in natural lakes. These findings can guide future studies to improve and expand eDNA methods while informing research and management using rapid and minimally invasive sampling. |
format |
Article in Journal/Newspaper |
author |
Klobucar, Stephen L. Rodgers, Torrey W. Budy, Phaedra |
author_facet |
Klobucar, Stephen L. Rodgers, Torrey W. Budy, Phaedra |
author_sort |
Klobucar, Stephen L. |
title |
At the forefront: evidence of the applicability of using environmental DNA to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations |
title_short |
At the forefront: evidence of the applicability of using environmental DNA to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations |
title_full |
At the forefront: evidence of the applicability of using environmental DNA to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations |
title_fullStr |
At the forefront: evidence of the applicability of using environmental DNA to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations |
title_full_unstemmed |
At the forefront: evidence of the applicability of using environmental DNA to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations |
title_sort |
at the forefront: evidence of the applicability of using environmental dna to quantify the abundance of fish populations in natural lentic waters with additional sampling considerations |
publisher |
Canadian Science Publishing |
publishDate |
2017 |
url |
http://dx.doi.org/10.1139/cjfas-2017-0114 http://www.nrcresearchpress.com/doi/full-xml/10.1139/cjfas-2017-0114 http://www.nrcresearchpress.com/doi/pdf/10.1139/cjfas-2017-0114 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Salvelinus alpinus |
genre_facet |
Arctic Salvelinus alpinus |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 74, issue 12, page 2030-2034 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/cjfas-2017-0114 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
container_volume |
74 |
container_issue |
12 |
container_start_page |
2030 |
op_container_end_page |
2034 |
_version_ |
1795665118259838976 |