Development of a sandwich hybridization assay for the identification and quantification of red drum ( Sciaenops ocellatus ) eggs: a novel tool for fishery research and management
Egg identification and quantification are crucial to understanding the spawning and recruitment dynamics of economically important fish species. This study describes the development of a novel molecular method for finfish egg identification that eliminates the need for time-consuming microscopy. San...
Published in: | Canadian Journal of Fisheries and Aquatic Sciences |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Canadian Science Publishing
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Online Access: | http://dx.doi.org/10.1139/cjfas-2014-0526 http://www.nrcresearchpress.com/doi/full-xml/10.1139/cjfas-2014-0526 http://www.nrcresearchpress.com/doi/pdf/10.1139/cjfas-2014-0526 |
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crcansciencepubl:10.1139/cjfas-2014-0526 2023-12-17T10:49:14+01:00 Development of a sandwich hybridization assay for the identification and quantification of red drum ( Sciaenops ocellatus ) eggs: a novel tool for fishery research and management Mortensen, Rebecca A. Arnott, Stephen A. Jones, William J. Greenfield, Dianne I. MacLatchy, Deborah 2015 http://dx.doi.org/10.1139/cjfas-2014-0526 http://www.nrcresearchpress.com/doi/full-xml/10.1139/cjfas-2014-0526 http://www.nrcresearchpress.com/doi/pdf/10.1139/cjfas-2014-0526 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Fisheries and Aquatic Sciences volume 72, issue 6, page 915-925 ISSN 0706-652X 1205-7533 Aquatic Science Ecology, Evolution, Behavior and Systematics journal-article 2015 crcansciencepubl https://doi.org/10.1139/cjfas-2014-0526 2023-11-19T13:38:54Z Egg identification and quantification are crucial to understanding the spawning and recruitment dynamics of economically important fish species. This study describes the development of a novel molecular method for finfish egg identification that eliminates the need for time-consuming microscopy. Sandwich hybridization assay (SHA) uses two ribosomal RNA (rRNA)-targeted oligonucleotides to directly detect unpurified and unamplified rRNA. Probes were designed to complement the internal transcribed spacer (ITS) region of the red drum (Sciaenops ocellatus), an important recreational game fish for which spawning and reproductive information is sparse. Sample homogenization procedures were modified to disrupt egg chorion, and the resultant assay detected S. ocellatus eggs and tissues without cross-reactivity. Standard curves were linear (y 450 = 0.001x + 0.054; R 2 = 0.999), showing potential for quantitative uses, and the lower limit of detection was 5 eggs·mL −1 homogenate. Ontogenetic stage had a significant effect (ANOVA, p < 0.05) on optical density. The assay successfully detected S.ocellatus eggs in field samples from Charleston Harbor, South Carolina, and could be incorporated into current management practices or adapted to other species. Article in Journal/Newspaper Red drum Sciaenops ocellatus Canadian Science Publishing (via Crossref) Canadian Journal of Fisheries and Aquatic Sciences 72 6 915 925 |
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Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
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crcansciencepubl |
language |
English |
topic |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Aquatic Science Ecology, Evolution, Behavior and Systematics Mortensen, Rebecca A. Arnott, Stephen A. Jones, William J. Greenfield, Dianne I. Development of a sandwich hybridization assay for the identification and quantification of red drum ( Sciaenops ocellatus ) eggs: a novel tool for fishery research and management |
topic_facet |
Aquatic Science Ecology, Evolution, Behavior and Systematics |
description |
Egg identification and quantification are crucial to understanding the spawning and recruitment dynamics of economically important fish species. This study describes the development of a novel molecular method for finfish egg identification that eliminates the need for time-consuming microscopy. Sandwich hybridization assay (SHA) uses two ribosomal RNA (rRNA)-targeted oligonucleotides to directly detect unpurified and unamplified rRNA. Probes were designed to complement the internal transcribed spacer (ITS) region of the red drum (Sciaenops ocellatus), an important recreational game fish for which spawning and reproductive information is sparse. Sample homogenization procedures were modified to disrupt egg chorion, and the resultant assay detected S. ocellatus eggs and tissues without cross-reactivity. Standard curves were linear (y 450 = 0.001x + 0.054; R 2 = 0.999), showing potential for quantitative uses, and the lower limit of detection was 5 eggs·mL −1 homogenate. Ontogenetic stage had a significant effect (ANOVA, p < 0.05) on optical density. The assay successfully detected S.ocellatus eggs in field samples from Charleston Harbor, South Carolina, and could be incorporated into current management practices or adapted to other species. |
author2 |
MacLatchy, Deborah |
format |
Article in Journal/Newspaper |
author |
Mortensen, Rebecca A. Arnott, Stephen A. Jones, William J. Greenfield, Dianne I. |
author_facet |
Mortensen, Rebecca A. Arnott, Stephen A. Jones, William J. Greenfield, Dianne I. |
author_sort |
Mortensen, Rebecca A. |
title |
Development of a sandwich hybridization assay for the identification and quantification of red drum ( Sciaenops ocellatus ) eggs: a novel tool for fishery research and management |
title_short |
Development of a sandwich hybridization assay for the identification and quantification of red drum ( Sciaenops ocellatus ) eggs: a novel tool for fishery research and management |
title_full |
Development of a sandwich hybridization assay for the identification and quantification of red drum ( Sciaenops ocellatus ) eggs: a novel tool for fishery research and management |
title_fullStr |
Development of a sandwich hybridization assay for the identification and quantification of red drum ( Sciaenops ocellatus ) eggs: a novel tool for fishery research and management |
title_full_unstemmed |
Development of a sandwich hybridization assay for the identification and quantification of red drum ( Sciaenops ocellatus ) eggs: a novel tool for fishery research and management |
title_sort |
development of a sandwich hybridization assay for the identification and quantification of red drum ( sciaenops ocellatus ) eggs: a novel tool for fishery research and management |
publisher |
Canadian Science Publishing |
publishDate |
2015 |
url |
http://dx.doi.org/10.1139/cjfas-2014-0526 http://www.nrcresearchpress.com/doi/full-xml/10.1139/cjfas-2014-0526 http://www.nrcresearchpress.com/doi/pdf/10.1139/cjfas-2014-0526 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_source |
Canadian Journal of Fisheries and Aquatic Sciences volume 72, issue 6, page 915-925 ISSN 0706-652X 1205-7533 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/cjfas-2014-0526 |
container_title |
Canadian Journal of Fisheries and Aquatic Sciences |
container_volume |
72 |
container_issue |
6 |
container_start_page |
915 |
op_container_end_page |
925 |
_version_ |
1785573638315966464 |