In vitro plantlet regeneration from hypocotyl explants of Stellaria longipes (Caryophyllaceae)

• Published in: Canadian Journal of Botany
• Main Authors: Miranda, Jacintha, Konschuh, Michele N, Yeung, E C, Chinnappa, C C
• Format: Article in Journal/Newspaper
• Language: French
• Published: Canadian Science Publishing 1999
• Subjects: Plant Science; Goldie; Stellaria longipes
• Online Access: http://dx.doi.org/10.1139/b99-024; http://www.nrcresearchpress.com/doi/pdf/10.1139/b99-024

An in vitro regeneration protocol for Stellaria longipes Goldie was developed using young hypocotyl explants. Optimal regeneration was obtained using Murashige and Skoog (MS) basal medium supplemented with 0.5 µM N 6 -benzyladenine and 1 µM indole-3-butyric acid. Three different patterns of shoot regeneration were observed: (i) "direct shoot" formation within 3-5 days of inoculation, (ii) nodular structures appeared followed by shoot formation, and (iii) callus formation followed by the appearance of shoots. Histological observation revealed that cells within the central vascular cylinder of the hypocotyl were responsible for shoot organogenesis. Shoot production was not synchronous or uniform among explants. A more synchronous shoot production was obtained by excising the direct shoots or by wounding the nodular structures. Excision and wounding increased the regeneration capability of the explants. Regenerated shoots were readily rooted in MS medium lacking growth regulators and were successfully transferred to greenhouse conditions. These showed morphology consistence with greenhouse-grown plants.Key words: hypocotyl, organogenesis, regeneration, Stellaria longipes.