Ecotypic differences in rhythmicity of ethylene production in Stellaria longipes : the possible roles of ACC, MACC, and ACC oxidase
The alpine tundra ecotype of Stellaria longipes is characterized by a dwarf phenotype, whereas the prairie ecotype can be semidwarf or highly elongated depending on its environment. Related to their ability to elongate, these ecotypes also show divergent abilities to produce and respond to ethylene....
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1997
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crcansciencepubl:10.1139/b97-114 2023-12-17T10:50:42+01:00 Ecotypic differences in rhythmicity of ethylene production in Stellaria longipes : the possible roles of ACC, MACC, and ACC oxidase Emery, R. J. N. Kathiresan, A. Reid, D. M. Chinnappa, C. C. 1997 http://dx.doi.org/10.1139/b97-114 http://www.nrcresearchpress.com/doi/pdf/10.1139/b97-114 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Botany volume 75, issue 7, page 1027-1033 ISSN 0008-4026 Plant Science journal-article 1997 crcansciencepubl https://doi.org/10.1139/b97-114 2023-11-19T13:38:55Z The alpine tundra ecotype of Stellaria longipes is characterized by a dwarf phenotype, whereas the prairie ecotype can be semidwarf or highly elongated depending on its environment. Related to their ability to elongate, these ecotypes also show divergent abilities to produce and respond to ethylene. The prairie ecotype produces a strong daily rhythm of ethylene, which is maintained even following stress events such as wind. The alpine ecotype exhibits a much less pronounced rhythm but greatly increases ethylene production in response to stress. We investigated what differences in ethylene synthesis might be responsible for the ability of the prairie ecotype to produce a large and regular daily rhythm of ethylene production, which in the alpine ecotype is weaker or sometimes absent. Levels of the immediate precursor to ethylene, 1-aminocyclopropane-1-carboxylate (ACC), and its major conjugate, malonyl ACC (MACC) showed no rhythm across the course of a day. Moreover ACC levels remained stable during an entire growth cycle (21 days) in the prairie ecotype, even though ethylene is known to increase especially during periods of rapid elongation. By contrast, assays of ACC oxidase performed in vivo and in vitro showed rhythms of activity similar to those of ethylene production observed in the prairie ecotype. However, the levels of ethylene produced in the ACC oxidase assays were considerably higher than levels of ethylene normally produced by unstressed plants, and the rhythm of ACC oxidase activity was observed in both ecotypes, despite the fact that alpine Stellaria longipes exhibits a less pronounced ethylene rhythm. Thus, we concluded that although ACC oxidase activity may partially account for rhythmic production of ethylene in prairie ecotypes, other controlling factors such as spatial separation of ACC from ACC oxidase should be investigated. Key words: ACC oxidase, ecotypes, ethylene, phenotypic plasticity, rhythmicity, Stellaria longipes. Article in Journal/Newspaper Stellaria longipes Tundra Canadian Science Publishing (via Crossref) Canadian Journal of Botany 75 7 1027 1033 |
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Canadian Science Publishing (via Crossref) |
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crcansciencepubl |
language |
English |
topic |
Plant Science |
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Plant Science Emery, R. J. N. Kathiresan, A. Reid, D. M. Chinnappa, C. C. Ecotypic differences in rhythmicity of ethylene production in Stellaria longipes : the possible roles of ACC, MACC, and ACC oxidase |
topic_facet |
Plant Science |
description |
The alpine tundra ecotype of Stellaria longipes is characterized by a dwarf phenotype, whereas the prairie ecotype can be semidwarf or highly elongated depending on its environment. Related to their ability to elongate, these ecotypes also show divergent abilities to produce and respond to ethylene. The prairie ecotype produces a strong daily rhythm of ethylene, which is maintained even following stress events such as wind. The alpine ecotype exhibits a much less pronounced rhythm but greatly increases ethylene production in response to stress. We investigated what differences in ethylene synthesis might be responsible for the ability of the prairie ecotype to produce a large and regular daily rhythm of ethylene production, which in the alpine ecotype is weaker or sometimes absent. Levels of the immediate precursor to ethylene, 1-aminocyclopropane-1-carboxylate (ACC), and its major conjugate, malonyl ACC (MACC) showed no rhythm across the course of a day. Moreover ACC levels remained stable during an entire growth cycle (21 days) in the prairie ecotype, even though ethylene is known to increase especially during periods of rapid elongation. By contrast, assays of ACC oxidase performed in vivo and in vitro showed rhythms of activity similar to those of ethylene production observed in the prairie ecotype. However, the levels of ethylene produced in the ACC oxidase assays were considerably higher than levels of ethylene normally produced by unstressed plants, and the rhythm of ACC oxidase activity was observed in both ecotypes, despite the fact that alpine Stellaria longipes exhibits a less pronounced ethylene rhythm. Thus, we concluded that although ACC oxidase activity may partially account for rhythmic production of ethylene in prairie ecotypes, other controlling factors such as spatial separation of ACC from ACC oxidase should be investigated. Key words: ACC oxidase, ecotypes, ethylene, phenotypic plasticity, rhythmicity, Stellaria longipes. |
format |
Article in Journal/Newspaper |
author |
Emery, R. J. N. Kathiresan, A. Reid, D. M. Chinnappa, C. C. |
author_facet |
Emery, R. J. N. Kathiresan, A. Reid, D. M. Chinnappa, C. C. |
author_sort |
Emery, R. J. N. |
title |
Ecotypic differences in rhythmicity of ethylene production in Stellaria longipes : the possible roles of ACC, MACC, and ACC oxidase |
title_short |
Ecotypic differences in rhythmicity of ethylene production in Stellaria longipes : the possible roles of ACC, MACC, and ACC oxidase |
title_full |
Ecotypic differences in rhythmicity of ethylene production in Stellaria longipes : the possible roles of ACC, MACC, and ACC oxidase |
title_fullStr |
Ecotypic differences in rhythmicity of ethylene production in Stellaria longipes : the possible roles of ACC, MACC, and ACC oxidase |
title_full_unstemmed |
Ecotypic differences in rhythmicity of ethylene production in Stellaria longipes : the possible roles of ACC, MACC, and ACC oxidase |
title_sort |
ecotypic differences in rhythmicity of ethylene production in stellaria longipes : the possible roles of acc, macc, and acc oxidase |
publisher |
Canadian Science Publishing |
publishDate |
1997 |
url |
http://dx.doi.org/10.1139/b97-114 http://www.nrcresearchpress.com/doi/pdf/10.1139/b97-114 |
genre |
Stellaria longipes Tundra |
genre_facet |
Stellaria longipes Tundra |
op_source |
Canadian Journal of Botany volume 75, issue 7, page 1027-1033 ISSN 0008-4026 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/b97-114 |
container_title |
Canadian Journal of Botany |
container_volume |
75 |
container_issue |
7 |
container_start_page |
1027 |
op_container_end_page |
1033 |
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1785575745804828672 |