Two lipases from Candida antarctica: cloning and expression in Aspergillus oryzae
The basidiomycetous yeast Candida antarctica expresses two lipases that possess widely different properties. The genes LIPA and LIPB encoding both lipases were cloned and sequenced. Both lipases were secreted efficiently from Aspergillus oryzae transformed with lipase expression plasmids. N-Glycosyl...
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Canadian Science Publishing
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Online Access: | http://dx.doi.org/10.1139/b95-333 http://www.nrcresearchpress.com/doi/pdf/10.1139/b95-333 |
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crcansciencepubl:10.1139/b95-333 2024-06-23T07:47:34+00:00 Two lipases from Candida antarctica: cloning and expression in Aspergillus oryzae Høegh, Inge Patkar, Shamkant Halkier, Torben Hansen, Mogens T. 1995 http://dx.doi.org/10.1139/b95-333 http://www.nrcresearchpress.com/doi/pdf/10.1139/b95-333 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Botany volume 73, issue S1, page 869-875 ISSN 0008-4026 journal-article 1995 crcansciencepubl https://doi.org/10.1139/b95-333 2024-05-24T13:05:52Z The basidiomycetous yeast Candida antarctica expresses two lipases that possess widely different properties. The genes LIPA and LIPB encoding both lipases were cloned and sequenced. Both lipases were secreted efficiently from Aspergillus oryzae transformed with lipase expression plasmids. N-Glycosylation was slightly more extensive in the heterologously expressed enzymes than in those purified from C. antarctica, but the enzymatic characteristics were retained. Both enzymes are encoded as preproenzymes. Proteolytic processing of the primary translation product was efficient in A. oryzae and resulted in the same N-terminals as in C. antarctica. Modifications or deletions of the propeptide of lipase component B did not prevent efficient secretion of active lipase from A. oryzae. Alternative proteolytic processing of the modified propeptides was detected. Key words: Lipase, Candida, cloning, Aspergillus, expression, propeptide. Article in Journal/Newspaper Antarc* Antarctica Canadian Science Publishing Canadian Journal of Botany 73 S1 869 875 |
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Open Polar |
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Canadian Science Publishing |
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crcansciencepubl |
language |
English |
description |
The basidiomycetous yeast Candida antarctica expresses two lipases that possess widely different properties. The genes LIPA and LIPB encoding both lipases were cloned and sequenced. Both lipases were secreted efficiently from Aspergillus oryzae transformed with lipase expression plasmids. N-Glycosylation was slightly more extensive in the heterologously expressed enzymes than in those purified from C. antarctica, but the enzymatic characteristics were retained. Both enzymes are encoded as preproenzymes. Proteolytic processing of the primary translation product was efficient in A. oryzae and resulted in the same N-terminals as in C. antarctica. Modifications or deletions of the propeptide of lipase component B did not prevent efficient secretion of active lipase from A. oryzae. Alternative proteolytic processing of the modified propeptides was detected. Key words: Lipase, Candida, cloning, Aspergillus, expression, propeptide. |
format |
Article in Journal/Newspaper |
author |
Høegh, Inge Patkar, Shamkant Halkier, Torben Hansen, Mogens T. |
spellingShingle |
Høegh, Inge Patkar, Shamkant Halkier, Torben Hansen, Mogens T. Two lipases from Candida antarctica: cloning and expression in Aspergillus oryzae |
author_facet |
Høegh, Inge Patkar, Shamkant Halkier, Torben Hansen, Mogens T. |
author_sort |
Høegh, Inge |
title |
Two lipases from Candida antarctica: cloning and expression in Aspergillus oryzae |
title_short |
Two lipases from Candida antarctica: cloning and expression in Aspergillus oryzae |
title_full |
Two lipases from Candida antarctica: cloning and expression in Aspergillus oryzae |
title_fullStr |
Two lipases from Candida antarctica: cloning and expression in Aspergillus oryzae |
title_full_unstemmed |
Two lipases from Candida antarctica: cloning and expression in Aspergillus oryzae |
title_sort |
two lipases from candida antarctica: cloning and expression in aspergillus oryzae |
publisher |
Canadian Science Publishing |
publishDate |
1995 |
url |
http://dx.doi.org/10.1139/b95-333 http://www.nrcresearchpress.com/doi/pdf/10.1139/b95-333 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Canadian Journal of Botany volume 73, issue S1, page 869-875 ISSN 0008-4026 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/b95-333 |
container_title |
Canadian Journal of Botany |
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73 |
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S1 |
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869 |
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875 |
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1802651693392855040 |