The characterization of the heat-shock response in the arctic plant Saxifraga cernua
A rapid increase in the incubation temperature of whole leaves of the arctic plant Saxifraga cernua, grown at 10 or 20 °C, resulted in the noncoordinate expression of 21 novel and (or) enhanced heat-shock polypeptides. Unlike other organisms, the expression of most of the normal polypeptides was not...
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Canadian Science Publishing
1990
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crcansciencepubl:10.1139/b90-009 2023-12-17T10:24:59+01:00 The characterization of the heat-shock response in the arctic plant Saxifraga cernua Mason-Apps, Susan Cummins, W. R. Filion, W. Gary 1990 http://dx.doi.org/10.1139/b90-009 http://www.nrcresearchpress.com/doi/pdf/10.1139/b90-009 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Botany volume 68, issue 1, page 56-62 ISSN 0008-4026 Plant Science journal-article 1990 crcansciencepubl https://doi.org/10.1139/b90-009 2023-11-19T13:38:57Z A rapid increase in the incubation temperature of whole leaves of the arctic plant Saxifraga cernua, grown at 10 or 20 °C, resulted in the noncoordinate expression of 21 novel and (or) enhanced heat-shock polypeptides. Unlike other organisms, the expression of most of the normal polypeptides was not reduced. Incorporation of radioactivity during hyperthermic shifts was similar in both groups of plants at all temperatures investigated, with peak incorporation recorded at 33 °C. The temperature at which the expression of the heat-shock polypeptides was at a maximum level was also 33 °C. Protein synthesis was reduced after a heat shock at 35 °C and completely inhibited at 38 °C. These data suggest that the preshock growth temperature had no effect on the temperature of maximum expression of the heat-shock polypeptides nor the level of protein synthesis during heat shock. Recovery at 10 °C from a 33 °C heat shock was complete within 7–9 h. Extended time periods (up to 24 h) at the shock temperature of 33 °C demonstrated that the heat-shock response in this arctic plant was multiphasic, consisting of an early group and five sets of late heat-shock polypeptides Article in Journal/Newspaper Arctic Saxifraga cernua Canadian Science Publishing (via Crossref) Arctic Canadian Journal of Botany 68 1 56 62 |
institution |
Open Polar |
collection |
Canadian Science Publishing (via Crossref) |
op_collection_id |
crcansciencepubl |
language |
English |
topic |
Plant Science |
spellingShingle |
Plant Science Mason-Apps, Susan Cummins, W. R. Filion, W. Gary The characterization of the heat-shock response in the arctic plant Saxifraga cernua |
topic_facet |
Plant Science |
description |
A rapid increase in the incubation temperature of whole leaves of the arctic plant Saxifraga cernua, grown at 10 or 20 °C, resulted in the noncoordinate expression of 21 novel and (or) enhanced heat-shock polypeptides. Unlike other organisms, the expression of most of the normal polypeptides was not reduced. Incorporation of radioactivity during hyperthermic shifts was similar in both groups of plants at all temperatures investigated, with peak incorporation recorded at 33 °C. The temperature at which the expression of the heat-shock polypeptides was at a maximum level was also 33 °C. Protein synthesis was reduced after a heat shock at 35 °C and completely inhibited at 38 °C. These data suggest that the preshock growth temperature had no effect on the temperature of maximum expression of the heat-shock polypeptides nor the level of protein synthesis during heat shock. Recovery at 10 °C from a 33 °C heat shock was complete within 7–9 h. Extended time periods (up to 24 h) at the shock temperature of 33 °C demonstrated that the heat-shock response in this arctic plant was multiphasic, consisting of an early group and five sets of late heat-shock polypeptides |
format |
Article in Journal/Newspaper |
author |
Mason-Apps, Susan Cummins, W. R. Filion, W. Gary |
author_facet |
Mason-Apps, Susan Cummins, W. R. Filion, W. Gary |
author_sort |
Mason-Apps, Susan |
title |
The characterization of the heat-shock response in the arctic plant Saxifraga cernua |
title_short |
The characterization of the heat-shock response in the arctic plant Saxifraga cernua |
title_full |
The characterization of the heat-shock response in the arctic plant Saxifraga cernua |
title_fullStr |
The characterization of the heat-shock response in the arctic plant Saxifraga cernua |
title_full_unstemmed |
The characterization of the heat-shock response in the arctic plant Saxifraga cernua |
title_sort |
characterization of the heat-shock response in the arctic plant saxifraga cernua |
publisher |
Canadian Science Publishing |
publishDate |
1990 |
url |
http://dx.doi.org/10.1139/b90-009 http://www.nrcresearchpress.com/doi/pdf/10.1139/b90-009 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Saxifraga cernua |
genre_facet |
Arctic Saxifraga cernua |
op_source |
Canadian Journal of Botany volume 68, issue 1, page 56-62 ISSN 0008-4026 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/b90-009 |
container_title |
Canadian Journal of Botany |
container_volume |
68 |
container_issue |
1 |
container_start_page |
56 |
op_container_end_page |
62 |
_version_ |
1785572021370880000 |