The effect of thallus age and drying procedure on extractable lichen substances
Cladonia rangiferina (L.)Wigg., Cladonia mitis Sandst., and Umbilicaria muhlenbergii (Ach.) Tuck, were extracted with acetone while damp and after three different drying procedures. The extracts were chromatographed and scanned spectrophotometrically. Some of the C. rangiferina compounds were not ex...
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Canadian Science Publishing
1978
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crcansciencepubl:10.1139/b78-175 2024-09-15T18:02:09+00:00 The effect of thallus age and drying procedure on extractable lichen substances Mirando, Martha Fahselt, Dianne 1978 http://dx.doi.org/10.1139/b78-175 http://www.nrcresearchpress.com/doi/pdf/10.1139/b78-175 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Botany volume 56, issue 13, page 1499-1504 ISSN 0008-4026 journal-article 1978 crcansciencepubl https://doi.org/10.1139/b78-175 2024-08-08T04:13:41Z Cladonia rangiferina (L.)Wigg., Cladonia mitis Sandst., and Umbilicaria muhlenbergii (Ach.) Tuck, were extracted with acetone while damp and after three different drying procedures. The extracts were chromatographed and scanned spectrophotometrically. Some of the C. rangiferina compounds were not extractable from damp material. All of the lichen substances studied here decreased significantly in thalli which were dried in an oven at 80 °C, some to the point of imperceptibility. There was no qualitative difference between the complement of compounds found in lichen material dried at 22 °C and at 40 °C. We recommend that drying be carried out at a temperature not exceeding 40 °C. Chemical variability among thallus regions of different ages was studied in Cladonia rangiferina, C. mitis, Umbilicaria muhlenbergii, and Lasallia papulosa (Ach.) Llano. Fumarprotocetraric acid, atranorin, and usnic acid were present in significantly higher amounts in younger tissue than in older. Protocetraric acid in C. rangiferina was detectable only in the older portions and would have been overlooked if young branches had been analyzed exclusively. In neither of the two umbilicate lichens was there any significant difference in the concentration of gyrophoric acid between younger and older thallus parts. Chemical variability among different-aged thallus parts was thus greater in the fruticose species than in the umbilicates examined here. Article in Journal/Newspaper Cladonia rangiferina Canadian Science Publishing Canadian Journal of Botany 56 13 1499 1504 |
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Canadian Science Publishing |
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English |
description |
Cladonia rangiferina (L.)Wigg., Cladonia mitis Sandst., and Umbilicaria muhlenbergii (Ach.) Tuck, were extracted with acetone while damp and after three different drying procedures. The extracts were chromatographed and scanned spectrophotometrically. Some of the C. rangiferina compounds were not extractable from damp material. All of the lichen substances studied here decreased significantly in thalli which were dried in an oven at 80 °C, some to the point of imperceptibility. There was no qualitative difference between the complement of compounds found in lichen material dried at 22 °C and at 40 °C. We recommend that drying be carried out at a temperature not exceeding 40 °C. Chemical variability among thallus regions of different ages was studied in Cladonia rangiferina, C. mitis, Umbilicaria muhlenbergii, and Lasallia papulosa (Ach.) Llano. Fumarprotocetraric acid, atranorin, and usnic acid were present in significantly higher amounts in younger tissue than in older. Protocetraric acid in C. rangiferina was detectable only in the older portions and would have been overlooked if young branches had been analyzed exclusively. In neither of the two umbilicate lichens was there any significant difference in the concentration of gyrophoric acid between younger and older thallus parts. Chemical variability among different-aged thallus parts was thus greater in the fruticose species than in the umbilicates examined here. |
format |
Article in Journal/Newspaper |
author |
Mirando, Martha Fahselt, Dianne |
spellingShingle |
Mirando, Martha Fahselt, Dianne The effect of thallus age and drying procedure on extractable lichen substances |
author_facet |
Mirando, Martha Fahselt, Dianne |
author_sort |
Mirando, Martha |
title |
The effect of thallus age and drying procedure on extractable lichen substances |
title_short |
The effect of thallus age and drying procedure on extractable lichen substances |
title_full |
The effect of thallus age and drying procedure on extractable lichen substances |
title_fullStr |
The effect of thallus age and drying procedure on extractable lichen substances |
title_full_unstemmed |
The effect of thallus age and drying procedure on extractable lichen substances |
title_sort |
effect of thallus age and drying procedure on extractable lichen substances |
publisher |
Canadian Science Publishing |
publishDate |
1978 |
url |
http://dx.doi.org/10.1139/b78-175 http://www.nrcresearchpress.com/doi/pdf/10.1139/b78-175 |
genre |
Cladonia rangiferina |
genre_facet |
Cladonia rangiferina |
op_source |
Canadian Journal of Botany volume 56, issue 13, page 1499-1504 ISSN 0008-4026 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/b78-175 |
container_title |
Canadian Journal of Botany |
container_volume |
56 |
container_issue |
13 |
container_start_page |
1499 |
op_container_end_page |
1504 |
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1810439427048079360 |