Cold-adapted rhizobia for nitrogen fixation in temperate regions
Rhizobia from Canadian soils were selected for cold adaptation with the aim of improving productivity of legumes that are subjected to cool temperatures during the growing season. One approach was to use rhizobia associated with legume species indigenous to arctic and subarctic regions: (i) Mesorhiz...
Published in: | Canadian Journal of Botany |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Canadian Science Publishing
2003
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1139/b03-113 http://www.nrcresearchpress.com/doi/pdf/10.1139/b03-113 |
id |
crcansciencepubl:10.1139/b03-113 |
---|---|
record_format |
openpolar |
spelling |
crcansciencepubl:10.1139/b03-113 2024-09-15T18:38:05+00:00 Cold-adapted rhizobia for nitrogen fixation in temperate regions Prévost, Danielle Drouin, Pascal Laberge, Serge Bertrand, Annick Cloutier, Jean Lévesque, Gabriel 2003 http://dx.doi.org/10.1139/b03-113 http://www.nrcresearchpress.com/doi/pdf/10.1139/b03-113 en eng Canadian Science Publishing http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining Canadian Journal of Botany volume 81, issue 12, page 1153-1161 ISSN 0008-4026 journal-article 2003 crcansciencepubl https://doi.org/10.1139/b03-113 2024-08-08T04:13:39Z Rhizobia from Canadian soils were selected for cold adaptation with the aim of improving productivity of legumes that are subjected to cool temperatures during the growing season. One approach was to use rhizobia associated with legume species indigenous to arctic and subarctic regions: (i) Mesorhizobium sp. isolated from Astragalus and Oxytropis spp. and (ii) Rhizobium leguminosarum from Lathryrus spp. The majority of these rhizobia are considered psychrotrophs because they can grow at 0 °C. The advantages of cold adaptation of arctic Mesorhizobium to improve legume symbiosis were demonstrated with the temperate forage legume sainfoin (Onobrychis viciifolia). In laboratory and field studies, arctic rhizobia were more efficient than temperate (commercial) rhizobia in improving growth of sainfoin and were more competitive in forming nodules. Biochemical studies on cold adaptation showed higher synthesis of cold shock proteins in cold-adapted than in nonadapted arctic rhizobia. Since arctic Mesorhizobium cannot nodulate agronomically important legumes, the nodulation genes and the bacterial signals (Nod factors) were characterized as a first step to modifying the host specificity of nodulation. Another valuable approach was to screen for cold adaptation, that is, rhizobia naturally associated with agronomic legumes cultivated in temperate areas. A superior strain of Sinorhizobium meliloti adapted for nodulation of alfalfa at low temperatures was selected and was the most efficient for improving growth of alfalfa in laboratory and field studies. This strain also performed well in improving regrowth of alfalfa after overwintering under cold and anaerobic (ice encasement) stresses, indicating a possible cross-adaptation of selected rhizobia for various abiotic stresses inherent to temperate climates.Key words: cold adaptation, legumes, symbiotic efficiency, cold shock protein, nodulation genes, anaerobiosis. Article in Journal/Newspaper Subarctic Canadian Science Publishing Canadian Journal of Botany 81 12 1153 1161 |
institution |
Open Polar |
collection |
Canadian Science Publishing |
op_collection_id |
crcansciencepubl |
language |
English |
description |
Rhizobia from Canadian soils were selected for cold adaptation with the aim of improving productivity of legumes that are subjected to cool temperatures during the growing season. One approach was to use rhizobia associated with legume species indigenous to arctic and subarctic regions: (i) Mesorhizobium sp. isolated from Astragalus and Oxytropis spp. and (ii) Rhizobium leguminosarum from Lathryrus spp. The majority of these rhizobia are considered psychrotrophs because they can grow at 0 °C. The advantages of cold adaptation of arctic Mesorhizobium to improve legume symbiosis were demonstrated with the temperate forage legume sainfoin (Onobrychis viciifolia). In laboratory and field studies, arctic rhizobia were more efficient than temperate (commercial) rhizobia in improving growth of sainfoin and were more competitive in forming nodules. Biochemical studies on cold adaptation showed higher synthesis of cold shock proteins in cold-adapted than in nonadapted arctic rhizobia. Since arctic Mesorhizobium cannot nodulate agronomically important legumes, the nodulation genes and the bacterial signals (Nod factors) were characterized as a first step to modifying the host specificity of nodulation. Another valuable approach was to screen for cold adaptation, that is, rhizobia naturally associated with agronomic legumes cultivated in temperate areas. A superior strain of Sinorhizobium meliloti adapted for nodulation of alfalfa at low temperatures was selected and was the most efficient for improving growth of alfalfa in laboratory and field studies. This strain also performed well in improving regrowth of alfalfa after overwintering under cold and anaerobic (ice encasement) stresses, indicating a possible cross-adaptation of selected rhizobia for various abiotic stresses inherent to temperate climates.Key words: cold adaptation, legumes, symbiotic efficiency, cold shock protein, nodulation genes, anaerobiosis. |
format |
Article in Journal/Newspaper |
author |
Prévost, Danielle Drouin, Pascal Laberge, Serge Bertrand, Annick Cloutier, Jean Lévesque, Gabriel |
spellingShingle |
Prévost, Danielle Drouin, Pascal Laberge, Serge Bertrand, Annick Cloutier, Jean Lévesque, Gabriel Cold-adapted rhizobia for nitrogen fixation in temperate regions |
author_facet |
Prévost, Danielle Drouin, Pascal Laberge, Serge Bertrand, Annick Cloutier, Jean Lévesque, Gabriel |
author_sort |
Prévost, Danielle |
title |
Cold-adapted rhizobia for nitrogen fixation in temperate regions |
title_short |
Cold-adapted rhizobia for nitrogen fixation in temperate regions |
title_full |
Cold-adapted rhizobia for nitrogen fixation in temperate regions |
title_fullStr |
Cold-adapted rhizobia for nitrogen fixation in temperate regions |
title_full_unstemmed |
Cold-adapted rhizobia for nitrogen fixation in temperate regions |
title_sort |
cold-adapted rhizobia for nitrogen fixation in temperate regions |
publisher |
Canadian Science Publishing |
publishDate |
2003 |
url |
http://dx.doi.org/10.1139/b03-113 http://www.nrcresearchpress.com/doi/pdf/10.1139/b03-113 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
Canadian Journal of Botany volume 81, issue 12, page 1153-1161 ISSN 0008-4026 |
op_rights |
http://www.nrcresearchpress.com/page/about/CorporateTextAndDataMining |
op_doi |
https://doi.org/10.1139/b03-113 |
container_title |
Canadian Journal of Botany |
container_volume |
81 |
container_issue |
12 |
container_start_page |
1153 |
op_container_end_page |
1161 |
_version_ |
1810482411911249920 |