Adrenal responsiveness of reindeer ( Rangifer tarandus tarandus ) to intravenously administered ACTH
Abstract Plasma cortisol concentrations were determined from the blood of eight mature female reindeer ( Rangifer tarandus tarandus ) after an intravenous injection of either saline (control) or 100, 250 or 500 μg of synthetic ACTH. Blood samples were collected at 0, 2, 4, 6, 8, 10, 15, 20, 25, 30,...
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crcambridgeupr:10.1079/asc41870399 2023-05-15T18:04:20+02:00 Adrenal responsiveness of reindeer ( Rangifer tarandus tarandus ) to intravenously administered ACTH Säkkinen, H. Tornberg, J. Goddard, P. J. Eloranta, E. Dahl, E. Ropstad, E. Saarela, S. 2005 http://dx.doi.org/10.1079/asc41870399 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S1357729805000962 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Animal Science volume 81, issue 3, page 399-402 ISSN 1357-7298 1748-748X Animal Science and Zoology journal-article 2005 crcambridgeupr https://doi.org/10.1079/asc41870399 2022-04-07T08:57:36Z Abstract Plasma cortisol concentrations were determined from the blood of eight mature female reindeer ( Rangifer tarandus tarandus ) after an intravenous injection of either saline (control) or 100, 250 or 500 μg of synthetic ACTH. Blood samples were collected at 0, 2, 4, 6, 8, 10, 15, 20, 25, 30, 45, 60, 75, 90, 120, 150, and 180 min after the injections. The aims were to determine the appropriate dose of ACTH for adrenal stimulation tests, to define the dose level of ACTH which elicited a maximal cortisol response and to describe the range of blood cortisol concentrations for reference when evaluating the stress responses of reindeer. The mean plasma cortisol concentrations (s.e.) at the zero sample times (t 0 ) of the control and the ACTH treatments varied between 93·4 (11·8) and 132·5 (18·1) nmol/l. The total plasma cortisol response (area under curve, AUC, nmol/l × min) increased with increasing dose of ACTH ( P < 0·001). The AUC of the control treatment was significantly smaller than of the ACTH treatments ( P < 0·001). The highest dose of ACTH (500 μg) gave a significantly bigger AUC than the lowest dose (100 μg) ( P = 0·008). The maximal plasma cortisol concentrations (CMAX) were achieved within 60 min of the ACTH injections. The ranges of individual CMAX were 59·0 to 136·8 nmol/l for the control treatment, and 110·0 to 252·0, 152·0 to 247·5 and 135·1 to 257·1 nmol/l for 100, 250 and 500 μg ACTH, respectively. The difference in CMAX was significant between the control treatment and the ACTH treatments ( P < 0·001) but not between the different doses of ACTH. The plasma cortisol concentrations at the end of the observation period at t180 were not significantly affected by the ACTH treatment ( P > 0·05). In conclusion, the 100-μg dose of ACTH was sufficient to produce a significant cortisol response compared with the control treatment. Increasing the dose did not increase the maximal response, but tended to elongate the response profile. The blood sampling frequency used in the study was found suitable for detection of the cortisol response in reindeer. Article in Journal/Newspaper Rangifer tarandus Cambridge University Press (via Crossref) Animal Science 81 3 399 402 |
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Cambridge University Press (via Crossref) |
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English |
topic |
Animal Science and Zoology |
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Animal Science and Zoology Säkkinen, H. Tornberg, J. Goddard, P. J. Eloranta, E. Dahl, E. Ropstad, E. Saarela, S. Adrenal responsiveness of reindeer ( Rangifer tarandus tarandus ) to intravenously administered ACTH |
topic_facet |
Animal Science and Zoology |
description |
Abstract Plasma cortisol concentrations were determined from the blood of eight mature female reindeer ( Rangifer tarandus tarandus ) after an intravenous injection of either saline (control) or 100, 250 or 500 μg of synthetic ACTH. Blood samples were collected at 0, 2, 4, 6, 8, 10, 15, 20, 25, 30, 45, 60, 75, 90, 120, 150, and 180 min after the injections. The aims were to determine the appropriate dose of ACTH for adrenal stimulation tests, to define the dose level of ACTH which elicited a maximal cortisol response and to describe the range of blood cortisol concentrations for reference when evaluating the stress responses of reindeer. The mean plasma cortisol concentrations (s.e.) at the zero sample times (t 0 ) of the control and the ACTH treatments varied between 93·4 (11·8) and 132·5 (18·1) nmol/l. The total plasma cortisol response (area under curve, AUC, nmol/l × min) increased with increasing dose of ACTH ( P < 0·001). The AUC of the control treatment was significantly smaller than of the ACTH treatments ( P < 0·001). The highest dose of ACTH (500 μg) gave a significantly bigger AUC than the lowest dose (100 μg) ( P = 0·008). The maximal plasma cortisol concentrations (CMAX) were achieved within 60 min of the ACTH injections. The ranges of individual CMAX were 59·0 to 136·8 nmol/l for the control treatment, and 110·0 to 252·0, 152·0 to 247·5 and 135·1 to 257·1 nmol/l for 100, 250 and 500 μg ACTH, respectively. The difference in CMAX was significant between the control treatment and the ACTH treatments ( P < 0·001) but not between the different doses of ACTH. The plasma cortisol concentrations at the end of the observation period at t180 were not significantly affected by the ACTH treatment ( P > 0·05). In conclusion, the 100-μg dose of ACTH was sufficient to produce a significant cortisol response compared with the control treatment. Increasing the dose did not increase the maximal response, but tended to elongate the response profile. The blood sampling frequency used in the study was found suitable for detection of the cortisol response in reindeer. |
format |
Article in Journal/Newspaper |
author |
Säkkinen, H. Tornberg, J. Goddard, P. J. Eloranta, E. Dahl, E. Ropstad, E. Saarela, S. |
author_facet |
Säkkinen, H. Tornberg, J. Goddard, P. J. Eloranta, E. Dahl, E. Ropstad, E. Saarela, S. |
author_sort |
Säkkinen, H. |
title |
Adrenal responsiveness of reindeer ( Rangifer tarandus tarandus ) to intravenously administered ACTH |
title_short |
Adrenal responsiveness of reindeer ( Rangifer tarandus tarandus ) to intravenously administered ACTH |
title_full |
Adrenal responsiveness of reindeer ( Rangifer tarandus tarandus ) to intravenously administered ACTH |
title_fullStr |
Adrenal responsiveness of reindeer ( Rangifer tarandus tarandus ) to intravenously administered ACTH |
title_full_unstemmed |
Adrenal responsiveness of reindeer ( Rangifer tarandus tarandus ) to intravenously administered ACTH |
title_sort |
adrenal responsiveness of reindeer ( rangifer tarandus tarandus ) to intravenously administered acth |
publisher |
Cambridge University Press (CUP) |
publishDate |
2005 |
url |
http://dx.doi.org/10.1079/asc41870399 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S1357729805000962 |
genre |
Rangifer tarandus |
genre_facet |
Rangifer tarandus |
op_source |
Animal Science volume 81, issue 3, page 399-402 ISSN 1357-7298 1748-748X |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1079/asc41870399 |
container_title |
Animal Science |
container_volume |
81 |
container_issue |
3 |
container_start_page |
399 |
op_container_end_page |
402 |
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1766175673959841792 |