Chloroplast Ultrastructure And Absorption Properties Of The Alga Phaeocystis Antarctica Karsten: A Qualitative Study Using Electron Tomography
Understanding the light-harvesting properties of algae and higher plants are a fundamental topic in photosynthesis research. Much oceanographic research has focused on characterizing the in vivo chlorophyll-specific absorption coefficient, a* ph (λ) in phytoplankton because it serves as an input var...
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1999
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Online Access: | http://dx.doi.org/10.1017/s1431927600019619 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S1431927600019619 |
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crcambridgeupr:10.1017/s1431927600019619 2023-05-15T14:06:25+02:00 Chloroplast Ultrastructure And Absorption Properties Of The Alga Phaeocystis Antarctica Karsten: A Qualitative Study Using Electron Tomography Moisan, Tiffany A. Ellisman, Mark Sosinsky, Gina 1999 http://dx.doi.org/10.1017/s1431927600019619 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S1431927600019619 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Microscopy and Microanalysis volume 5, issue S2, page 1258-1259 ISSN 1431-9276 1435-8115 Instrumentation journal-article 1999 crcambridgeupr https://doi.org/10.1017/s1431927600019619 2022-04-07T08:01:45Z Understanding the light-harvesting properties of algae and higher plants are a fundamental topic in photosynthesis research. Much oceanographic research has focused on characterizing the in vivo chlorophyll-specific absorption coefficient, a* ph (λ) in phytoplankton because it serves as an input variable for bio-optical modeling of photosynthesis using remote sensing instrumentation such as moorings, drifters, and satellites. Values of a* ph (λ) vary spectrally and the magnitude depends on accessory pigments, photo-protective pigments, and pigment packaging effects. Several studies have shown that the contribution of cellular characteristics to a* ph (λ) varies with growth conditions including temperature, light, and nutrients. It has been shown that a* ph (λ) values in Phaeocystis vary predictably at 4°C over light intensities under light limitation. Phaeocystis demonstrated significant pigment package effects that depended on single cell diameter and thylakoid membrane stacking. Using thick sections obtained from fixed and embedded cultures of colonial P. antarctica , we calculated tomographic reconstructions of individual chloroplasts under light-limiting conditions for net photosynthesis in order to gain an understanding of the continuity of thylakoid membranes and understand the spatial relationship between the pyrenoid, the starch containing organelle, and thylakoid membranes. Article in Journal/Newspaper Antarc* Antarctica Cambridge University Press (via Crossref) Microscopy and Microanalysis 5 S2 1258 1259 |
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Instrumentation |
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Instrumentation Moisan, Tiffany A. Ellisman, Mark Sosinsky, Gina Chloroplast Ultrastructure And Absorption Properties Of The Alga Phaeocystis Antarctica Karsten: A Qualitative Study Using Electron Tomography |
topic_facet |
Instrumentation |
description |
Understanding the light-harvesting properties of algae and higher plants are a fundamental topic in photosynthesis research. Much oceanographic research has focused on characterizing the in vivo chlorophyll-specific absorption coefficient, a* ph (λ) in phytoplankton because it serves as an input variable for bio-optical modeling of photosynthesis using remote sensing instrumentation such as moorings, drifters, and satellites. Values of a* ph (λ) vary spectrally and the magnitude depends on accessory pigments, photo-protective pigments, and pigment packaging effects. Several studies have shown that the contribution of cellular characteristics to a* ph (λ) varies with growth conditions including temperature, light, and nutrients. It has been shown that a* ph (λ) values in Phaeocystis vary predictably at 4°C over light intensities under light limitation. Phaeocystis demonstrated significant pigment package effects that depended on single cell diameter and thylakoid membrane stacking. Using thick sections obtained from fixed and embedded cultures of colonial P. antarctica , we calculated tomographic reconstructions of individual chloroplasts under light-limiting conditions for net photosynthesis in order to gain an understanding of the continuity of thylakoid membranes and understand the spatial relationship between the pyrenoid, the starch containing organelle, and thylakoid membranes. |
format |
Article in Journal/Newspaper |
author |
Moisan, Tiffany A. Ellisman, Mark Sosinsky, Gina |
author_facet |
Moisan, Tiffany A. Ellisman, Mark Sosinsky, Gina |
author_sort |
Moisan, Tiffany A. |
title |
Chloroplast Ultrastructure And Absorption Properties Of The Alga Phaeocystis Antarctica Karsten: A Qualitative Study Using Electron Tomography |
title_short |
Chloroplast Ultrastructure And Absorption Properties Of The Alga Phaeocystis Antarctica Karsten: A Qualitative Study Using Electron Tomography |
title_full |
Chloroplast Ultrastructure And Absorption Properties Of The Alga Phaeocystis Antarctica Karsten: A Qualitative Study Using Electron Tomography |
title_fullStr |
Chloroplast Ultrastructure And Absorption Properties Of The Alga Phaeocystis Antarctica Karsten: A Qualitative Study Using Electron Tomography |
title_full_unstemmed |
Chloroplast Ultrastructure And Absorption Properties Of The Alga Phaeocystis Antarctica Karsten: A Qualitative Study Using Electron Tomography |
title_sort |
chloroplast ultrastructure and absorption properties of the alga phaeocystis antarctica karsten: a qualitative study using electron tomography |
publisher |
Cambridge University Press (CUP) |
publishDate |
1999 |
url |
http://dx.doi.org/10.1017/s1431927600019619 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S1431927600019619 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Microscopy and Microanalysis volume 5, issue S2, page 1258-1259 ISSN 1431-9276 1435-8115 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s1431927600019619 |
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Microscopy and Microanalysis |
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5 |
container_issue |
S2 |
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1258 |
op_container_end_page |
1259 |
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1766278117541806080 |