The ability of whale haploid spermatogenic cells to induce calcium oscillations and its relevance to oocyte activation
Summary Interspecies microinsemination assay was applied to examine the ability of minke whale haploid spermatogenic cells to induce Ca 2+ oscillations and oocyte activation. Populations of round spermatids (RS), early-stage elongating spermatids (e-ES), late-stage elongating spermatids (1-ES) and t...
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crcambridgeupr:10.1017/s0967199406004047 2023-06-11T04:06:48+02:00 The ability of whale haploid spermatogenic cells to induce calcium oscillations and its relevance to oocyte activation Amemiya, K. Hirabayashi, M. Ishikawa, H. Fukui, Y. Hochi, S. 2007 http://dx.doi.org/10.1017/s0967199406004047 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0967199406004047 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Zygote volume 15, issue 2, page 103-108 ISSN 0967-1994 1469-8730 Cell Biology Developmental Biology journal-article 2007 crcambridgeupr https://doi.org/10.1017/s0967199406004047 2023-05-01T18:20:49Z Summary Interspecies microinsemination assay was applied to examine the ability of minke whale haploid spermatogenic cells to induce Ca 2+ oscillations and oocyte activation. Populations of round spermatids (RS), early-stage elongating spermatids (e-ES), late-stage elongating spermatids (1-ES) and testicular spermatozoa (TS) were cryopreserved in the presence of 7.5% glycerol on board ship in the Antarctic Ocean. Repetitive increases of intracellular Ca 2+ concentration occurred in 0, 65, 81 and 96% of BDF1 mouse oocytes injected with the postthaw RS, e-ES, 1-ES and TS, respectively. A normal pattern of the Ca 2+ oscillations was observed in 26–47% of the responding oocytes. Most oocytes that exhibited Ca 2+ oscillations, regardless of the oscillation pattern, resumed meiosis (83–94%). These results indicate that whale spermatogenic cells acquire SOAF activity, which is closely related to their Ca 2+ oscillation-inducing ability at the relatively early stage of spermiogenesis. Article in Journal/Newspaper Antarc* Antarctic Antarctic Ocean minke whale Cambridge University Press (via Crossref) Antarctic Antarctic Ocean The Antarctic Zygote 15 2 103 108 |
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Cambridge University Press (via Crossref) |
op_collection_id |
crcambridgeupr |
language |
English |
topic |
Cell Biology Developmental Biology |
spellingShingle |
Cell Biology Developmental Biology Amemiya, K. Hirabayashi, M. Ishikawa, H. Fukui, Y. Hochi, S. The ability of whale haploid spermatogenic cells to induce calcium oscillations and its relevance to oocyte activation |
topic_facet |
Cell Biology Developmental Biology |
description |
Summary Interspecies microinsemination assay was applied to examine the ability of minke whale haploid spermatogenic cells to induce Ca 2+ oscillations and oocyte activation. Populations of round spermatids (RS), early-stage elongating spermatids (e-ES), late-stage elongating spermatids (1-ES) and testicular spermatozoa (TS) were cryopreserved in the presence of 7.5% glycerol on board ship in the Antarctic Ocean. Repetitive increases of intracellular Ca 2+ concentration occurred in 0, 65, 81 and 96% of BDF1 mouse oocytes injected with the postthaw RS, e-ES, 1-ES and TS, respectively. A normal pattern of the Ca 2+ oscillations was observed in 26–47% of the responding oocytes. Most oocytes that exhibited Ca 2+ oscillations, regardless of the oscillation pattern, resumed meiosis (83–94%). These results indicate that whale spermatogenic cells acquire SOAF activity, which is closely related to their Ca 2+ oscillation-inducing ability at the relatively early stage of spermiogenesis. |
format |
Article in Journal/Newspaper |
author |
Amemiya, K. Hirabayashi, M. Ishikawa, H. Fukui, Y. Hochi, S. |
author_facet |
Amemiya, K. Hirabayashi, M. Ishikawa, H. Fukui, Y. Hochi, S. |
author_sort |
Amemiya, K. |
title |
The ability of whale haploid spermatogenic cells to induce calcium oscillations and its relevance to oocyte activation |
title_short |
The ability of whale haploid spermatogenic cells to induce calcium oscillations and its relevance to oocyte activation |
title_full |
The ability of whale haploid spermatogenic cells to induce calcium oscillations and its relevance to oocyte activation |
title_fullStr |
The ability of whale haploid spermatogenic cells to induce calcium oscillations and its relevance to oocyte activation |
title_full_unstemmed |
The ability of whale haploid spermatogenic cells to induce calcium oscillations and its relevance to oocyte activation |
title_sort |
ability of whale haploid spermatogenic cells to induce calcium oscillations and its relevance to oocyte activation |
publisher |
Cambridge University Press (CUP) |
publishDate |
2007 |
url |
http://dx.doi.org/10.1017/s0967199406004047 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0967199406004047 |
geographic |
Antarctic Antarctic Ocean The Antarctic |
geographic_facet |
Antarctic Antarctic Ocean The Antarctic |
genre |
Antarc* Antarctic Antarctic Ocean minke whale |
genre_facet |
Antarc* Antarctic Antarctic Ocean minke whale |
op_source |
Zygote volume 15, issue 2, page 103-108 ISSN 0967-1994 1469-8730 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0967199406004047 |
container_title |
Zygote |
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15 |
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2 |
container_start_page |
103 |
op_container_end_page |
108 |
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1768378967425810432 |