The ability of whale haploid spermatogenic cells to induce calcium oscillations and its relevance to oocyte activation

Summary Interspecies microinsemination assay was applied to examine the ability of minke whale haploid spermatogenic cells to induce Ca 2+ oscillations and oocyte activation. Populations of round spermatids (RS), early-stage elongating spermatids (e-ES), late-stage elongating spermatids (1-ES) and t...

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Bibliographic Details
Published in:Zygote
Main Authors: Amemiya, K., Hirabayashi, M., Ishikawa, H., Fukui, Y., Hochi, S.
Format: Article in Journal/Newspaper
Language:English
Published: Cambridge University Press (CUP) 2007
Subjects:
Cell Biology
Developmental Biology
Antarctic
Antarctic Ocean
The Antarctic
Antarc*
minke whale
Online Access:http://dx.doi.org/10.1017/s0967199406004047
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0967199406004047
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Summary:Summary Interspecies microinsemination assay was applied to examine the ability of minke whale haploid spermatogenic cells to induce Ca 2+ oscillations and oocyte activation. Populations of round spermatids (RS), early-stage elongating spermatids (e-ES), late-stage elongating spermatids (1-ES) and testicular spermatozoa (TS) were cryopreserved in the presence of 7.5% glycerol on board ship in the Antarctic Ocean. Repetitive increases of intracellular Ca 2+ concentration occurred in 0, 65, 81 and 96% of BDF1 mouse oocytes injected with the postthaw RS, e-ES, 1-ES and TS, respectively. A normal pattern of the Ca 2+ oscillations was observed in 26–47% of the responding oocytes. Most oocytes that exhibited Ca 2+ oscillations, regardless of the oscillation pattern, resumed meiosis (83–94%). These results indicate that whale spermatogenic cells acquire SOAF activity, which is closely related to their Ca 2+ oscillation-inducing ability at the relatively early stage of spermiogenesis.

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