Characterization of a hydrocarbon degrading psychrotrophic Antarctic bacterium
Antarctic soil chronically exposed to gas-oil was analysed in order to isolate and study the growth conditions of hydrocarbon degrading bacteria. Soil samples taken near the shoreline in Jubany Station (King George Island, South Shetland Islands) were used as inoculum in liquid culture media with cr...
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1997
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Online Access: | http://dx.doi.org/10.1017/s0954102097000199 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102097000199 |
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crcambridgeupr:10.1017/s0954102097000199 2024-03-03T08:37:25+00:00 Characterization of a hydrocarbon degrading psychrotrophic Antarctic bacterium Cormack, Walter P. Mac Fraile, Elda R. 1997 http://dx.doi.org/10.1017/s0954102097000199 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102097000199 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 9, issue 2, page 150-155 ISSN 0954-1020 1365-2079 Geology Ecology, Evolution, Behavior and Systematics Oceanography journal-article 1997 crcambridgeupr https://doi.org/10.1017/s0954102097000199 2024-02-08T08:34:50Z Antarctic soil chronically exposed to gas-oil was analysed in order to isolate and study the growth conditions of hydrocarbon degrading bacteria. Soil samples taken near the shoreline in Jubany Station (King George Island, South Shetland Islands) were used as inoculum in liquid culture media with crude oil as sole carbon source. A psychrotrophic Acinetobacter strain was isolated and selected for further investigations. Effects were studied of temperature, initial pH, NaCl concentration and different chemical structure of the hydrocarbon on growth. Degradation rate was determined with n-dodecane and n-hexadecane. Growth of Acinetobacter ADH-1 showed no differences at an initial pH of 7.0, 7.5 and 8.0. Optimum temperature ranged between 25–30°C but the strain was capable of growing on n-dodecane at 4°C. Growth was observed in the presence of 3.5% NaCl. A decrease in the surface tension values was observed in the culture broth during the first 20 h of incubation (from 68 din cm −1 to 31 din cm −1 ). This proved to be related to the cellular fraction of the culture. The study shows that Acinetobacter ADH-1 is a psychrotrophic bacteria able to grow with hydrocarbons as sole carbon and energy source and could be potentially useful to design bioremediation processes in temperate and cold climate areas. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science King George Island South Shetland Islands Cambridge University Press Antarctic King George Island South Shetland Islands Antarctic Science 9 2 150 155 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
topic |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
spellingShingle |
Geology Ecology, Evolution, Behavior and Systematics Oceanography Cormack, Walter P. Mac Fraile, Elda R. Characterization of a hydrocarbon degrading psychrotrophic Antarctic bacterium |
topic_facet |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
description |
Antarctic soil chronically exposed to gas-oil was analysed in order to isolate and study the growth conditions of hydrocarbon degrading bacteria. Soil samples taken near the shoreline in Jubany Station (King George Island, South Shetland Islands) were used as inoculum in liquid culture media with crude oil as sole carbon source. A psychrotrophic Acinetobacter strain was isolated and selected for further investigations. Effects were studied of temperature, initial pH, NaCl concentration and different chemical structure of the hydrocarbon on growth. Degradation rate was determined with n-dodecane and n-hexadecane. Growth of Acinetobacter ADH-1 showed no differences at an initial pH of 7.0, 7.5 and 8.0. Optimum temperature ranged between 25–30°C but the strain was capable of growing on n-dodecane at 4°C. Growth was observed in the presence of 3.5% NaCl. A decrease in the surface tension values was observed in the culture broth during the first 20 h of incubation (from 68 din cm −1 to 31 din cm −1 ). This proved to be related to the cellular fraction of the culture. The study shows that Acinetobacter ADH-1 is a psychrotrophic bacteria able to grow with hydrocarbons as sole carbon and energy source and could be potentially useful to design bioremediation processes in temperate and cold climate areas. |
format |
Article in Journal/Newspaper |
author |
Cormack, Walter P. Mac Fraile, Elda R. |
author_facet |
Cormack, Walter P. Mac Fraile, Elda R. |
author_sort |
Cormack, Walter P. Mac |
title |
Characterization of a hydrocarbon degrading psychrotrophic Antarctic bacterium |
title_short |
Characterization of a hydrocarbon degrading psychrotrophic Antarctic bacterium |
title_full |
Characterization of a hydrocarbon degrading psychrotrophic Antarctic bacterium |
title_fullStr |
Characterization of a hydrocarbon degrading psychrotrophic Antarctic bacterium |
title_full_unstemmed |
Characterization of a hydrocarbon degrading psychrotrophic Antarctic bacterium |
title_sort |
characterization of a hydrocarbon degrading psychrotrophic antarctic bacterium |
publisher |
Cambridge University Press (CUP) |
publishDate |
1997 |
url |
http://dx.doi.org/10.1017/s0954102097000199 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102097000199 |
geographic |
Antarctic King George Island South Shetland Islands |
geographic_facet |
Antarctic King George Island South Shetland Islands |
genre |
Antarc* Antarctic Antarctic Science King George Island South Shetland Islands |
genre_facet |
Antarc* Antarctic Antarctic Science King George Island South Shetland Islands |
op_source |
Antarctic Science volume 9, issue 2, page 150-155 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102097000199 |
container_title |
Antarctic Science |
container_volume |
9 |
container_issue |
2 |
container_start_page |
150 |
op_container_end_page |
155 |
_version_ |
1792498773351464960 |