Estimation of neutral lipid levels in Antarctic sea ice microalgae by nile red fluorescence
The fluorescent neutral lipid stain, nile red, was used to examine cell-specific neutral lipid levels in natural assemblages of Antarctic sea ice microalgae. Neutral lipid:chlorophyll, neutral lipid:particulate carbon (PC) and neutral lipid:particulate nitrogen (PN) ratios were highest in communitie...
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Cambridge University Press (CUP)
1990
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Online Access: | http://dx.doi.org/10.1017/s0954102090000190 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102090000190 |
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crcambridgeupr:10.1017/s0954102090000190 2024-09-15T17:48:07+00:00 Estimation of neutral lipid levels in Antarctic sea ice microalgae by nile red fluorescence Priscu, John C. Priscu, Linda R. Palmisano, Anna C. Sullivan, Cornelius W. 1990 http://dx.doi.org/10.1017/s0954102090000190 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102090000190 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 2, issue 2, page 149-155 ISSN 0954-1020 1365-2079 journal-article 1990 crcambridgeupr https://doi.org/10.1017/s0954102090000190 2024-07-17T04:01:33Z The fluorescent neutral lipid stain, nile red, was used to examine cell-specific neutral lipid levels in natural assemblages of Antarctic sea ice microalgae. Neutral lipid:chlorophyll, neutral lipid:particulate carbon (PC) and neutral lipid:particulate nitrogen (PN) ratios were highest in communities dominated by Nitzschia spp. and Navicula glaciei van Heurck. The lowest specific neutral lipid content was estimated in the congelation ice samples dominated by the diatom Amphiprora spp., and in surface assemblages dominated by Phaeocystis pouchetii Hariot and the dinoflagellate Gymnodinium sp. Scatter plots of neutral lipid on PC and PN, which included data from all assemblages, showed that assemblages dominated by P. pouchetii and Amphiprora spp. clustered near the origin reflecting their relatively lower specific neutral lipid levels, compared with assemblages dominated by N. glaciei and Nitzschia spp. Cellular PC:PN was significantly (P<0.001) lower in microalgae inhabiting surface melt pools or tide cracks compared to those associated with congelation or platelet ice. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Sea ice Cambridge University Press Antarctic Science 2 2 149 155 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
description |
The fluorescent neutral lipid stain, nile red, was used to examine cell-specific neutral lipid levels in natural assemblages of Antarctic sea ice microalgae. Neutral lipid:chlorophyll, neutral lipid:particulate carbon (PC) and neutral lipid:particulate nitrogen (PN) ratios were highest in communities dominated by Nitzschia spp. and Navicula glaciei van Heurck. The lowest specific neutral lipid content was estimated in the congelation ice samples dominated by the diatom Amphiprora spp., and in surface assemblages dominated by Phaeocystis pouchetii Hariot and the dinoflagellate Gymnodinium sp. Scatter plots of neutral lipid on PC and PN, which included data from all assemblages, showed that assemblages dominated by P. pouchetii and Amphiprora spp. clustered near the origin reflecting their relatively lower specific neutral lipid levels, compared with assemblages dominated by N. glaciei and Nitzschia spp. Cellular PC:PN was significantly (P<0.001) lower in microalgae inhabiting surface melt pools or tide cracks compared to those associated with congelation or platelet ice. |
format |
Article in Journal/Newspaper |
author |
Priscu, John C. Priscu, Linda R. Palmisano, Anna C. Sullivan, Cornelius W. |
spellingShingle |
Priscu, John C. Priscu, Linda R. Palmisano, Anna C. Sullivan, Cornelius W. Estimation of neutral lipid levels in Antarctic sea ice microalgae by nile red fluorescence |
author_facet |
Priscu, John C. Priscu, Linda R. Palmisano, Anna C. Sullivan, Cornelius W. |
author_sort |
Priscu, John C. |
title |
Estimation of neutral lipid levels in Antarctic sea ice microalgae by nile red fluorescence |
title_short |
Estimation of neutral lipid levels in Antarctic sea ice microalgae by nile red fluorescence |
title_full |
Estimation of neutral lipid levels in Antarctic sea ice microalgae by nile red fluorescence |
title_fullStr |
Estimation of neutral lipid levels in Antarctic sea ice microalgae by nile red fluorescence |
title_full_unstemmed |
Estimation of neutral lipid levels in Antarctic sea ice microalgae by nile red fluorescence |
title_sort |
estimation of neutral lipid levels in antarctic sea ice microalgae by nile red fluorescence |
publisher |
Cambridge University Press (CUP) |
publishDate |
1990 |
url |
http://dx.doi.org/10.1017/s0954102090000190 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102090000190 |
genre |
Antarc* Antarctic Antarctic Science Sea ice |
genre_facet |
Antarc* Antarctic Antarctic Science Sea ice |
op_source |
Antarctic Science volume 2, issue 2, page 149-155 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102090000190 |
container_title |
Antarctic Science |
container_volume |
2 |
container_issue |
2 |
container_start_page |
149 |
op_container_end_page |
155 |
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1810289250183151616 |