Antibiotic resistance genes and taxa analysis from mat and planktonic microbiomes of Antarctic perennial ice-covered Lake Fryxell and Lake Bonney
Abstract The perennial ice-covered lakes of the Antarctic McMurdo Dry Valleys harbour oligotrophic microbial communities that are separated geographically from other aquatic systems. Their microbiomes include planktonic microbes as well as lift-off mat communities that emerge from the ice. We used t...
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crcambridgeupr:10.1017/s0954102022000360 2024-03-03T08:37:32+00:00 Antibiotic resistance genes and taxa analysis from mat and planktonic microbiomes of Antarctic perennial ice-covered Lake Fryxell and Lake Bonney Tallada, Sheetal Hall, Grant Barich, Daniel Morgan-Kiss, Rachael M. Slonczewski, Joan L. 2022 http://dx.doi.org/10.1017/s0954102022000360 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102022000360 en eng Cambridge University Press (CUP) http://creativecommons.org/licenses/by/4.0/ Antarctic Science volume 34, issue 6, page 408-422 ISSN 0954-1020 1365-2079 Geology Ecology, Evolution, Behavior and Systematics Oceanography journal-article 2022 crcambridgeupr https://doi.org/10.1017/s0954102022000360 2024-02-08T08:43:23Z Abstract The perennial ice-covered lakes of the Antarctic McMurdo Dry Valleys harbour oligotrophic microbial communities that are separated geographically from other aquatic systems. Their microbiomes include planktonic microbes as well as lift-off mat communities that emerge from the ice. We used the ShortBRED protein family profiler to quantify the antibiotic resistance genes (ARGs) from metagenomes of lift-off mats emerging from ice and from filtered water samples of Lake Fryxell and Lake Bonney. The overall proportion of ARG hits was similar to that found in temperate-zone rural ponds with moderate human inputs. Specific ARGs showed distinct distributions for the two lakes and for mat vs planktonic sources. Metagenomic taxa distributions showed that mat phototrophs consisted mainly of cyanobacteria or Betaproteobacteria, whereas the water column phototrophs were mainly protists. An enrichment culture of the Betaproteobacterium Rhodoferax antarcticus from a Lake Fryxell mat sample showed an unusual mat-forming phenotype not previously reported for this species. Its genome showed no ARGs associated with Betaproteobacteria but had ARGs consistent with a minor Pseudomonas component. The Antarctic lake mats and water showed specific ARGs distinctive to the mat and water sources, but overall ARG levels were similar to those of temperate water bodies with moderate human inputs. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science antarcticus McMurdo Dry Valleys Cambridge University Press Antarctic The Antarctic McMurdo Dry Valleys Bonney ENVELOPE(162.417,162.417,-77.717,-77.717) Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) Lake Bonney ENVELOPE(-25.588,-25.588,-80.361,-80.361) Lake Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) Antarctic Science 34 6 408 422 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
topic |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
spellingShingle |
Geology Ecology, Evolution, Behavior and Systematics Oceanography Tallada, Sheetal Hall, Grant Barich, Daniel Morgan-Kiss, Rachael M. Slonczewski, Joan L. Antibiotic resistance genes and taxa analysis from mat and planktonic microbiomes of Antarctic perennial ice-covered Lake Fryxell and Lake Bonney |
topic_facet |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
description |
Abstract The perennial ice-covered lakes of the Antarctic McMurdo Dry Valleys harbour oligotrophic microbial communities that are separated geographically from other aquatic systems. Their microbiomes include planktonic microbes as well as lift-off mat communities that emerge from the ice. We used the ShortBRED protein family profiler to quantify the antibiotic resistance genes (ARGs) from metagenomes of lift-off mats emerging from ice and from filtered water samples of Lake Fryxell and Lake Bonney. The overall proportion of ARG hits was similar to that found in temperate-zone rural ponds with moderate human inputs. Specific ARGs showed distinct distributions for the two lakes and for mat vs planktonic sources. Metagenomic taxa distributions showed that mat phototrophs consisted mainly of cyanobacteria or Betaproteobacteria, whereas the water column phototrophs were mainly protists. An enrichment culture of the Betaproteobacterium Rhodoferax antarcticus from a Lake Fryxell mat sample showed an unusual mat-forming phenotype not previously reported for this species. Its genome showed no ARGs associated with Betaproteobacteria but had ARGs consistent with a minor Pseudomonas component. The Antarctic lake mats and water showed specific ARGs distinctive to the mat and water sources, but overall ARG levels were similar to those of temperate water bodies with moderate human inputs. |
format |
Article in Journal/Newspaper |
author |
Tallada, Sheetal Hall, Grant Barich, Daniel Morgan-Kiss, Rachael M. Slonczewski, Joan L. |
author_facet |
Tallada, Sheetal Hall, Grant Barich, Daniel Morgan-Kiss, Rachael M. Slonczewski, Joan L. |
author_sort |
Tallada, Sheetal |
title |
Antibiotic resistance genes and taxa analysis from mat and planktonic microbiomes of Antarctic perennial ice-covered Lake Fryxell and Lake Bonney |
title_short |
Antibiotic resistance genes and taxa analysis from mat and planktonic microbiomes of Antarctic perennial ice-covered Lake Fryxell and Lake Bonney |
title_full |
Antibiotic resistance genes and taxa analysis from mat and planktonic microbiomes of Antarctic perennial ice-covered Lake Fryxell and Lake Bonney |
title_fullStr |
Antibiotic resistance genes and taxa analysis from mat and planktonic microbiomes of Antarctic perennial ice-covered Lake Fryxell and Lake Bonney |
title_full_unstemmed |
Antibiotic resistance genes and taxa analysis from mat and planktonic microbiomes of Antarctic perennial ice-covered Lake Fryxell and Lake Bonney |
title_sort |
antibiotic resistance genes and taxa analysis from mat and planktonic microbiomes of antarctic perennial ice-covered lake fryxell and lake bonney |
publisher |
Cambridge University Press (CUP) |
publishDate |
2022 |
url |
http://dx.doi.org/10.1017/s0954102022000360 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102022000360 |
long_lat |
ENVELOPE(162.417,162.417,-77.717,-77.717) ENVELOPE(163.183,163.183,-77.617,-77.617) ENVELOPE(-25.588,-25.588,-80.361,-80.361) ENVELOPE(163.183,163.183,-77.617,-77.617) |
geographic |
Antarctic The Antarctic McMurdo Dry Valleys Bonney Fryxell Lake Bonney Lake Fryxell |
geographic_facet |
Antarctic The Antarctic McMurdo Dry Valleys Bonney Fryxell Lake Bonney Lake Fryxell |
genre |
Antarc* Antarctic Antarctic Science antarcticus McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctic Antarctic Science antarcticus McMurdo Dry Valleys |
op_source |
Antarctic Science volume 34, issue 6, page 408-422 ISSN 0954-1020 1365-2079 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1017/s0954102022000360 |
container_title |
Antarctic Science |
container_volume |
34 |
container_issue |
6 |
container_start_page |
408 |
op_container_end_page |
422 |
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1792499500122636288 |