Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica
Abstract Virus diversity in Antarctic biotopes remains understudied. Here, we describe bacteriophages isolated from terrestrial environments, provide data on their natural bacterial hosts and study phage-host systems. Six bacterial isolates (FCKU 539, FCKU 533, FCKU 534, FCKU 538, FCKU 542 and FCKU...
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Cambridge University Press (CUP)
2021
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Online Access: | http://dx.doi.org/10.1017/s0954102021000572 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102021000572 |
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crcambridgeupr:10.1017/s0954102021000572 2024-03-03T08:38:56+00:00 Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica Holovan, Viktoriia Andriichuk, Olena Budzanivska, Irena Zelena, Pavlina Kondratiuk, Tetiana Shevchenko, Oleksiy National Antarctic Scientific Center (NASC) of the Ministry of Education and Science of Ukraine 2021 http://dx.doi.org/10.1017/s0954102021000572 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102021000572 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 34, issue 2, page 120-136 ISSN 0954-1020 1365-2079 Geology Ecology, Evolution, Behavior and Systematics Oceanography journal-article 2021 crcambridgeupr https://doi.org/10.1017/s0954102021000572 2024-02-08T08:38:09Z Abstract Virus diversity in Antarctic biotopes remains understudied. Here, we describe bacteriophages isolated from terrestrial environments, provide data on their natural bacterial hosts and study phage-host systems. Six bacterial isolates (FCKU 539, FCKU 533, FCKU 534, FCKU 538, FCKU 542 and FCKU 540) were recovered and characterized. Isolated bacteria belonged to Pseudomonas genus ( Pseudomonas sp., Pseudomonas fluorescens , Pseudomonas putida ) with optimal cultivation temperatures of 16–28°C. These bacteria and previously described Bacillus subtilis FCKU 537 were used for analysing virus-host interactions. Six lytic phages were isolated and named P. fluorescens Antarctic virus 1 (PFAV1), P. fluorescens Antarctic virus 2 (PFAV2), P. fluorescens Antarctic virus 3 (PFAV3), P. putida Antarctic virus 4 (PPAV4), Pseudomonas sp. Antarctic virus 5 (PSAV5) and B. subtilis Antarctic virus 6 (BSAV6) in relation to their natural hosts. According to electron microscopy data, these phages belonged to Caudovirales order. Cross-inoculation demonstrated high specificity of all Antarctic phages, which infected only their initial hosts at moderate temperatures. PFAV2 and PFAV3 phages also infected laboratory Pseudomonas savastanoi and P. fluorescens isolates. This paper adds new data on the occurrence and diversity of viruses and their respective bacterial hosts in soil biotopes of Antarctica. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Antarctica Cambridge University Press Antarctic Antarctic Science 1 17 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
topic |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
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Geology Ecology, Evolution, Behavior and Systematics Oceanography Holovan, Viktoriia Andriichuk, Olena Budzanivska, Irena Zelena, Pavlina Kondratiuk, Tetiana Shevchenko, Oleksiy Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica |
topic_facet |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
description |
Abstract Virus diversity in Antarctic biotopes remains understudied. Here, we describe bacteriophages isolated from terrestrial environments, provide data on their natural bacterial hosts and study phage-host systems. Six bacterial isolates (FCKU 539, FCKU 533, FCKU 534, FCKU 538, FCKU 542 and FCKU 540) were recovered and characterized. Isolated bacteria belonged to Pseudomonas genus ( Pseudomonas sp., Pseudomonas fluorescens , Pseudomonas putida ) with optimal cultivation temperatures of 16–28°C. These bacteria and previously described Bacillus subtilis FCKU 537 were used for analysing virus-host interactions. Six lytic phages were isolated and named P. fluorescens Antarctic virus 1 (PFAV1), P. fluorescens Antarctic virus 2 (PFAV2), P. fluorescens Antarctic virus 3 (PFAV3), P. putida Antarctic virus 4 (PPAV4), Pseudomonas sp. Antarctic virus 5 (PSAV5) and B. subtilis Antarctic virus 6 (BSAV6) in relation to their natural hosts. According to electron microscopy data, these phages belonged to Caudovirales order. Cross-inoculation demonstrated high specificity of all Antarctic phages, which infected only their initial hosts at moderate temperatures. PFAV2 and PFAV3 phages also infected laboratory Pseudomonas savastanoi and P. fluorescens isolates. This paper adds new data on the occurrence and diversity of viruses and their respective bacterial hosts in soil biotopes of Antarctica. |
author2 |
National Antarctic Scientific Center (NASC) of the Ministry of Education and Science of Ukraine |
format |
Article in Journal/Newspaper |
author |
Holovan, Viktoriia Andriichuk, Olena Budzanivska, Irena Zelena, Pavlina Kondratiuk, Tetiana Shevchenko, Oleksiy |
author_facet |
Holovan, Viktoriia Andriichuk, Olena Budzanivska, Irena Zelena, Pavlina Kondratiuk, Tetiana Shevchenko, Oleksiy |
author_sort |
Holovan, Viktoriia |
title |
Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica |
title_short |
Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica |
title_full |
Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica |
title_fullStr |
Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica |
title_full_unstemmed |
Bacteriophages and their microbial hosts in terrestrial biotopes of Antarctica |
title_sort |
bacteriophages and their microbial hosts in terrestrial biotopes of antarctica |
publisher |
Cambridge University Press (CUP) |
publishDate |
2021 |
url |
http://dx.doi.org/10.1017/s0954102021000572 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102021000572 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Science Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Science Antarctica |
op_source |
Antarctic Science volume 34, issue 2, page 120-136 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102021000572 |
container_title |
Antarctic Science |
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1 |
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17 |
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1792494322387517440 |