Exploring the plant environmental DNA diversity in soil from two sites on Deception Island (Antarctica, South Shetland Islands) using metabarcoding
Abstract The few Antarctic studies to date to have applied metabarcoding in Antarctica have primarily focused on microorganisms. In this study, for the first time, we apply high-throughput sequencing of environmental DNA to investigate the diversity of Embryophyta (Viridiplantae) DNA present in soil...
Published in: | Antarctic Science |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Cambridge University Press (CUP)
2021
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1017/s0954102021000274 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102021000274 |
id |
crcambridgeupr:10.1017/s0954102021000274 |
---|---|
record_format |
openpolar |
spelling |
crcambridgeupr:10.1017/s0954102021000274 2024-04-07T07:47:40+00:00 Exploring the plant environmental DNA diversity in soil from two sites on Deception Island (Antarctica, South Shetland Islands) using metabarcoding Carvalho-Silva, Micheline Rosa, Luiz Henrique Pinto, Otávio H.B. Da Silva, Thamar Holanda Henriques, Diego Knop Convey, Peter Câmara, Paulo E.A.S. 2021 http://dx.doi.org/10.1017/s0954102021000274 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102021000274 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 33, issue 5, page 469-478 ISSN 0954-1020 1365-2079 Geology Ecology, Evolution, Behavior and Systematics Oceanography journal-article 2021 crcambridgeupr https://doi.org/10.1017/s0954102021000274 2024-03-08T00:36:12Z Abstract The few Antarctic studies to date to have applied metabarcoding in Antarctica have primarily focused on microorganisms. In this study, for the first time, we apply high-throughput sequencing of environmental DNA to investigate the diversity of Embryophyta (Viridiplantae) DNA present in soil samples from two contrasting locations on Deception Island. The first was a relatively undisturbed site within an Antarctic Specially Protected Area at Crater Lake, and the second was a heavily human-impacted site in Whalers Bay. In samples obtained at Crater Lake, 84% of DNA reads represented fungi, 14% represented Chlorophyta and 2% represented Streptophyta, while at Whalers Bay, 79% of reads represented fungi, 20% represented Chlorophyta and < 1% represented Streptophyta, with ~1% of reads being unassigned. Among the Embryophyta we found 16 plant operational taxonomic units from three Divisions, including one Marchantiophyta, eight Bryophyta and seven Magnoliophyta. Sequences of six taxa were detected at both sampling sites, eight only at Whalers Bay and two only at Crater Lake. All of the Magnoliophyta sequences (flowering plants) represent species that are exotic to Antarctica, with most being plausibly linked to human food sources originating from local national research operator and tourism facilities. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Antarctica Deception Island South Shetland Islands Cambridge University Press Antarctic South Shetland Islands Deception Island ENVELOPE(-60.633,-60.633,-62.950,-62.950) Crater Lake ENVELOPE(-60.667,-60.667,-62.983,-62.983) Whalers Bay ENVELOPE(69.917,69.917,-49.100,-49.100) Antarctic Science 1 10 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
topic |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
spellingShingle |
Geology Ecology, Evolution, Behavior and Systematics Oceanography Carvalho-Silva, Micheline Rosa, Luiz Henrique Pinto, Otávio H.B. Da Silva, Thamar Holanda Henriques, Diego Knop Convey, Peter Câmara, Paulo E.A.S. Exploring the plant environmental DNA diversity in soil from two sites on Deception Island (Antarctica, South Shetland Islands) using metabarcoding |
topic_facet |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
description |
Abstract The few Antarctic studies to date to have applied metabarcoding in Antarctica have primarily focused on microorganisms. In this study, for the first time, we apply high-throughput sequencing of environmental DNA to investigate the diversity of Embryophyta (Viridiplantae) DNA present in soil samples from two contrasting locations on Deception Island. The first was a relatively undisturbed site within an Antarctic Specially Protected Area at Crater Lake, and the second was a heavily human-impacted site in Whalers Bay. In samples obtained at Crater Lake, 84% of DNA reads represented fungi, 14% represented Chlorophyta and 2% represented Streptophyta, while at Whalers Bay, 79% of reads represented fungi, 20% represented Chlorophyta and < 1% represented Streptophyta, with ~1% of reads being unassigned. Among the Embryophyta we found 16 plant operational taxonomic units from three Divisions, including one Marchantiophyta, eight Bryophyta and seven Magnoliophyta. Sequences of six taxa were detected at both sampling sites, eight only at Whalers Bay and two only at Crater Lake. All of the Magnoliophyta sequences (flowering plants) represent species that are exotic to Antarctica, with most being plausibly linked to human food sources originating from local national research operator and tourism facilities. |
format |
Article in Journal/Newspaper |
author |
Carvalho-Silva, Micheline Rosa, Luiz Henrique Pinto, Otávio H.B. Da Silva, Thamar Holanda Henriques, Diego Knop Convey, Peter Câmara, Paulo E.A.S. |
author_facet |
Carvalho-Silva, Micheline Rosa, Luiz Henrique Pinto, Otávio H.B. Da Silva, Thamar Holanda Henriques, Diego Knop Convey, Peter Câmara, Paulo E.A.S. |
author_sort |
Carvalho-Silva, Micheline |
title |
Exploring the plant environmental DNA diversity in soil from two sites on Deception Island (Antarctica, South Shetland Islands) using metabarcoding |
title_short |
Exploring the plant environmental DNA diversity in soil from two sites on Deception Island (Antarctica, South Shetland Islands) using metabarcoding |
title_full |
Exploring the plant environmental DNA diversity in soil from two sites on Deception Island (Antarctica, South Shetland Islands) using metabarcoding |
title_fullStr |
Exploring the plant environmental DNA diversity in soil from two sites on Deception Island (Antarctica, South Shetland Islands) using metabarcoding |
title_full_unstemmed |
Exploring the plant environmental DNA diversity in soil from two sites on Deception Island (Antarctica, South Shetland Islands) using metabarcoding |
title_sort |
exploring the plant environmental dna diversity in soil from two sites on deception island (antarctica, south shetland islands) using metabarcoding |
publisher |
Cambridge University Press (CUP) |
publishDate |
2021 |
url |
http://dx.doi.org/10.1017/s0954102021000274 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102021000274 |
long_lat |
ENVELOPE(-60.633,-60.633,-62.950,-62.950) ENVELOPE(-60.667,-60.667,-62.983,-62.983) ENVELOPE(69.917,69.917,-49.100,-49.100) |
geographic |
Antarctic South Shetland Islands Deception Island Crater Lake Whalers Bay |
geographic_facet |
Antarctic South Shetland Islands Deception Island Crater Lake Whalers Bay |
genre |
Antarc* Antarctic Antarctic Science Antarctica Deception Island South Shetland Islands |
genre_facet |
Antarc* Antarctic Antarctic Science Antarctica Deception Island South Shetland Islands |
op_source |
Antarctic Science volume 33, issue 5, page 469-478 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102021000274 |
container_title |
Antarctic Science |
container_start_page |
1 |
op_container_end_page |
10 |
_version_ |
1795674791620902912 |