Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans
Abstract Mosses have adapted to the Antarctic environment and are an ideal medium for studying plant resistance to abiotic stress. Chalcone synthase is the first committed enzyme in the flavonoid metabolic pathway, which plays an indispensable role in plant resistance to adversity. In this study, si...
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crcambridgeupr:10.1017/s0954102018000470 2024-09-15T17:41:13+00:00 Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans Yao, Xinghao Wang, Tailin Wang, Huijuan Liu, Hongwei Liu, Shenghao Zhao, Qingang Chen, Kaoshan Zhang, Pengying 2019 http://dx.doi.org/10.1017/s0954102018000470 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102018000470 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms https://www.cambridge.org/core/terms Antarctic Science volume 31, issue 1, page 23-33 ISSN 0954-1020 1365-2079 journal-article 2019 crcambridgeupr https://doi.org/10.1017/s0954102018000470 2024-07-24T04:01:03Z Abstract Mosses have adapted to the Antarctic environment and are an ideal medium for studying plant resistance to abiotic stress. Chalcone synthase is the first committed enzyme in the flavonoid metabolic pathway, which plays an indispensable role in plant resistance to adversity. In this study, six genes ( Pn021, PnCHS088, Pn270, PnCHS444, PnCHS768 and Pn847 ) were identified in the Antarctic moss Pohlia nutans Lindberg transcriptome by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Sequence alignment and three-dimensional structure analysis revealed the conserved amino acid residues of the enzymes of the chalcone synthase family, including three catalytic residues (Cys 164 , His 303 and Asn 336 ) and two substrate recognition residues (Phe 215 and Phe 265 ). Phylogenetic analysis indicated that PnCHS088, PnCHS444 and PnCHS768 might be chalcone synthase but that Pn021 is more like stilbenecarboxylate synthase. These genes were located at the transition between fungi and advanced plants in the phylogenetic tree. In addition, real-time PCR analysis revealed that the expression profiles of the six P. nutans genes were influenced by diverse abiotic stresses as well as by abscisic acid and methyl jasmonate. The results presented here contribute to the study of the CHS gene family in polar mosses and further reveal the mechanisms underlying the adaptation of mosses to extreme environments. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Cambridge University Press Antarctic Science 31 1 23 33 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
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English |
description |
Abstract Mosses have adapted to the Antarctic environment and are an ideal medium for studying plant resistance to abiotic stress. Chalcone synthase is the first committed enzyme in the flavonoid metabolic pathway, which plays an indispensable role in plant resistance to adversity. In this study, six genes ( Pn021, PnCHS088, Pn270, PnCHS444, PnCHS768 and Pn847 ) were identified in the Antarctic moss Pohlia nutans Lindberg transcriptome by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Sequence alignment and three-dimensional structure analysis revealed the conserved amino acid residues of the enzymes of the chalcone synthase family, including three catalytic residues (Cys 164 , His 303 and Asn 336 ) and two substrate recognition residues (Phe 215 and Phe 265 ). Phylogenetic analysis indicated that PnCHS088, PnCHS444 and PnCHS768 might be chalcone synthase but that Pn021 is more like stilbenecarboxylate synthase. These genes were located at the transition between fungi and advanced plants in the phylogenetic tree. In addition, real-time PCR analysis revealed that the expression profiles of the six P. nutans genes were influenced by diverse abiotic stresses as well as by abscisic acid and methyl jasmonate. The results presented here contribute to the study of the CHS gene family in polar mosses and further reveal the mechanisms underlying the adaptation of mosses to extreme environments. |
format |
Article in Journal/Newspaper |
author |
Yao, Xinghao Wang, Tailin Wang, Huijuan Liu, Hongwei Liu, Shenghao Zhao, Qingang Chen, Kaoshan Zhang, Pengying |
spellingShingle |
Yao, Xinghao Wang, Tailin Wang, Huijuan Liu, Hongwei Liu, Shenghao Zhao, Qingang Chen, Kaoshan Zhang, Pengying Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans |
author_facet |
Yao, Xinghao Wang, Tailin Wang, Huijuan Liu, Hongwei Liu, Shenghao Zhao, Qingang Chen, Kaoshan Zhang, Pengying |
author_sort |
Yao, Xinghao |
title |
Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans |
title_short |
Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans |
title_full |
Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans |
title_fullStr |
Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans |
title_full_unstemmed |
Identification, characterization and expression analysis of the chalcone synthase family in the Antarctic moss Pohlia nutans |
title_sort |
identification, characterization and expression analysis of the chalcone synthase family in the antarctic moss pohlia nutans |
publisher |
Cambridge University Press (CUP) |
publishDate |
2019 |
url |
http://dx.doi.org/10.1017/s0954102018000470 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102018000470 |
genre |
Antarc* Antarctic Antarctic Science |
genre_facet |
Antarc* Antarctic Antarctic Science |
op_source |
Antarctic Science volume 31, issue 1, page 23-33 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102018000470 |
container_title |
Antarctic Science |
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31 |
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1 |
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23 |
op_container_end_page |
33 |
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1810487356171485184 |