Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective
Abstract The diet of Antarctic salps was elucidated by investigating their gut content using automated ribosomal intergenic spacer analysis (ARISA) and 454-pyrosequencing. Salp samples were collected during the Lazarev Sea Krill Study in the western Weddell Sea (summer 2005–06 and 2007–08, autumn 20...
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crcambridgeupr:10.1017/s0954102014000157 2024-09-15T17:41:32+00:00 Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective Metfies, Katja Nicolaus, Anja Von Harbou, Lena Bathmann, Ulrich Peeken, Ilka 2014 http://dx.doi.org/10.1017/s0954102014000157 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102014000157 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 26, issue 5, page 545-553 ISSN 0954-1020 1365-2079 journal-article 2014 crcambridgeupr https://doi.org/10.1017/s0954102014000157 2024-07-03T04:03:12Z Abstract The diet of Antarctic salps was elucidated by investigating their gut content using automated ribosomal intergenic spacer analysis (ARISA) and 454-pyrosequencing. Salp samples were collected during the Lazarev Sea Krill Study in the western Weddell Sea (summer 2005–06 and 2007–08, autumn 2004 and winter 2006). Two salp species, Salpa thompsoni and Ihlea racovitzai , both occur in the Southern Ocean and can overlap geographically and seasonally. We provide evidence that, despite the non-selective feeding mechanism, the two co-occurring salp species might have different niches within a habitat. ARISA-patterns of 93 gut content samples revealed strong differences between the two salp species, even at the same sampling site. These differences were confirmed by 454-pyrosequencing of the V4-18S rDNA of ten salps. The pyrosequencing data indicate that flagellates, in particular dinophyceae, constitute a high proportion of the sequence reads identified in the gut content of both salp species. However, within the dinophyceae, differences in the read composition were detected between the two salp species. This supports the findings of a previous study where fatty acid signatures indicate a flagellate-based diet, even though microscopic analyses identified diatoms as the dominant component of salp gut contents. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Lazarev Sea Southern Ocean Weddell Sea Cambridge University Press Antarctic Science 26 5 545 553 |
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Open Polar |
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Cambridge University Press |
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English |
description |
Abstract The diet of Antarctic salps was elucidated by investigating their gut content using automated ribosomal intergenic spacer analysis (ARISA) and 454-pyrosequencing. Salp samples were collected during the Lazarev Sea Krill Study in the western Weddell Sea (summer 2005–06 and 2007–08, autumn 2004 and winter 2006). Two salp species, Salpa thompsoni and Ihlea racovitzai , both occur in the Southern Ocean and can overlap geographically and seasonally. We provide evidence that, despite the non-selective feeding mechanism, the two co-occurring salp species might have different niches within a habitat. ARISA-patterns of 93 gut content samples revealed strong differences between the two salp species, even at the same sampling site. These differences were confirmed by 454-pyrosequencing of the V4-18S rDNA of ten salps. The pyrosequencing data indicate that flagellates, in particular dinophyceae, constitute a high proportion of the sequence reads identified in the gut content of both salp species. However, within the dinophyceae, differences in the read composition were detected between the two salp species. This supports the findings of a previous study where fatty acid signatures indicate a flagellate-based diet, even though microscopic analyses identified diatoms as the dominant component of salp gut contents. |
format |
Article in Journal/Newspaper |
author |
Metfies, Katja Nicolaus, Anja Von Harbou, Lena Bathmann, Ulrich Peeken, Ilka |
spellingShingle |
Metfies, Katja Nicolaus, Anja Von Harbou, Lena Bathmann, Ulrich Peeken, Ilka Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective |
author_facet |
Metfies, Katja Nicolaus, Anja Von Harbou, Lena Bathmann, Ulrich Peeken, Ilka |
author_sort |
Metfies, Katja |
title |
Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective |
title_short |
Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective |
title_full |
Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective |
title_fullStr |
Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective |
title_full_unstemmed |
Molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the Lazarev Sea from a different perspective |
title_sort |
molecular analyses of gut contents: elucidating the feeding of co-occurring salps in the lazarev sea from a different perspective |
publisher |
Cambridge University Press (CUP) |
publishDate |
2014 |
url |
http://dx.doi.org/10.1017/s0954102014000157 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102014000157 |
genre |
Antarc* Antarctic Antarctic Science Lazarev Sea Southern Ocean Weddell Sea |
genre_facet |
Antarc* Antarctic Antarctic Science Lazarev Sea Southern Ocean Weddell Sea |
op_source |
Antarctic Science volume 26, issue 5, page 545-553 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102014000157 |
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Antarctic Science |
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26 |
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5 |
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545 |
op_container_end_page |
553 |
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1810487747337519104 |