Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach

Abstract Despite their pivotal role as primary producers, there is little information as to the diversity and physiology of cyanobacteria in the meltwater ecosystems of polar regions. Thirty cyanobacterial mats from Adelaide Island, Antarctica were investigated using 16S rRNA gene pyrosequencing and...

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Published in:Antarctic Science
Main Authors: Kleinteich, J., Hildebrand, F., Wood, S.A., Ciŕs, S., Agha, R., Quesada, A., Pearce, D.A., Convey, P., K̈pper, F.C., Dietrich, D.R.
Format: Article in Journal/Newspaper
Language:English
Published: Cambridge University Press (CUP) 2014
Subjects:
Online Access:http://dx.doi.org/10.1017/s0954102014000145
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102014000145
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spelling crcambridgeupr:10.1017/s0954102014000145 2024-10-13T14:00:53+00:00 Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach Kleinteich, J. Hildebrand, F. Wood, S.A. Ciŕs, S. Agha, R. Quesada, A. Pearce, D.A. Convey, P. K̈pper, F.C. Dietrich, D.R. 2014 http://dx.doi.org/10.1017/s0954102014000145 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102014000145 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 26, issue 5, page 521-532 ISSN 0954-1020 1365-2079 journal-article 2014 crcambridgeupr https://doi.org/10.1017/s0954102014000145 2024-09-18T04:03:34Z Abstract Despite their pivotal role as primary producers, there is little information as to the diversity and physiology of cyanobacteria in the meltwater ecosystems of polar regions. Thirty cyanobacterial mats from Adelaide Island, Antarctica were investigated using 16S rRNA gene pyrosequencing and automated ribosomal intergenic spacer analysis, and screened for cyanobacterial toxins using molecular and chemical approaches. A total of 274 operational taxonomic units (OTUs) were detected. The richness ranged between 8 and 33 cyanobacterial OTUs per sample, reflecting a high mat diversity. Leptolyngbya and Phormidium ( c . 55% and 37% of the OTUs per mat) were dominant. Cyanobacterial community composition was similar between mats, particularly those obtained from closely adjacent locations. The cyanotoxin microcystin was detected in 26 of 27 mats (10–300 ng g -1 organic mass), while cylindrospermopsin, detected for the first time in Antarctica, was present in 21 of 30 mats (2–156 ng g -1 organic mass). The latter was confirmed via liquid chromatography-mass spectrometry and by the presence of the cyrAB and cyrJ genes. This study demonstrates the usefulness of pyrosequencing for characterizing diverse cyanobacterial communities, and confirms that cyanobacteria from extreme environments produce a similar range of cyanotoxins as their temperate counterparts. Article in Journal/Newspaper Adelaide Island Antarc* Antarctic Antarctic Peninsula Antarctic Science Antarctica Cambridge University Press Antarctic The Antarctic Antarctic Peninsula Adelaide Island ENVELOPE(-68.914,-68.914,-67.762,-67.762) Antarctic Science 26 5 521 532
institution Open Polar
collection Cambridge University Press
op_collection_id crcambridgeupr
language English
description Abstract Despite their pivotal role as primary producers, there is little information as to the diversity and physiology of cyanobacteria in the meltwater ecosystems of polar regions. Thirty cyanobacterial mats from Adelaide Island, Antarctica were investigated using 16S rRNA gene pyrosequencing and automated ribosomal intergenic spacer analysis, and screened for cyanobacterial toxins using molecular and chemical approaches. A total of 274 operational taxonomic units (OTUs) were detected. The richness ranged between 8 and 33 cyanobacterial OTUs per sample, reflecting a high mat diversity. Leptolyngbya and Phormidium ( c . 55% and 37% of the OTUs per mat) were dominant. Cyanobacterial community composition was similar between mats, particularly those obtained from closely adjacent locations. The cyanotoxin microcystin was detected in 26 of 27 mats (10–300 ng g -1 organic mass), while cylindrospermopsin, detected for the first time in Antarctica, was present in 21 of 30 mats (2–156 ng g -1 organic mass). The latter was confirmed via liquid chromatography-mass spectrometry and by the presence of the cyrAB and cyrJ genes. This study demonstrates the usefulness of pyrosequencing for characterizing diverse cyanobacterial communities, and confirms that cyanobacteria from extreme environments produce a similar range of cyanotoxins as their temperate counterparts.
format Article in Journal/Newspaper
author Kleinteich, J.
Hildebrand, F.
Wood, S.A.
Ciŕs, S.
Agha, R.
Quesada, A.
Pearce, D.A.
Convey, P.
K̈pper, F.C.
Dietrich, D.R.
spellingShingle Kleinteich, J.
Hildebrand, F.
Wood, S.A.
Ciŕs, S.
Agha, R.
Quesada, A.
Pearce, D.A.
Convey, P.
K̈pper, F.C.
Dietrich, D.R.
Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach
author_facet Kleinteich, J.
Hildebrand, F.
Wood, S.A.
Ciŕs, S.
Agha, R.
Quesada, A.
Pearce, D.A.
Convey, P.
K̈pper, F.C.
Dietrich, D.R.
author_sort Kleinteich, J.
title Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach
title_short Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach
title_full Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach
title_fullStr Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach
title_full_unstemmed Diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the Antarctic Peninsula: a pyrosequencing approach
title_sort diversity of toxin and non-toxin containing cyanobacterial mats of meltwater ponds on the antarctic peninsula: a pyrosequencing approach
publisher Cambridge University Press (CUP)
publishDate 2014
url http://dx.doi.org/10.1017/s0954102014000145
https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102014000145
long_lat ENVELOPE(-68.914,-68.914,-67.762,-67.762)
geographic Antarctic
The Antarctic
Antarctic Peninsula
Adelaide Island
geographic_facet Antarctic
The Antarctic
Antarctic Peninsula
Adelaide Island
genre Adelaide Island
Antarc*
Antarctic
Antarctic Peninsula
Antarctic Science
Antarctica
genre_facet Adelaide Island
Antarc*
Antarctic
Antarctic Peninsula
Antarctic Science
Antarctica
op_source Antarctic Science
volume 26, issue 5, page 521-532
ISSN 0954-1020 1365-2079
op_rights https://www.cambridge.org/core/terms
op_doi https://doi.org/10.1017/s0954102014000145
container_title Antarctic Science
container_volume 26
container_issue 5
container_start_page 521
op_container_end_page 532
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