Reference genes validation for qPCR normalization in Deschampsia antarcticaduring abiotic stresses
Abstract Quantitative real time PCR is the most sensitive and widely used method for the analysis of gene expression. The choice of one or several reference genes is very important for a normalization process, which should not fluctuate under stress conditions, to reduce error rate and bias during e...
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crcambridgeupr:10.1017/s0954102010000428 2024-09-09T19:06:53+00:00 Reference genes validation for qPCR normalization in Deschampsia antarcticaduring abiotic stresses Lee, Hyoungseok Kim, Ji Hyun Park, Mira Kim, Il-Chan Yim, Joung Han Lee, Hong Kum 2010 http://dx.doi.org/10.1017/s0954102010000428 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102010000428 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 22, issue 5, page 477-484 ISSN 0954-1020 1365-2079 journal-article 2010 crcambridgeupr https://doi.org/10.1017/s0954102010000428 2024-06-19T04:04:13Z Abstract Quantitative real time PCR is the most sensitive and widely used method for the analysis of gene expression. The choice of one or several reference genes is very important for a normalization process, which should not fluctuate under stress conditions, to reduce error rate and bias during experimental procedure. In the present study, the expression stability of nine reference genes (two actins, two tubulins, two elongation factor 1α, two ubiquitins, and cyclophilin) during abiotic stresses such as cold, salt, and PEG treatments, was evaluated on Deschampsia antarctica plants using geNorm software. Results from various experimental conditions indicated that cyclophilin and elongation factor 1α were the most stable genes in the leaf and the root, respectively. The expression of the other reference genes varied under stress. The relative quantification of the TACR7 gene varied according to the kind and the number of reference genes used, suggesting the importance of considering the implications of a combination of reference genes under different stress conditions and in different tissues. Article in Journal/Newspaper Antarc* Antarctic Science Antarctica Cambridge University Press Antarctic Science 22 5 477 484 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
Abstract Quantitative real time PCR is the most sensitive and widely used method for the analysis of gene expression. The choice of one or several reference genes is very important for a normalization process, which should not fluctuate under stress conditions, to reduce error rate and bias during experimental procedure. In the present study, the expression stability of nine reference genes (two actins, two tubulins, two elongation factor 1α, two ubiquitins, and cyclophilin) during abiotic stresses such as cold, salt, and PEG treatments, was evaluated on Deschampsia antarctica plants using geNorm software. Results from various experimental conditions indicated that cyclophilin and elongation factor 1α were the most stable genes in the leaf and the root, respectively. The expression of the other reference genes varied under stress. The relative quantification of the TACR7 gene varied according to the kind and the number of reference genes used, suggesting the importance of considering the implications of a combination of reference genes under different stress conditions and in different tissues. |
format |
Article in Journal/Newspaper |
author |
Lee, Hyoungseok Kim, Ji Hyun Park, Mira Kim, Il-Chan Yim, Joung Han Lee, Hong Kum |
spellingShingle |
Lee, Hyoungseok Kim, Ji Hyun Park, Mira Kim, Il-Chan Yim, Joung Han Lee, Hong Kum Reference genes validation for qPCR normalization in Deschampsia antarcticaduring abiotic stresses |
author_facet |
Lee, Hyoungseok Kim, Ji Hyun Park, Mira Kim, Il-Chan Yim, Joung Han Lee, Hong Kum |
author_sort |
Lee, Hyoungseok |
title |
Reference genes validation for qPCR normalization in Deschampsia antarcticaduring abiotic stresses |
title_short |
Reference genes validation for qPCR normalization in Deschampsia antarcticaduring abiotic stresses |
title_full |
Reference genes validation for qPCR normalization in Deschampsia antarcticaduring abiotic stresses |
title_fullStr |
Reference genes validation for qPCR normalization in Deschampsia antarcticaduring abiotic stresses |
title_full_unstemmed |
Reference genes validation for qPCR normalization in Deschampsia antarcticaduring abiotic stresses |
title_sort |
reference genes validation for qpcr normalization in deschampsia antarcticaduring abiotic stresses |
publisher |
Cambridge University Press (CUP) |
publishDate |
2010 |
url |
http://dx.doi.org/10.1017/s0954102010000428 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102010000428 |
genre |
Antarc* Antarctic Science Antarctica |
genre_facet |
Antarc* Antarctic Science Antarctica |
op_source |
Antarctic Science volume 22, issue 5, page 477-484 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102010000428 |
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Antarctic Science |
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22 |
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5 |
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477 |
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484 |
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1809820948180762624 |