Viable microbes in ice: application of molecular assays to McMurdo Dry Valley lake ice communities

• Published in: Antarctic Science
• Main Authors: Dieser, Markus, Nocker, Andreas, Priscu, John C., Foreman, Christine M.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Cambridge University Press (CUP) 2010
• Subjects: Bonney; Fryxell; Hoare; Lake Bonney; Lake Fryxell; Lake Hoare; Antarc*; Antarctic Science; Antarctica
• Online Access: http://dx.doi.org/10.1017/s0954102010000404; https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102010000404

Abstract The permanent ice covers of the McMurdo Dry Valley lakes, Antarctica, are colonized by a diverse microbial assemblage. We collected ice cores from Lakes Fryxell, Hoare and Bonney. Propidium monoazide (PMA) was used in combination with quantitative PCR (qPCR) and denaturing gradient gel elec...