Viable microbes in ice: application of molecular assays to McMurdo Dry Valley lake ice communities
Abstract The permanent ice covers of the McMurdo Dry Valley lakes, Antarctica, are colonized by a diverse microbial assemblage. We collected ice cores from Lakes Fryxell, Hoare and Bonney. Propidium monoazide (PMA) was used in combination with quantitative PCR (qPCR) and denaturing gradient gel elec...
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Online Access: | http://dx.doi.org/10.1017/s0954102010000404 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102010000404 |
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crcambridgeupr:10.1017/s0954102010000404 2024-06-23T07:47:28+00:00 Viable microbes in ice: application of molecular assays to McMurdo Dry Valley lake ice communities Dieser, Markus Nocker, Andreas Priscu, John C. Foreman, Christine M. 2010 http://dx.doi.org/10.1017/s0954102010000404 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102010000404 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 22, issue 5, page 470-476 ISSN 0954-1020 1365-2079 journal-article 2010 crcambridgeupr https://doi.org/10.1017/s0954102010000404 2024-06-05T04:01:17Z Abstract The permanent ice covers of the McMurdo Dry Valley lakes, Antarctica, are colonized by a diverse microbial assemblage. We collected ice cores from Lakes Fryxell, Hoare and Bonney. Propidium monoazide (PMA) was used in combination with quantitative PCR (qPCR) and denaturing gradient gel electrophoresis (DGGE) to examine membrane integrity of prokaryotes in these extreme environments. PMA selectively penetrates cells with compromised membranes and modifies their DNA resulting in the suppression of PCR amplification. Our results based on analysis of 16S rRNA genes demonstrate that despite the hostile conditions of the Dry Valleys, the permanent ice covers of the lakes support a ‘potentially viable’ microbial community. The level of membrane integrity, as well as diversity, was higher in samples where sediment was entrapped in the ice cover. Pronounced differences in the fraction of cells with intact and compromised cell membranes were found for Lake Fryxell and east lobe of Lake Bonney, both expressed in differences in DGGE banding patterns and qPCR signal reductions. Limitations in the ability to distinguish between intact or compromised cells occurred in samples from Lake Hoare and west lobe of Lake Bonney due to low DNA template concentrations recovered from the samples. Article in Journal/Newspaper Antarc* Antarctic Science Antarctica Cambridge University Press Bonney ENVELOPE(162.417,162.417,-77.717,-77.717) Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) Hoare ENVELOPE(162.850,162.850,-77.633,-77.633) Lake Bonney ENVELOPE(-25.588,-25.588,-80.361,-80.361) Lake Fryxell ENVELOPE(163.183,163.183,-77.617,-77.617) Lake Hoare ENVELOPE(162.850,162.850,-77.633,-77.633) Antarctic Science 22 5 470 476 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
description |
Abstract The permanent ice covers of the McMurdo Dry Valley lakes, Antarctica, are colonized by a diverse microbial assemblage. We collected ice cores from Lakes Fryxell, Hoare and Bonney. Propidium monoazide (PMA) was used in combination with quantitative PCR (qPCR) and denaturing gradient gel electrophoresis (DGGE) to examine membrane integrity of prokaryotes in these extreme environments. PMA selectively penetrates cells with compromised membranes and modifies their DNA resulting in the suppression of PCR amplification. Our results based on analysis of 16S rRNA genes demonstrate that despite the hostile conditions of the Dry Valleys, the permanent ice covers of the lakes support a ‘potentially viable’ microbial community. The level of membrane integrity, as well as diversity, was higher in samples where sediment was entrapped in the ice cover. Pronounced differences in the fraction of cells with intact and compromised cell membranes were found for Lake Fryxell and east lobe of Lake Bonney, both expressed in differences in DGGE banding patterns and qPCR signal reductions. Limitations in the ability to distinguish between intact or compromised cells occurred in samples from Lake Hoare and west lobe of Lake Bonney due to low DNA template concentrations recovered from the samples. |
format |
Article in Journal/Newspaper |
author |
Dieser, Markus Nocker, Andreas Priscu, John C. Foreman, Christine M. |
spellingShingle |
Dieser, Markus Nocker, Andreas Priscu, John C. Foreman, Christine M. Viable microbes in ice: application of molecular assays to McMurdo Dry Valley lake ice communities |
author_facet |
Dieser, Markus Nocker, Andreas Priscu, John C. Foreman, Christine M. |
author_sort |
Dieser, Markus |
title |
Viable microbes in ice: application of molecular assays to McMurdo Dry Valley lake ice communities |
title_short |
Viable microbes in ice: application of molecular assays to McMurdo Dry Valley lake ice communities |
title_full |
Viable microbes in ice: application of molecular assays to McMurdo Dry Valley lake ice communities |
title_fullStr |
Viable microbes in ice: application of molecular assays to McMurdo Dry Valley lake ice communities |
title_full_unstemmed |
Viable microbes in ice: application of molecular assays to McMurdo Dry Valley lake ice communities |
title_sort |
viable microbes in ice: application of molecular assays to mcmurdo dry valley lake ice communities |
publisher |
Cambridge University Press (CUP) |
publishDate |
2010 |
url |
http://dx.doi.org/10.1017/s0954102010000404 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102010000404 |
long_lat |
ENVELOPE(162.417,162.417,-77.717,-77.717) ENVELOPE(163.183,163.183,-77.617,-77.617) ENVELOPE(162.850,162.850,-77.633,-77.633) ENVELOPE(-25.588,-25.588,-80.361,-80.361) ENVELOPE(163.183,163.183,-77.617,-77.617) ENVELOPE(162.850,162.850,-77.633,-77.633) |
geographic |
Bonney Fryxell Hoare Lake Bonney Lake Fryxell Lake Hoare |
geographic_facet |
Bonney Fryxell Hoare Lake Bonney Lake Fryxell Lake Hoare |
genre |
Antarc* Antarctic Science Antarctica |
genre_facet |
Antarc* Antarctic Science Antarctica |
op_source |
Antarctic Science volume 22, issue 5, page 470-476 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102010000404 |
container_title |
Antarctic Science |
container_volume |
22 |
container_issue |
5 |
container_start_page |
470 |
op_container_end_page |
476 |
_version_ |
1802651575092510720 |