Small-scale studies towards a rational use of bioaugmentation in an Antarctic hydrocarbon-contaminated soil
Abstract Bioaugmentation is a controversial strategy. In this work, the effect of the inoculum size and the absence of natural microflora on the efficiency of hydrocarbon removal were studied. Two levels of inoculum (10 6 and 10 9 CFU g -1 ) were applied to soil microcosms containing sterile (S6 and...
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Online Access: | http://dx.doi.org/10.1017/s0954102010000295 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102010000295 |
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crcambridgeupr:10.1017/s0954102010000295 2024-09-09T19:05:49+00:00 Small-scale studies towards a rational use of bioaugmentation in an Antarctic hydrocarbon-contaminated soil Ruberto, Lucas Vazquez, Susana C. Dias, Romina L. Hernández, Edgardo A. Coria, Silvia H. Levin, Gustavo Lo Balbo, Alfredo Mac Cormack, Walter P. 2010 http://dx.doi.org/10.1017/s0954102010000295 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102010000295 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 22, issue 5, page 463-469 ISSN 0954-1020 1365-2079 journal-article 2010 crcambridgeupr https://doi.org/10.1017/s0954102010000295 2024-07-17T04:04:06Z Abstract Bioaugmentation is a controversial strategy. In this work, the effect of the inoculum size and the absence of natural microflora on the efficiency of hydrocarbon removal were studied. Two levels of inoculum (10 6 and 10 9 CFU g -1 ) were applied to soil microcosms containing sterile (S6 and S9) and non-sterile (NS6 and NS9) oil contaminated Antarctic soil. Community controls (CC) and biostimulated autochthonous microflora (BAM) were also included. Total heterotrophic aerobic (THAB) and hydrocarbon degrading (HDB) bacteria as well as total petroleum hydrocarbons (TPH) were analysed. At day 0, THAB and HDB counts (CFU g -1 ) showed no differences among CC, BAM and NS6 but significantly higher values were observed in NS9 and S9. At day 60, three different levels of HDB were observed. The lower level was represented by CC (10 6 CFU g -1 ), a second group (5 x 10 7 CFU g -1 ) was represented by BAM, NS6, NS9 and S6, and the third level was constituted by S9 (1 x 10 9 CFU g -1 ). TPH values at day 60 decreased significantly in all systems excluding the controls. NS6, NS9, S6 and S9 were not different from those corresponding to BAM. Results suggest that the bioaugmentation of a chronically diesel fuel-contaminated Antarctic soil is unlikely to be profitable or beneficial. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Cambridge University Press Antarctic Antarctic Science 22 5 463 469 |
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Open Polar |
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Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
description |
Abstract Bioaugmentation is a controversial strategy. In this work, the effect of the inoculum size and the absence of natural microflora on the efficiency of hydrocarbon removal were studied. Two levels of inoculum (10 6 and 10 9 CFU g -1 ) were applied to soil microcosms containing sterile (S6 and S9) and non-sterile (NS6 and NS9) oil contaminated Antarctic soil. Community controls (CC) and biostimulated autochthonous microflora (BAM) were also included. Total heterotrophic aerobic (THAB) and hydrocarbon degrading (HDB) bacteria as well as total petroleum hydrocarbons (TPH) were analysed. At day 0, THAB and HDB counts (CFU g -1 ) showed no differences among CC, BAM and NS6 but significantly higher values were observed in NS9 and S9. At day 60, three different levels of HDB were observed. The lower level was represented by CC (10 6 CFU g -1 ), a second group (5 x 10 7 CFU g -1 ) was represented by BAM, NS6, NS9 and S6, and the third level was constituted by S9 (1 x 10 9 CFU g -1 ). TPH values at day 60 decreased significantly in all systems excluding the controls. NS6, NS9, S6 and S9 were not different from those corresponding to BAM. Results suggest that the bioaugmentation of a chronically diesel fuel-contaminated Antarctic soil is unlikely to be profitable or beneficial. |
format |
Article in Journal/Newspaper |
author |
Ruberto, Lucas Vazquez, Susana C. Dias, Romina L. Hernández, Edgardo A. Coria, Silvia H. Levin, Gustavo Lo Balbo, Alfredo Mac Cormack, Walter P. |
spellingShingle |
Ruberto, Lucas Vazquez, Susana C. Dias, Romina L. Hernández, Edgardo A. Coria, Silvia H. Levin, Gustavo Lo Balbo, Alfredo Mac Cormack, Walter P. Small-scale studies towards a rational use of bioaugmentation in an Antarctic hydrocarbon-contaminated soil |
author_facet |
Ruberto, Lucas Vazquez, Susana C. Dias, Romina L. Hernández, Edgardo A. Coria, Silvia H. Levin, Gustavo Lo Balbo, Alfredo Mac Cormack, Walter P. |
author_sort |
Ruberto, Lucas |
title |
Small-scale studies towards a rational use of bioaugmentation in an Antarctic hydrocarbon-contaminated soil |
title_short |
Small-scale studies towards a rational use of bioaugmentation in an Antarctic hydrocarbon-contaminated soil |
title_full |
Small-scale studies towards a rational use of bioaugmentation in an Antarctic hydrocarbon-contaminated soil |
title_fullStr |
Small-scale studies towards a rational use of bioaugmentation in an Antarctic hydrocarbon-contaminated soil |
title_full_unstemmed |
Small-scale studies towards a rational use of bioaugmentation in an Antarctic hydrocarbon-contaminated soil |
title_sort |
small-scale studies towards a rational use of bioaugmentation in an antarctic hydrocarbon-contaminated soil |
publisher |
Cambridge University Press (CUP) |
publishDate |
2010 |
url |
http://dx.doi.org/10.1017/s0954102010000295 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102010000295 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Science |
genre_facet |
Antarc* Antarctic Antarctic Science |
op_source |
Antarctic Science volume 22, issue 5, page 463-469 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102010000295 |
container_title |
Antarctic Science |
container_volume |
22 |
container_issue |
5 |
container_start_page |
463 |
op_container_end_page |
469 |
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1809819815345389568 |