Water masses’ bacterial community structure and microbial activities in the Ross Sea, Antarctica
Abstract During the summer 2005/06, an oceanographic cruise was carried out in the Ross Sea, from Cape Adare, through the Terra Nova Bay polynya to the eastern edge of the Ross Ice Shelf. We analysed microbial activities (prokaryotic carbon production, protease, phosphatase, beta-glucosidase and lip...
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crcambridgeupr:10.1017/s0954102010000192 2024-09-15T17:40:37+00:00 Water masses’ bacterial community structure and microbial activities in the Ross Sea, Antarctica Celussi, Mauro Bergamasco, Andrea Cataletto, Bruno Umani, Serena Fonda Del Negro, Paola 2010 http://dx.doi.org/10.1017/s0954102010000192 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102010000192 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 22, issue 4, page 361-370 ISSN 0954-1020 1365-2079 journal-article 2010 crcambridgeupr https://doi.org/10.1017/s0954102010000192 2024-08-21T04:04:06Z Abstract During the summer 2005/06, an oceanographic cruise was carried out in the Ross Sea, from Cape Adare, through the Terra Nova Bay polynya to the eastern edge of the Ross Ice Shelf. We analysed microbial activities (prokaryotic carbon production, protease, phosphatase, beta-glucosidase and lipase activity) and bacterial community structure (using Denaturing Gradient Gel Electrophoresis - DGGE) in order to establish if differences in bacterioplankton assemblages and their metabolic requirements occur within the five Ross Sea water masses: Antarctic Surface Waters (AASW), High Salinity Shelf Water (HSSW), Ice Shelf Water (ISW), Antarctic Bottom Water (AABW), Circumpolar Deep Water (CDW). Differences in activities were found between the highly active AASW and all the other water bodies. A Principal Component Analysis highlighted two main gradients: in the Cape Adare area (AASWn, CDW and AABW) higher phosphatase, lipase and glycolytic activities, increasing towards the surface, were identified, whereas in the southern sector of the basin [AASWs and (m)HSSW] higher leucine uptake and polypeptide degradation characterized the second gradient. DGGE fingerprinting showed for the first time that different water masses harboured diverse bacterial communities, highlighting the high specificity of deep water assemblages. Alpha - and Gammaproteobacteria represented the main phylogenetic groupings in all samples and no substantial difference in the phylogenetic composition of assemblages was found between different water masses. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Antarctica Ice Shelf Ross Ice Shelf Ross Sea Cambridge University Press Antarctic Science 22 4 361 370 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
Abstract During the summer 2005/06, an oceanographic cruise was carried out in the Ross Sea, from Cape Adare, through the Terra Nova Bay polynya to the eastern edge of the Ross Ice Shelf. We analysed microbial activities (prokaryotic carbon production, protease, phosphatase, beta-glucosidase and lipase activity) and bacterial community structure (using Denaturing Gradient Gel Electrophoresis - DGGE) in order to establish if differences in bacterioplankton assemblages and their metabolic requirements occur within the five Ross Sea water masses: Antarctic Surface Waters (AASW), High Salinity Shelf Water (HSSW), Ice Shelf Water (ISW), Antarctic Bottom Water (AABW), Circumpolar Deep Water (CDW). Differences in activities were found between the highly active AASW and all the other water bodies. A Principal Component Analysis highlighted two main gradients: in the Cape Adare area (AASWn, CDW and AABW) higher phosphatase, lipase and glycolytic activities, increasing towards the surface, were identified, whereas in the southern sector of the basin [AASWs and (m)HSSW] higher leucine uptake and polypeptide degradation characterized the second gradient. DGGE fingerprinting showed for the first time that different water masses harboured diverse bacterial communities, highlighting the high specificity of deep water assemblages. Alpha - and Gammaproteobacteria represented the main phylogenetic groupings in all samples and no substantial difference in the phylogenetic composition of assemblages was found between different water masses. |
format |
Article in Journal/Newspaper |
author |
Celussi, Mauro Bergamasco, Andrea Cataletto, Bruno Umani, Serena Fonda Del Negro, Paola |
spellingShingle |
Celussi, Mauro Bergamasco, Andrea Cataletto, Bruno Umani, Serena Fonda Del Negro, Paola Water masses’ bacterial community structure and microbial activities in the Ross Sea, Antarctica |
author_facet |
Celussi, Mauro Bergamasco, Andrea Cataletto, Bruno Umani, Serena Fonda Del Negro, Paola |
author_sort |
Celussi, Mauro |
title |
Water masses’ bacterial community structure and microbial activities in the Ross Sea, Antarctica |
title_short |
Water masses’ bacterial community structure and microbial activities in the Ross Sea, Antarctica |
title_full |
Water masses’ bacterial community structure and microbial activities in the Ross Sea, Antarctica |
title_fullStr |
Water masses’ bacterial community structure and microbial activities in the Ross Sea, Antarctica |
title_full_unstemmed |
Water masses’ bacterial community structure and microbial activities in the Ross Sea, Antarctica |
title_sort |
water masses’ bacterial community structure and microbial activities in the ross sea, antarctica |
publisher |
Cambridge University Press (CUP) |
publishDate |
2010 |
url |
http://dx.doi.org/10.1017/s0954102010000192 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102010000192 |
genre |
Antarc* Antarctic Antarctic Science Antarctica Ice Shelf Ross Ice Shelf Ross Sea |
genre_facet |
Antarc* Antarctic Antarctic Science Antarctica Ice Shelf Ross Ice Shelf Ross Sea |
op_source |
Antarctic Science volume 22, issue 4, page 361-370 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102010000192 |
container_title |
Antarctic Science |
container_volume |
22 |
container_issue |
4 |
container_start_page |
361 |
op_container_end_page |
370 |
_version_ |
1810486644893024256 |