Biodiversity of the bacterioplankton in the surface waters around Southern Thule in the Southern Ocean
Abstract Seven independent clone libraries were constructed to study the biodiversity of the bacterioplankton in the surface waters around Southern Thule, South Sandwich Islands, in order to identify the species present, to determine the sample effort required to estimate the total diversity, and to...
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2008
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Online Access: | http://dx.doi.org/10.1017/s095410200800117x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S095410200800117X |
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crcambridgeupr:10.1017/s095410200800117x 2024-03-03T08:38:57+00:00 Biodiversity of the bacterioplankton in the surface waters around Southern Thule in the Southern Ocean Pearce, David A. 2008 http://dx.doi.org/10.1017/s095410200800117x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S095410200800117X en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 20, issue 3, page 291-300 ISSN 0954-1020 1365-2079 Geology Ecology, Evolution, Behavior and Systematics Oceanography journal-article 2008 crcambridgeupr https://doi.org/10.1017/s095410200800117x 2024-02-08T08:44:23Z Abstract Seven independent clone libraries were constructed to study the biodiversity of the bacterioplankton in the surface waters around Southern Thule, South Sandwich Islands, in order to identify the species present, to determine the sample effort required to estimate the total diversity, and to determine whether the surface waters around Southern Thule represented a highly specialized local anomaly or a subset of the marine meta-community. In total, 672 clones generated 629 useable sequences. These 629 clones matched 278 different sequences deposited in the 16S rDNA sequence databases. The majority of the clones were related to marine microorganisms, many of which had been previously detected in permanently cold Arctic and Antarctic marine environments. Each clone library generated an average of 35.8 new sequence matches. 346 clones covered two-thirds of the total estimated diversity, while 438 clones covered three-quarters of the total estimated diversity. Above this number, the coverage tended to stabilize and a relatively large number of additional clones were required to improve coverage significantly, increasing at the rate of about one new sequence match per 100 new clones. Comparing the different clone libraries, eight matches occurred in each of the seven libraries, whilst fifty-five occurred in only one, suggesting that there might be a relatively small number of common dominant ubiquitous species, with a much larger underlying diversity or ‘seed bank’ from which this dominant diversity is drawn. This study suggests that the dominant bacterioplankton in the surface waters around Southern Thule represent a subset of the marine meta-community, whilst sub-dominant diversity appears to be a highly specialized local anomaly. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Arctic South Sandwich Islands Southern Ocean Southern Thule Cambridge University Press Arctic Antarctic Southern Ocean Sandwich Islands South Sandwich Islands Southern Thule ENVELOPE(-27.200,-27.200,-59.433,-59.433) Antarctic Science 20 3 291 300 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
topic |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
spellingShingle |
Geology Ecology, Evolution, Behavior and Systematics Oceanography Pearce, David A. Biodiversity of the bacterioplankton in the surface waters around Southern Thule in the Southern Ocean |
topic_facet |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
description |
Abstract Seven independent clone libraries were constructed to study the biodiversity of the bacterioplankton in the surface waters around Southern Thule, South Sandwich Islands, in order to identify the species present, to determine the sample effort required to estimate the total diversity, and to determine whether the surface waters around Southern Thule represented a highly specialized local anomaly or a subset of the marine meta-community. In total, 672 clones generated 629 useable sequences. These 629 clones matched 278 different sequences deposited in the 16S rDNA sequence databases. The majority of the clones were related to marine microorganisms, many of which had been previously detected in permanently cold Arctic and Antarctic marine environments. Each clone library generated an average of 35.8 new sequence matches. 346 clones covered two-thirds of the total estimated diversity, while 438 clones covered three-quarters of the total estimated diversity. Above this number, the coverage tended to stabilize and a relatively large number of additional clones were required to improve coverage significantly, increasing at the rate of about one new sequence match per 100 new clones. Comparing the different clone libraries, eight matches occurred in each of the seven libraries, whilst fifty-five occurred in only one, suggesting that there might be a relatively small number of common dominant ubiquitous species, with a much larger underlying diversity or ‘seed bank’ from which this dominant diversity is drawn. This study suggests that the dominant bacterioplankton in the surface waters around Southern Thule represent a subset of the marine meta-community, whilst sub-dominant diversity appears to be a highly specialized local anomaly. |
format |
Article in Journal/Newspaper |
author |
Pearce, David A. |
author_facet |
Pearce, David A. |
author_sort |
Pearce, David A. |
title |
Biodiversity of the bacterioplankton in the surface waters around Southern Thule in the Southern Ocean |
title_short |
Biodiversity of the bacterioplankton in the surface waters around Southern Thule in the Southern Ocean |
title_full |
Biodiversity of the bacterioplankton in the surface waters around Southern Thule in the Southern Ocean |
title_fullStr |
Biodiversity of the bacterioplankton in the surface waters around Southern Thule in the Southern Ocean |
title_full_unstemmed |
Biodiversity of the bacterioplankton in the surface waters around Southern Thule in the Southern Ocean |
title_sort |
biodiversity of the bacterioplankton in the surface waters around southern thule in the southern ocean |
publisher |
Cambridge University Press (CUP) |
publishDate |
2008 |
url |
http://dx.doi.org/10.1017/s095410200800117x https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S095410200800117X |
long_lat |
ENVELOPE(-27.200,-27.200,-59.433,-59.433) |
geographic |
Arctic Antarctic Southern Ocean Sandwich Islands South Sandwich Islands Southern Thule |
geographic_facet |
Arctic Antarctic Southern Ocean Sandwich Islands South Sandwich Islands Southern Thule |
genre |
Antarc* Antarctic Antarctic Science Arctic South Sandwich Islands Southern Ocean Southern Thule |
genre_facet |
Antarc* Antarctic Antarctic Science Arctic South Sandwich Islands Southern Ocean Southern Thule |
op_source |
Antarctic Science volume 20, issue 3, page 291-300 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s095410200800117x |
container_title |
Antarctic Science |
container_volume |
20 |
container_issue |
3 |
container_start_page |
291 |
op_container_end_page |
300 |
_version_ |
1792494361424953344 |