Adaptive evolution of the heat-shock response in the Antarctic psychrophilic ciliate, Euplotes focardii : hints from a comparative determination of the hsp70 gene structure
Abstract The Antarctic psychrophilic ciliate Euplotes focardii manifests a dramatic reduction in the activation of its hsp70 gene in response to a heat-shock, while oxidative and chemical stresses activate the transcription of this gene to appreciable extents. To investigate the genetic causes of th...
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crcambridgeupr:10.1017/s0954102007000314 2024-03-03T08:37:56+00:00 Adaptive evolution of the heat-shock response in the Antarctic psychrophilic ciliate, Euplotes focardii : hints from a comparative determination of the hsp70 gene structure La Terza, Antonietta Passini, Valerio Barchetta, Sabrina Luporini, Pierangelo 2007 http://dx.doi.org/10.1017/s0954102007000314 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102007000314 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 19, issue 2, page 239-244 ISSN 0954-1020 1365-2079 Geology Ecology, Evolution, Behavior and Systematics Oceanography journal-article 2007 crcambridgeupr https://doi.org/10.1017/s0954102007000314 2024-02-08T08:31:33Z Abstract The Antarctic psychrophilic ciliate Euplotes focardii manifests a dramatic reduction in the activation of its hsp70 gene in response to a heat-shock, while oxidative and chemical stresses activate the transcription of this gene to appreciable extents. To investigate the genetic causes of this eccentric behaviour of E. focardii in the hsp70 gene transcription activation, we carried out a comparative structural analysis of this gene between E. focardii and another Antarctic Euplotes , E. nobilii , which manifests a psychrotrophic behaviour and an inducible thermal response. No substantial difference was detected in the organization of the hsp70 5' promoter region, both species bearing canonical regulatory cis -acting elements deputed to bind transcriptional trans -activating factors. Adenine-rich elements favouring mRNA degradation were instead detected in the hsp70 3' regulatory region of E. nobilii , but not in that of E. focardii . These observations lend further support to the hypothesis that the causes of the Euplotes focardii unresponsiveness to thermal stress resides in some structural, or functional modifications of transcriptional trans -activating factors. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Cambridge University Press Antarctic The Antarctic Antarctic Science 19 2 239 244 |
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Open Polar |
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Cambridge University Press |
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crcambridgeupr |
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English |
topic |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
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Geology Ecology, Evolution, Behavior and Systematics Oceanography La Terza, Antonietta Passini, Valerio Barchetta, Sabrina Luporini, Pierangelo Adaptive evolution of the heat-shock response in the Antarctic psychrophilic ciliate, Euplotes focardii : hints from a comparative determination of the hsp70 gene structure |
topic_facet |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
description |
Abstract The Antarctic psychrophilic ciliate Euplotes focardii manifests a dramatic reduction in the activation of its hsp70 gene in response to a heat-shock, while oxidative and chemical stresses activate the transcription of this gene to appreciable extents. To investigate the genetic causes of this eccentric behaviour of E. focardii in the hsp70 gene transcription activation, we carried out a comparative structural analysis of this gene between E. focardii and another Antarctic Euplotes , E. nobilii , which manifests a psychrotrophic behaviour and an inducible thermal response. No substantial difference was detected in the organization of the hsp70 5' promoter region, both species bearing canonical regulatory cis -acting elements deputed to bind transcriptional trans -activating factors. Adenine-rich elements favouring mRNA degradation were instead detected in the hsp70 3' regulatory region of E. nobilii , but not in that of E. focardii . These observations lend further support to the hypothesis that the causes of the Euplotes focardii unresponsiveness to thermal stress resides in some structural, or functional modifications of transcriptional trans -activating factors. |
format |
Article in Journal/Newspaper |
author |
La Terza, Antonietta Passini, Valerio Barchetta, Sabrina Luporini, Pierangelo |
author_facet |
La Terza, Antonietta Passini, Valerio Barchetta, Sabrina Luporini, Pierangelo |
author_sort |
La Terza, Antonietta |
title |
Adaptive evolution of the heat-shock response in the Antarctic psychrophilic ciliate, Euplotes focardii : hints from a comparative determination of the hsp70 gene structure |
title_short |
Adaptive evolution of the heat-shock response in the Antarctic psychrophilic ciliate, Euplotes focardii : hints from a comparative determination of the hsp70 gene structure |
title_full |
Adaptive evolution of the heat-shock response in the Antarctic psychrophilic ciliate, Euplotes focardii : hints from a comparative determination of the hsp70 gene structure |
title_fullStr |
Adaptive evolution of the heat-shock response in the Antarctic psychrophilic ciliate, Euplotes focardii : hints from a comparative determination of the hsp70 gene structure |
title_full_unstemmed |
Adaptive evolution of the heat-shock response in the Antarctic psychrophilic ciliate, Euplotes focardii : hints from a comparative determination of the hsp70 gene structure |
title_sort |
adaptive evolution of the heat-shock response in the antarctic psychrophilic ciliate, euplotes focardii : hints from a comparative determination of the hsp70 gene structure |
publisher |
Cambridge University Press (CUP) |
publishDate |
2007 |
url |
http://dx.doi.org/10.1017/s0954102007000314 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102007000314 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctic Science |
genre_facet |
Antarc* Antarctic Antarctic Science |
op_source |
Antarctic Science volume 19, issue 2, page 239-244 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102007000314 |
container_title |
Antarctic Science |
container_volume |
19 |
container_issue |
2 |
container_start_page |
239 |
op_container_end_page |
244 |
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1792502853987729408 |