Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community
A field experiment investigating the effect of oil contamination on benthic microbial communities was conducted near Casey Station, East Antarctica. Defaunated sediment was treated with a mixture of Special Antarctic Blend diesel and lubricating oil and deployed in three different bays for eleven we...
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2005
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Online Access: | http://dx.doi.org/10.1017/s0954102005002786 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102005002786 |
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crcambridgeupr:10.1017/s0954102005002786 2024-03-03T08:37:41+00:00 Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community POWELL, SHANE M. RIDDLE, MARTIN J. SNAPE, IAN STARK, JONATHAN S. 2005 http://dx.doi.org/10.1017/s0954102005002786 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102005002786 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 17, issue 3, page 353-360 ISSN 0954-1020 1365-2079 Geology Ecology, Evolution, Behavior and Systematics Oceanography journal-article 2005 crcambridgeupr https://doi.org/10.1017/s0954102005002786 2024-02-08T08:34:50Z A field experiment investigating the effect of oil contamination on benthic microbial communities was conducted near Casey Station, East Antarctica. Defaunated sediment was treated with a mixture of Special Antarctic Blend diesel and lubricating oil and deployed in three different bays for eleven weeks. A molecular fingerprinting technique, denaturing gradient gel electrophoresis (DGGE), was used to investigate the microbial community structure. The variation between replicate samples within treatment groups indicates that the benthic microbial populations are very diverse and evenly distributed. Comparisons to determine the significance of both deployment location and hydrocarbon treatment showed that the greatest effect was from a combination of location and treatment. Detailed analysis suggests that subtle differences may be obscured by variability introduced by PCR and gel stages in DGGE, undermining this experimental approach. It is concluded that both location and hydrocarbon contamination influenced the development of the microbial communities but that the effect of hydrocarbon treatment varied with location. This has important implications for the design of future experiments on the effect of hydrocarbons on benthic communities, especially if it is intended to generalize the conclusions drawn from site specific studies. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Antarctica East Antarctica Cambridge University Press Antarctic East Antarctica Casey Station ENVELOPE(110.528,110.528,-66.282,-66.282) Antarctic Science 17 3 353 360 |
institution |
Open Polar |
collection |
Cambridge University Press |
op_collection_id |
crcambridgeupr |
language |
English |
topic |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
spellingShingle |
Geology Ecology, Evolution, Behavior and Systematics Oceanography POWELL, SHANE M. RIDDLE, MARTIN J. SNAPE, IAN STARK, JONATHAN S. Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community |
topic_facet |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
description |
A field experiment investigating the effect of oil contamination on benthic microbial communities was conducted near Casey Station, East Antarctica. Defaunated sediment was treated with a mixture of Special Antarctic Blend diesel and lubricating oil and deployed in three different bays for eleven weeks. A molecular fingerprinting technique, denaturing gradient gel electrophoresis (DGGE), was used to investigate the microbial community structure. The variation between replicate samples within treatment groups indicates that the benthic microbial populations are very diverse and evenly distributed. Comparisons to determine the significance of both deployment location and hydrocarbon treatment showed that the greatest effect was from a combination of location and treatment. Detailed analysis suggests that subtle differences may be obscured by variability introduced by PCR and gel stages in DGGE, undermining this experimental approach. It is concluded that both location and hydrocarbon contamination influenced the development of the microbial communities but that the effect of hydrocarbon treatment varied with location. This has important implications for the design of future experiments on the effect of hydrocarbons on benthic communities, especially if it is intended to generalize the conclusions drawn from site specific studies. |
format |
Article in Journal/Newspaper |
author |
POWELL, SHANE M. RIDDLE, MARTIN J. SNAPE, IAN STARK, JONATHAN S. |
author_facet |
POWELL, SHANE M. RIDDLE, MARTIN J. SNAPE, IAN STARK, JONATHAN S. |
author_sort |
POWELL, SHANE M. |
title |
Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community |
title_short |
Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community |
title_full |
Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community |
title_fullStr |
Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community |
title_full_unstemmed |
Location and DGGE methodology can influence interpretation of field experimental studies on the response to hydrocarbons by Antarctic benthic microbial community |
title_sort |
location and dgge methodology can influence interpretation of field experimental studies on the response to hydrocarbons by antarctic benthic microbial community |
publisher |
Cambridge University Press (CUP) |
publishDate |
2005 |
url |
http://dx.doi.org/10.1017/s0954102005002786 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102005002786 |
long_lat |
ENVELOPE(110.528,110.528,-66.282,-66.282) |
geographic |
Antarctic East Antarctica Casey Station |
geographic_facet |
Antarctic East Antarctica Casey Station |
genre |
Antarc* Antarctic Antarctic Science Antarctica East Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Science Antarctica East Antarctica |
op_source |
Antarctic Science volume 17, issue 3, page 353-360 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102005002786 |
container_title |
Antarctic Science |
container_volume |
17 |
container_issue |
3 |
container_start_page |
353 |
op_container_end_page |
360 |
_version_ |
1792500771709779968 |