The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate
In the Antarctic ciliate, Euplotes focardii , the heat-shock protein 70 (Hsp70) gene does not show any appreciable activation by a thermal stress. Yet, it is activated to appreciable transcriptional levels by oxidative and chemical stresses, thus implying that it evolved a mechanism of selective, st...
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Online Access: | http://dx.doi.org/10.1017/s0954102004001774 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102004001774 |
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crcambridgeupr:10.1017/s0954102004001774 2024-09-15T17:41:26+00:00 The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate LA TERZA, ANTONIETTA MICELI, CRISTINA LUPORINI, PIERANGELO 2004 http://dx.doi.org/10.1017/s0954102004001774 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102004001774 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 16, issue 1, page 23-28 ISSN 0954-1020 1365-2079 journal-article 2004 crcambridgeupr https://doi.org/10.1017/s0954102004001774 2024-07-03T04:01:16Z In the Antarctic ciliate, Euplotes focardii , the heat-shock protein 70 (Hsp70) gene does not show any appreciable activation by a thermal stress. Yet, it is activated to appreciable transcriptional levels by oxidative and chemical stresses, thus implying that it evolved a mechanism of selective, stress-specific response. A basic step in investigating this mechanism is the determination of the complete nucleotide sequence of the E. focardii Hsp70 gene. This gene contains a coding region specific for an Hsp70 protein that carries unique amino acid substitutions of potential significance for cold adaptation, and a 5' regulatory region that includes sequence motifs denoting two distinct types of stress-inducible promoters, known as “ H eat S hock E lements” (HSE) and “ St ress R esponse E lements” (StRE). From the study of the interactions of these regulatory elements with their specific transactivator factors we expect to shed light on the adaptive modifications that prevent the Hsp70 gene of E. focardii from responding to thermal stress while being responsive to other stresses. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Cambridge University Press Antarctic Science 16 1 23 28 |
institution |
Open Polar |
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Cambridge University Press |
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crcambridgeupr |
language |
English |
description |
In the Antarctic ciliate, Euplotes focardii , the heat-shock protein 70 (Hsp70) gene does not show any appreciable activation by a thermal stress. Yet, it is activated to appreciable transcriptional levels by oxidative and chemical stresses, thus implying that it evolved a mechanism of selective, stress-specific response. A basic step in investigating this mechanism is the determination of the complete nucleotide sequence of the E. focardii Hsp70 gene. This gene contains a coding region specific for an Hsp70 protein that carries unique amino acid substitutions of potential significance for cold adaptation, and a 5' regulatory region that includes sequence motifs denoting two distinct types of stress-inducible promoters, known as “ H eat S hock E lements” (HSE) and “ St ress R esponse E lements” (StRE). From the study of the interactions of these regulatory elements with their specific transactivator factors we expect to shed light on the adaptive modifications that prevent the Hsp70 gene of E. focardii from responding to thermal stress while being responsive to other stresses. |
format |
Article in Journal/Newspaper |
author |
LA TERZA, ANTONIETTA MICELI, CRISTINA LUPORINI, PIERANGELO |
spellingShingle |
LA TERZA, ANTONIETTA MICELI, CRISTINA LUPORINI, PIERANGELO The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate |
author_facet |
LA TERZA, ANTONIETTA MICELI, CRISTINA LUPORINI, PIERANGELO |
author_sort |
LA TERZA, ANTONIETTA |
title |
The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate |
title_short |
The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate |
title_full |
The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate |
title_fullStr |
The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate |
title_full_unstemmed |
The gene for the heat-shock protein 70 of Euplotes focardii, an Antarctic psychrophilic ciliate |
title_sort |
gene for the heat-shock protein 70 of euplotes focardii, an antarctic psychrophilic ciliate |
publisher |
Cambridge University Press (CUP) |
publishDate |
2004 |
url |
http://dx.doi.org/10.1017/s0954102004001774 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102004001774 |
genre |
Antarc* Antarctic Antarctic Science |
genre_facet |
Antarc* Antarctic Antarctic Science |
op_source |
Antarctic Science volume 16, issue 1, page 23-28 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102004001774 |
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Antarctic Science |
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16 |
container_issue |
1 |
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23 |
op_container_end_page |
28 |
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1810487606037708800 |