Accumulation of HSP70 in Deschampsia antarctica Desv. leaves under thermal stress
The aim of this study was to evaluate the hypothesis that Deschampsia antarctica Desv., the only grass of the Maritime Antarctic, responds to temperatures higher or lower than its optimum for photosynthesis (13°C), with the accumulation of heat shock proteins. The LT 50 was determined in plants accl...
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Cambridge University Press (CUP)
2003
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Online Access: | http://dx.doi.org/10.1017/s0954102003001366 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102003001366 |
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crcambridgeupr:10.1017/s0954102003001366 2024-03-03T08:38:48+00:00 Accumulation of HSP70 in Deschampsia antarctica Desv. leaves under thermal stress REYES, MARÍA ANTONIETA CORCUERA, LUIS J. CARDEMIL, LILIANA 2003 http://dx.doi.org/10.1017/s0954102003001366 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102003001366 en eng Cambridge University Press (CUP) https://www.cambridge.org/core/terms Antarctic Science volume 15, issue 3, page 345-352 ISSN 0954-1020 1365-2079 Geology Ecology, Evolution, Behavior and Systematics Oceanography journal-article 2003 crcambridgeupr https://doi.org/10.1017/s0954102003001366 2024-02-08T08:41:03Z The aim of this study was to evaluate the hypothesis that Deschampsia antarctica Desv., the only grass of the Maritime Antarctic, responds to temperatures higher or lower than its optimum for photosynthesis (13°C), with the accumulation of heat shock proteins. The LT 50 was determined in plants acclimated at 4°C (cold-acclimated plants) and in plants grown at 13°C (control plants) by membrane damage and by the activity of respiratory dehydrogenases. The LT 50 was 48.3°C in both groups of plants when determined by membrane damage. When the LT 50 was determined by the activity of respiratory dehydrogenases, this was, without significant difference, 47.8°C in cold-acclimated plants and 46.5°C in control plants. Western blot analyses were performed to investigate the accumulation of HSP70 at different temperatures. The optimal temperature for HSP70 accumulation was 35°C in cold-acclimated and control plants. Cold-acclimated plants subjected to a thermal stress of 35°C accumulated HSP70 protein more than control plants subjected to 35°C. After eight hours of continuous stress at 35°C, the maximum relative content of HSP70 in cold-acclimated plants was 1.9 fold higher than control plants. The threshold temperature for HSP70 accumulation was lower in cold-acclimated plants than in control plants. It is concluded that D. antarctica is able to tolerate heat stress with an LT 50 similar to those of heat tolerant plants and that the cold-acclimated plants accumulate higher levels of HSP70 than those grown at 13°C. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Antarctica Cambridge University Press Antarctic Antarctic Science 15 3 345 352 |
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Cambridge University Press |
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English |
topic |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
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Geology Ecology, Evolution, Behavior and Systematics Oceanography REYES, MARÍA ANTONIETA CORCUERA, LUIS J. CARDEMIL, LILIANA Accumulation of HSP70 in Deschampsia antarctica Desv. leaves under thermal stress |
topic_facet |
Geology Ecology, Evolution, Behavior and Systematics Oceanography |
description |
The aim of this study was to evaluate the hypothesis that Deschampsia antarctica Desv., the only grass of the Maritime Antarctic, responds to temperatures higher or lower than its optimum for photosynthesis (13°C), with the accumulation of heat shock proteins. The LT 50 was determined in plants acclimated at 4°C (cold-acclimated plants) and in plants grown at 13°C (control plants) by membrane damage and by the activity of respiratory dehydrogenases. The LT 50 was 48.3°C in both groups of plants when determined by membrane damage. When the LT 50 was determined by the activity of respiratory dehydrogenases, this was, without significant difference, 47.8°C in cold-acclimated plants and 46.5°C in control plants. Western blot analyses were performed to investigate the accumulation of HSP70 at different temperatures. The optimal temperature for HSP70 accumulation was 35°C in cold-acclimated and control plants. Cold-acclimated plants subjected to a thermal stress of 35°C accumulated HSP70 protein more than control plants subjected to 35°C. After eight hours of continuous stress at 35°C, the maximum relative content of HSP70 in cold-acclimated plants was 1.9 fold higher than control plants. The threshold temperature for HSP70 accumulation was lower in cold-acclimated plants than in control plants. It is concluded that D. antarctica is able to tolerate heat stress with an LT 50 similar to those of heat tolerant plants and that the cold-acclimated plants accumulate higher levels of HSP70 than those grown at 13°C. |
format |
Article in Journal/Newspaper |
author |
REYES, MARÍA ANTONIETA CORCUERA, LUIS J. CARDEMIL, LILIANA |
author_facet |
REYES, MARÍA ANTONIETA CORCUERA, LUIS J. CARDEMIL, LILIANA |
author_sort |
REYES, MARÍA ANTONIETA |
title |
Accumulation of HSP70 in Deschampsia antarctica Desv. leaves under thermal stress |
title_short |
Accumulation of HSP70 in Deschampsia antarctica Desv. leaves under thermal stress |
title_full |
Accumulation of HSP70 in Deschampsia antarctica Desv. leaves under thermal stress |
title_fullStr |
Accumulation of HSP70 in Deschampsia antarctica Desv. leaves under thermal stress |
title_full_unstemmed |
Accumulation of HSP70 in Deschampsia antarctica Desv. leaves under thermal stress |
title_sort |
accumulation of hsp70 in deschampsia antarctica desv. leaves under thermal stress |
publisher |
Cambridge University Press (CUP) |
publishDate |
2003 |
url |
http://dx.doi.org/10.1017/s0954102003001366 https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0954102003001366 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Science Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Science Antarctica |
op_source |
Antarctic Science volume 15, issue 3, page 345-352 ISSN 0954-1020 1365-2079 |
op_rights |
https://www.cambridge.org/core/terms |
op_doi |
https://doi.org/10.1017/s0954102003001366 |
container_title |
Antarctic Science |
container_volume |
15 |
container_issue |
3 |
container_start_page |
345 |
op_container_end_page |
352 |
_version_ |
1792507267131637760 |